TITLE

tmRNA Is Essential in Shigella flexneri

AUTHOR(S)
Ramadoss, Nitya S.; Zhou, Xin; Keiler, Kenneth C.
PUB. DATE
February 2013
SOURCE
PLoS ONE;Feb2013, Vol. 8 Issue 2, p1
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Nonstop mRNAs pose a challenge for bacteria, because translation cannot terminate efficiently without a stop codon. The trans-translation pathway resolves nonstop translation complexes by removing the nonstop mRNA, the incomplete protein, and the stalled ribosome. P1 co-transduction experiments demonstrated that tmRNA, a key component of the trans-translation pathway, is essential for viability in Shigella flexneri. tmRNA was previously shown to be dispensable in the closely related species Escherichia coli, because E. coli contains a backup system for trans-translation mediated by the alternative release factor ArfA. Genome sequence analysis showed that S. flexneri does not have a gene encoding ArfA. E. coli ArfA could suppress the requirement for tmRNA in S. flexneri, indicating that tmRNA is essential in S. flexneri because there is no functional backup system. These data suggest that resolution of nonstop translation complexes is required for most bacteria.
ACCESSION #
87625523

 

Related Articles

  • A-Site mRNA Cleavage Is Not Required for tmRNA-Mediated ssrA-Peptide Tagging. Janssen, Brian D.; Garza-Sánchez, Fernando; Hayes, Christopher S. // PLoS ONE;Nov2013, Vol. 8 Issue 11, p1 

    In Escherichia coli, prolonged translational arrest allows mRNA degradation into the A site of stalled ribosomes. The enzyme that cleaves the A-site codon is not known, but its activity requires RNase II to degrade mRNA downstream of the ribosome. This A-site mRNA cleavage process is thought to...

  • Multiple conversion between the genes encoding bacterial class-I release factors. Ishikawa, Sohta A.; Kamikawa, Ryoma; Inagaki, Yuji // Scientific Reports;8/14/2015, p12406 

    Bacteria require two class-I release factors, RF1 and RF2, that recognize stop codons and promote peptide release from the ribosome. RF1 and RF2 were most likely established through gene duplication followed by altering their stop codon specificities in the common ancestor of extant bacteria....

  • Gene expression: Controlled protein degradation in bacteria.  // Nature Methods;Jan2015, Vol. 12 Issue 1, p16 

    The article looks at the use of Mesoplasma florum transfer-messenger RNA (tmRNA) system for targeting protein-degradation in Escherichia coli and also mentions that the strategy is developed by D. E. Cameron and J. J. Collins.

  • Efficient generation of gene-modified pigs via injection of zygote with Cas9/sgRNA. Wang, Yong; Du, Yinan; Shen, Bin; Zhou, Xiaoyang; Li, Jian; Liu, Yu; Wang, Jianying; Zhou, Jiankui; Hu, Bian; Kang, Nannan; Gao, Jimin; Yu, Liqing; Huang, Xingxu; Wei, Hong // Scientific Reports;2/6/2015, p8256 

    Co-injection of zygotes with Cas9 mRNA and sgRNA has been proven to be an efficient gene-editing strategy for genome modification of different species. Genetic engineering in pigs holds a great promise in biomedical research. By co-injection of one-cell stage embryos with Cas9 mRNA and Npc1l1...

  • The complex of tmRNA-SmpB and EF-G on translocating ribosomes. Ramrath, David J. F.; Yamamoto, Hiroshi; Rother, Kristian; Wittek, Daniela; Pech, Markus; Mielke, Thorsten; Loerke, Justus; Scheerer, Patrick; Ivanov, Pavel; Teraoka, Yoshika; Shpanchenko, Olga; Nierhaus, Knud H.; Spahn, Christian M. T. // Nature;5/24/2012, Vol. 485 Issue 7399, p526 

    Bacterial ribosomes stalled at the 3? end of malfunctioning messenger RNAs can be rescued by transfer-messenger RNA (tmRNA)-mediated trans-translation. The SmpB protein forms a complex with the tmRNA, and the transfer-RNA-like domain (TLD) of the tmRNA then enters the A site of the ribosome....

  • Bacterial pathogenesis: Chlamydia gets a NUE look. Jermy, Andrew // Nature Reviews Microbiology;Sep2010, Vol. 8 Issue 9, p614 

    The article focuses on a study by M. E. Pennini and colleagues which observed a type III secretion system (T3SS) effectors produced by C. trachomatis. The study found that histone methyltransferase, which was targeted to the nucleus of the host cell, was one of the T3SS effectors produced by C....

  • Identification and Characterization of a Novel Shigella flexneri Serotype Yv in China. Sun, Qiangzheng; Lan, Ruiting; Wang, Jianping; Xia, Shengli; Wang, Yiting; Wang, Yan; Jin, Dong; Yu, Bo; Knirel, Yuriy A.; Xu, Jianguo // PLoS ONE;Jul2013, Vol. 8 Issue 7, p1 

    Shigella flexneri is the major cause of bacterial shigellosis in developing countries. S. flexneri is divided into at least 19 serotypes, the majority of which are modifications of the same basic O-antigen by glucosylation and/or O-acetylation of its sugar residues by phage encoded...

  • Freeing stalled ribosomes. Papatriantafyllou, Maria // Nature Reviews Molecular Cell Biology;May2012, Vol. 13 Issue 5, p280 

    The article discusses research being done on the release of stalled ribosomes by transfer-messenger ribonucleic acid (tmRNA) and references the article "Decoding in the absence of a codon by tmRNA and SmpB in the ribosome," by C. Neubauer and colleagues in the 2012 issue of "Science"

  • Using Chemical Approaches to Understand RNA Structure and Function in Biology. Stetsenko, Dmitry A.; van Aerschot, Arthur // Journal of Nucleic Acids;2012, p1 

    An introduction is presented in which the editor discusses various reports within the issue on topics including RNA structure-function relationships, RNA protein interaction and trans translation and the role of transfer-messenger RNA.

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics