Human SCARB2 Transgenic Mice as an Infectious Animal Model for Enterovirus 71

Lin, Yi-Wen; Yu, Shu-Ling; Shao, Hsiao-Yun; Lin, Hsiang-Yin; Liu, Chia-Chyi; Hsiao, Kuang-Nan; Chitra, Ebenezer; Tsou, Yueh-Liang; Chang, Hsuen-Wen; Sia, Charles; Chong, Pele; Chow, Yen-Hung
February 2013
PLoS ONE;Feb2013, Vol. 8 Issue 2, p1
Academic Journal
Enterovirus 71 (EV71) and coxsackievirus (CVA) are the most common causative factors for hand, foot, and mouth disease (HFMD) and neurological disorders in children. Lack of a reliable animal model is an issue in investigating EV71-induced disease manifestation in humans, and the current clinical therapies are symptomatic. We generated a novel EV71-infectious model with hSCARB2-transgenic mice expressing the discovered receptor human SCARB2 (hSCARB2). The challenge of hSCARB2-transgenic mice with clinical isolates of EV71 and CVA16 resulted in HFMD-like and neurological syndromes caused by E59 (B4) and N2838 (B5) strains, and lethal paralysis caused by 5746 (C2), N3340 (C4), and CVA16. EV71 viral loads were evident in the tissues and CNS accompanied the upregulated pro-inflammatory mediators (CXCL10, CCL3, TNF-α, and IL-6), correlating to recruitment of the infiltrated T lymphocytes that result in severe diseases. Transgenic mice pre-immunized with live E59 or the FI-E59 vaccine was able to resist the subsequent lethal challenge with EV71. These results indicate that hSCARB2-transgenic mice are a useful model for assessing anti-EV71 medications and for studying the pathogenesis induced by EV71.


Related Articles

  • Coxsackievirus B3 Inhibits Antigen Presentation In Vivo, Exerting a Profound and Selective Effect on the MHC Class I Pathway. Kemball, Christopher C.; Harkins, Stephanie; Whitmire, Jason K.; Flynn, Claudia T.; Feuer, Ralph; Whitton, J. Lindsay // PLoS Pathogens;Oct2009, Vol. 5 Issue 10, p1 

    Many viruses encode proteins whose major function is to evade or disable the host T cell response. Nevertheless, most viruses are readily detected by host T cells, and induce relatively strong T cell responses. Herein, we employ transgenic CD4+ and CD8+ T cells as sensors to evaluate in vitro...

  • Circulating Coxsackievirus A16 Identified as Recombinant Type A Human Enterovirus, China. Zhao, Ke; Han, Xue; Wang, Guanjun; Hu, Wei; Zhang, Wenyan; Yu, Xiao-Fang // Emerging Infectious Diseases;Aug2011, Vol. 17 Issue 8, p1537 

    To determine the relationship of coxsackievirus A16 (CA16) to prototype CA16-G10, we conducted a phylogenetic analysis of circulating CA16 strains in China. Complex recombinant forms of CA16-related viruses involving multiple human enteroviruses, subgroup A (CA4, CA16, and enterovirus 71), are...

  • Animal models have little to teach us about Type 1 diabetes: 2. In opposition to this proposal. Leiter, E. H.; Von Herrath, M. // Diabetologia;Oct2004, Vol. 47 Issue 10, p1657 

    The article presents animal model in general, and rodent model of Type 1 diabetes in particular, that are imperfect reflections of human disease. The major rodent models of spontaneous Type I diabetes are the Nonohese Diabetic mouse and the BioBreeding rat. The latter includes both the...

  • Abnormalities in the functioning of adipocytes from R6/2 mice that are transgenic for the Huntington's disease mutation. Fain, John N. // Human Molecular Genetics;Jan2001, Vol. 10 Issue 2, p145 

    Examines abnormalities in the functioning of adipocytes from a transgenic mice model of Huntington's disease. Overall growth retardation; Enhancement of body fat accumulation; Ability of insulin to stimulate leptin release from isolated epididymal adipose tissues.

  • CD8 T Cells and Toxoplasma gondii : A New Paradigm. Gigley, Jason P.; Bhadra, Rajarshi; Khan, Imtiaz A. // Journal of Parasitology Research;2011, p1 

    CD8 T cells are essential for control of Toxoplasma gondii infection. Once activated they undergo differentiation into short-lived effector and memory precursor effector cells. As effector cells, CD8 T cells exert immune pressure on the parasite via production of inflammatory cytokines and...

  • PROGRESS IN UNDERSTANDING AUTOIMMUNE EXOCRINOPATHY USING THE NON-OBESE DIABETIC MOUSE: AN UPDATE. Cha, S.; Peck, A. B.; Humphreys-Beher, M. G. // Critical Reviews in Oral Biology & Medicine;Jan2002, Vol. 13 Issue 1, p5 

    Sjögren's Syndrome (SS) is a chronic autoimmune disease characterized by histological and functional alterations of salivary and lacrimal glands that result in a severe dryness of the mouth and the eyes. The etiology of SS has remained undefined despite investigators' significant efforts to...

  • Targeting Gene Expression to the Epidermis of Transgenic Mice: Potential Applications to Genetic Skin Disorders. Rothnagel, Joseph A.; Longley, Mary A.; Bundman, Donnie; Greenhalgh, David A.; Dominey, Andrea M.; Roop, Dennis R. // Journal of Investigative Dermatology;Nov90, Vol. 95 Issue 5, p59S 

    The ability to specifically target gene expression to the epidermis of transgenic mice offers the exciting possibility of creating animal models of certain skin disorders that are inherited in man. It may be possible to produce mouse models of dominantly inherited keratinization disorders by...

  • Spontaneous opticospinal encephalomyelitis in a double-transgenic mouse model of autoimmune T cell/B cell cooperation. Krishnamoorthy, Gurumoorthy; Lassmann, Hans; Wekerle, Hartmut; Holz, Andreas // Journal of Clinical Investigation;Sep2006, Vol. 116 Issue 9, p2385 

    We describe a double-transgenic mouse strain (opticospinal EAE [OSE] mouse) that spontaneously develops an EAE-like neurological syndrome closely resembling a human variant of multiple sclerosis, Devic disease (also called neuromyelitis optica). Like in Devic disease, the inflammatory,...

  • The terminator mouse: salvation for primary cell culture. Kabgani, Nazanin; Moeller, Marcus J // Kidney International;Nov2013, Vol. 84 Issue 5, p866 

    The Terminator had to come back from the future already several times in an effort to bring salvation to mankind. In the present issue of Kidney International, Guo et al. brought us a novel transgenic mouse model: the terminator mouse. This highly elegant mouse may facilitate significantly the...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics