TITLE

Intranasal Infection with Chlamydia abortus Induces Dose-Dependent Latency and Abortion in Sheep

AUTHOR(S)
Longbottom, David; Livingstone, Morag; Maley, Stephen; van der Zon, Arjan; Rocchi, Mara; Wilson, Kim; Wheelhouse, Nicholas; Dagleish, Mark; Aitchison, Kevin; Wattegedera, Sean; Nath, Mintu; Entrican, Gary; Buxton, David
PUB. DATE
February 2013
SOURCE
PLoS ONE;Feb2013, Vol. 8 Issue 2, p1
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: Latency is a key feature of the animal pathogen Chlamydia abortus, where infection remains inapparent in the non-pregnant animal and only becomes evident during a subsequent pregnancy. Often the first sign that an animal is infected is abortion occurring late in gestation. Despite this, little is understood of the underlying mechanisms that control latency or the recrudescence of infection that occurs during subsequent pregnancy. The aim of this study was to develop an experimental model of latency by mimicking the natural route of infection through the intranasal inoculation of non-pregnant sheep with C. abortus. Methodology/Principal Findings: Three groups of sheep (groups 1, 2 and 3) were experimentally infected with different doses of C. abortus (5×103, 5×105 and 5×107 inclusion forming units (IFU), respectively) prior to mating and monitored over 2 breeding cycles for clinical, microbiological, pathological, immunological and serological outcomes. Two further groups received either negative control inoculum (group 4a,b) or were inoculated subcutaneously on day 70 of gestation with 2×106 IFU C. abortus (group 5). Animals in groups 1, 2 and 5 experienced an abortion rate of 50–67%, while only one animal aborted in group 3 and none in group 4a,b. Pathological, microbiological, immunological and serological analyses support the view that the maternal protective immune response is influenced by initial exposure to the bacterium. Conclusions/Significance: The results show that intranasal administration of non-pregnant sheep with a low/medium dose of C. abortus results in a latent infection that leads in a subsequent pregnancy to infection of the placenta and abortion. In contrast a high dose stimulates protective immunity, resulting in a much lower abortion rate. This model will be useful in understanding the mechanisms of infection underlying latency and onset of disease, as well as in the development of novel therapeutics and vaccines for controlling infection.
ACCESSION #
87625716

 

Related Articles

  • The lipopolysaccharide core of Actinobacillus suis and its relationship to those of Actinobacillus pleuropneumoniae. Ganeshapillai, Jeyabarathy; Boncheff, Alexander G.; Slavic, Durda; MacInnes, Janet; Monteiro, Mario A. // Biochemistry & Cell Biology;Jun2011, Vol. 89 Issue 3, p351 

    The Gram-negative bacteria colonizes the upper respiratory and genital tracts of swine. Along with capsular polysaccharides, lipopolysaccharides (O-chain→core→lipid A~cell) are a main cell-surface component of A. suis. In this study, we determined that A. suis lipopolysaccharide...

  • Comparing the genomes of Helicobacter pylori clinical strain UM032 and Mice-adapted derivatives. Khosravi, Yalda; Rehvathy, Vellaya; Wei Yee Wee; Wang, Susana; Baybayan, Primo; Singh, Siddarth; Ashby, Meredith; Junxian Ong; Amoyo, Arlaine Anne; Shih Wee Seow; Siew Woh Choo; Perkins, Tim; Eng Guan Chua; Tay, Alfred; Marshall, Barry James; Mun Fai Loke; Khean Lee Goh; Pettersson, Sven; Vadivelu, Jamuna // Gut Pathogens; 

    Background: Helicobacter pylori is a Gram-negative bacterium that persistently infects the human stomach inducing chronic inflammation. The exact mechanisms of pathogenesis are still not completely understood. Although not a natural host for H. pylori, mouse infection models play an important...

  • Lipopolysaccharide-binding molecules: transporters, blockers and sensors. R. Chaby // Cellular & Molecular Life Sciences;Jul2004, Vol. 61 Issue 14, p1697 

    Lipopolysaccharide (LPS), a major component of the outer membrane of Gram-negative bacteria, can be beneficial to the host by activating the innate immune system, or harmful, by inducing inflammation, disseminated intravascular coagulation, multiple organ failure, shock and often death. On the...

  • Lipopolysaccharide-binding protein is required to combat a murine Gram-negative bacterial infection. Jack, Robert S.; Fan, Xiaolong // Nature;10/16/1997, Vol. 389 Issue 6652, p742 

    Presents research which investigated how lipopolysaccharide-binding protein (LBP) functions in vivo in LBP-deficient mice. Role of lipopolysaccharide (LPS) in immune system against Gram-negative bacteria; Behavior of LPS in vitro; LBP as not required in vitro for clearance of LPS from...

  • Structural modifications of bacterial lipopolysaccharide that facilitate Gram-negative bacteria evasion of host innate immunity. Matsuura, Motohiro // Frontiers in Immunology;May2013, Vol. 4, p1 

    Bacterial lipopolysaccharide (LPS), a cell wall component characteristic of Gram-negative bacteria, is a representative pathogen-associated molecular pattern that allows mammalian cells to recognize bacterial invasion and trigger innate immune responses. The polysaccharide moiety of LPS primary...

  • Downmodulation of Vaccine-Induced Immunity and Protection against the Intracellular Bacterium Francisella tularensis by the Inhibitory Receptor FcγRIIB. Franz, Brian J.; Li, Ying; Bitsaktsis, Constantine; Iglesias, Bibiana V.; Pham, Giang; Sunagar, Raju; Kumar, Sudeep; Gosselin, Edmund J. // Journal of Immunology Research;4/19/2015, Vol. 2015, p1 

    Fc gamma receptor IIB (FcγRIIB) is the only Fc gamma receptor (FcγR) which negatively regulates the immune response, when engaged by antigen- (Ag-) antibody (Ab) complexes. Thus, the generation of Ag-specific IgG in response to infection or immunization has the potential to downmodulate...

  • Altered T Lymphocyte Proliferation upon Lipopolysaccharide Challenge Ex Vivo. Poujol, Fanny; Monneret, Guillaume; Pachot, Alexandre; Textoris, Julien; Venet, Fabienne // PLoS ONE;12/7/2015, Vol. 10 Issue 12, p1 

    Context: Sepsis is characterized by the development of adaptive immune cell alterations, which intensity and duration are associated with increased risk of health-care associated infections and mortality. However, pathophysiological mechanisms leading to such lymphocyte dysfunctions are not...

  • Twardziel -- problem historyczny? Zatoński, Tomasz; Zalesska-Kręcicka, Maria; Kręcicki, Tomasz // Clinical Dermatology / Dermatologia Kliniczna;2007, Vol. 9 Issue 1, p49 

    We describe history, epidemiology, current geographic range, diagnostics, treatment and clinical pictures of rhinoscleroma. Respiratory scleroma is a chronic, progressive granulomatous disease of the respiratory tract. Disease develops slowly and suspiciously, sometimes for dozens of years. It...

  • Human Female Genital Tract Infection by the Obligate Intracellular Bacterium Chlamydia trachomatis Elicits Robust Type 2 Immunity. Vicetti Miguel, Rodolfo D.; Harvey, Stephen A. K.; LaFramboise, William A.; Reighard, Seth D.; Matthews, Dean B.; Cherpes, Thomas L. // PLoS ONE;Mar2013, Vol. 8 Issue 3, p1 

    While Chlamydia trachomatis infections are frequently asymptomatic, mechanisms that regulate host response to this intracellular Gram-negative bacterium remain undefined. This investigation thus used peripheral blood mononuclear cells and endometrial tissue from women with or without Chlamydia...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics