Early and Efficient Detection of Mycobacterium tuberculosis in Sputum by Microscopic Observation of Broth Cultures

Kidenya, Benson R.; Kabangila, Rodrick; Peck, Robert N.; Mshana, Stephen E.; Webster, Lauren E.; Koenig, Serena P.; Johnson Jr., Warren D.; Fitzgerald, Daniel W.
February 2013
PLoS ONE;Feb2013, Vol. 8 Issue 2, p1
Academic Journal
Early, efficient and inexpensive methods for the detection of pulmonary tuberculosis are urgently needed for effective patient management as well as to interrupt transmission. These methods to detect M. tuberculosis in a timely and affordable way are not yet widely available in resource-limited settings. In a developing-country setting, we prospectively evaluated two methods for culturing and detecting M. tuberculosis in sputum. Sputum samples were cultured in liquid assay (micro broth culture) in microplate wells and growth was detected by microscopic observation, or in Löwenstein–Jensen (LJ) solid media where growth was detected by visual inspection for colonies. Sputum samples were collected from 321 tuberculosis (TB) suspects attending Bugando Medical Centre, in Mwanza, Tanzania, and were cultured in parallel. Pulmonary tuberculosis cases were diagnosed using the American Thoracic Society diagnostic standards. There were a total of 200 (62.3%) pulmonary tuberculosis cases. Liquid assay with microscopic detection detected a significantly higher proportion of cases than LJ solid culture: 89.0% (95% confidence interval [CI], 84.7% to 93.3%) versus 77.0% (95% CI, 71.2% to 82.8%) (p = 0.0007). The median turn around time to diagnose tuberculosis was significantly shorter for micro broth culture than for the LJ solid culture, 9 days (interquartile range [IQR] 7–13), versus 21 days (IQR 14–28) (p<0.0001). The cost for micro broth culture (labor inclusive) in our study was US $4.56 per sample, versus US $11.35 per sample for the LJ solid culture. The liquid assay (micro broth culture) is an early, feasible, and inexpensive method for detection of pulmonary tuberculosis in resource limited settings.


Related Articles

  • Whole Genome Sequencing of Mycobacterium tuberculosis Reveals Slow Growth and Low Mutation Rates during Latent Infections in Humans. Colangeli, Roberto; Arcus, Vic L.; Cursons, Ray T.; Ruthe, Ali; Karalus, Noel; Coley, Kathy; Manning, Shannon D.; Kim, Soyeon; Marchiano, Emily; Alland, David // PLoS ONE;Mar2014, Vol. 9 Issue 3, p1 

    Very little is known about the growth and mutation rates of Mycobacterium tuberculosis during latent infection in humans. However, studies in rhesus macaques have suggested that latent infections have mutation rates that are higher than that observed during active tuberculosis disease. Elevated...

  • Estratos de incidencia de tuberculosis en los municipios de Cuba: 1999--2002 y 2003--2006. Díaz, Alexander González; Soler, Kleich Pérez; Valdés, Lizet Sánchez; Matthys, Francine; Ochoa, Edilberto González; Van der Stuyft, Patrick // Revista Panamericana de Salud Publica;Oct2010, Vol. 28 Issue 4, p275 

    Objective. Identify Cuban municipalities with high, medium, and low incidence of tuberculosis (TB), compare incidence rates for the periods 1999.2002 and 2003.2006, and analyze distribution of the disease by population density and economic activity. Methods. TB incidence was calculated by...

  • A Field Evaluation of the Hardy TB MODS Kitâ„¢ for the Rapid Phenotypic Diagnosis of Tuberculosis and Multi-Drug Resistant Tuberculosis. Martin, Laura; Coronel, Jorge; Faulx, Dunia; Valdez, Melissa; Metzler, Mutsumi; Crudder, Chris; Castillo, Edith; Caviedes, Luz; Grandjean, Louis; Rodriguez, Mitzi; Friedland, Jon S.; Gilman, Robert H.; Moore, David A. J. // PLoS ONE;Sep2014, Vol. 9 Issue 9, p1 

    Background: Even though the WHO-endorsed, non-commercial MODS assay offers rapid, reliable TB liquid culture and phenotypic drug susceptibility testing (DST) at lower cost than any other diagnostic, uptake has been patchy. In part this reflects misperceptions about in-house assay quality...

  • Observational Studies on Evaluating the Safety and Adverse Effects of Traditional Chinese Medicine. Jung-Nein Lai; Jin-Ling Tang; Jung-Der Wang // Evidence-based Complementary & Alternative Medicine (eCAM);2013, Vol. 2013, p1 

    Background. This study aims to share our experiences when carrying out observational studies of traditional Chinese medicine (TCM). Methods. We have proactively monitored the safety profiles of Duhuo Jisheng Tang (DJT), Suan Zao Ren Tang (SZRT), and TMN-1. A list of adverse events (AEs),...

  • Clinical prediction model to aid emergency doctors managing febrile children at risk of serious bacterial infections: diagnostic study. Nijman, Ruud G.; Vergouwe, Yvonne; Thompson, Matthew; van Veen, Mirjam; van Meurs, Alfred H.J.; van der Lei, Johan; Steyerberg, Ewout W.; Moll, Henriette A.; Oostenbrink, Rianne // BMJ: British Medical Journal;4/27/2013, Vol. 346 Issue 7905, p15 

    The article discusses a study to investigate a clinical prediction model to help emergency doctors in managing febrile children at risk of serious bacterial infections. It informs that a validated prediction model including clinical signs and C reactive protein was used in estimating the...

  • Complexity and clarity in evidence use. Boaz, Annette; Gough, David // Evidence & Policy: A Journal of Research, Debate & Practice;Jan2012, Vol. 8 Issue 1, p3 

    An introduction to the journal is presented in which the editor discusses various reports within the issue on topics including the relevance of observational studies, examples of evidence use, and an analysis of the overlaps between public health intervention research (PHIR) and knowledge...

  • The Incidence Rate of Thyroid Cancer Among Women in Saudi Arabia: An Observational Descriptive Epidemiological Analysis of Data from Saudi Cancer Registry 2001-2008. Alghamdi, Ibrahim; Hussain, Issam; Alghamdi, Mohamed; Dohal, Ahlam; Almalki, Shaia; El-Sheemy, Mohammed // Journal of Immigrant & Minority Health;Jun2015, Vol. 17 Issue 3, p638 

    This study provides a descriptive epidemiological data of thyroid cancer cases diagnosed from 2001 to 2008 among Saudi women, including the frequency and percentage of cases, the crude incidence rate (CIR) and the age-standardised incidence rate (ASIR) stratified by the region and year of...

  • Paradoxical Reaction Syndrome Complicating Aural Infection Due to Mycohacterium tuberculosis during Therapy. Safdar, Amar; Brown, Arthur E.; Kraus, Dennis H.; Malkin, Mark // Clinical Infectious Diseases;3/1/2000, Vol. 30 Issue 3, p625 

    Discusses a paradoxical reaction syndrome complicating aural infection due to Mycobacterium tuberculosis during antituberculosis therapy. Case report; Treatment; Role of preemptive glucocorticosteroid therapy.

  • Complicated intra-abdominal infections in Europe: a comprehensive review of the CIAO study. Sartelli, Massimo; Catena, Fausto; Ansaloni, Luca; Leppaniemi, Ari; Taviloglu, Korhan; van Goor, Harry; Viale, Pierluigi; Vasco Lazzareschi, Daniel; Coccolini, Federico; Corbella, Davide; de Werra, Carlo; Marrelli, Daniele; Colizza, Sergio; Scibè, Rodolfo; Alis, Halil; Torer, Nurkan; Navarro, Salvador; Sakakushev, Boris; Massalou, Damien; Augustin, Goran // World Journal of Emergency Surgery;2012, Vol. 7 Issue 1, p36 

    The CIAO Study ("Complicated Intra-Abdominal infection Observational" Study) is a multicenter investigation performed in 68 medical institutions throughout Europe over the course of a 6-month observational period (January-June 2012). Patients with either community-acquired or...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics