TITLE

Detection of MicroRNAs in Archival Cytology Urine Smears

AUTHOR(S)
Simonato, Francesca; Ventura, Laura; Sartori, Nicola; Cappellesso, Rocco; Fassan, Matteo; Busund, Lill-Tove; Fassina, Ambrogio
PUB. DATE
February 2013
SOURCE
PLoS ONE;Feb2013, Vol. 8 Issue 2, p1
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
MicroRNAs’ dysregulation and profiling have been demonstrated to be clinically relevant in urothelial carcinoma (UC). Urine cytology is commonly used as the mainstay non-invasive test for secondary prevention and follow-up of UC patients. Ancillary tools are needed to support cytopathologists in the diagnosis of low-grade UC. The feasibility and reliability of microRNAs profiling by qRT-PCR analysis (miR-145 and miR-205) in archival routine urine cytology smears (affected by fixation/staining [Papanicolau] and room temperature storage) was tested in a series of 15 non-neoplastic and 10 UC urine specimens. Only samples with >5,000 urothelial cells and with <50% of inflammatory cells/red blood cells clusters were considered. Overall, a satisfactory amount of total RNA was obtained from all the considered samples (mean 1.27±1.43 µg, range 0.06–4.60 µg). Twenty nanograms of total RNA have been calculated to be the minimal total RNA concentration for reliable and reproducible miRNAs expression profiling analysis of archival cytological smears (slope = -3.4084; R-squared = 0.99; efficiency = 1.94). miR-145 and miR-205 were significantly downregulated in UC samples in comparison to non-tumor controls. These findings demonstrate that urine archival cytology smears are suitable for obtaining high-quality RNA to be used in microRNAs expression profiling. Further studies should investigate if miRNAs profiling can be successfully translated into clinical practice as diagnostic or prognostic markers.
ACCESSION #
87625494

 

Related Articles

  • Analysis of Tissue and Serum MicroRNA Expression in Patients with Upper Urinary Tract Urothelial Cancer. Kriebel, Stephanie; Schmidt, Doris; Holdenrieder, Stefan; Goltz, Diane; Kristiansen, Glen; Moritz, Rudolf; Fisang, Christian; Müller, Stefan C.; Ellinger, Jörg // PLoS ONE;Jan2015, Vol. 10 Issue 1, p1 

    Introduction: MicroRNAs play an important role in many human malignancies; so far, their expression remains to be studied in upper urinary tract urothelial cancer (UUTUC). Materials and Methods: The expression of eleven microRNAs (miR-10a, miR-21, miR-96, miR-135, miR-141, miR-182, miR-200b,...

  • MicroRNA and piRNA Profiles in Normal Human Testis Detected by Next Generation Sequencing. Yang, Qingling; Hua, Juan; Wang, Liu; Xu, Bo; Zhang, Huan; Ye, Nan; Zhang, Zhiqiang; Yu, Dexin; Cooke, Howard J.; Zhang, Yuanwei; Shi, Qinghua // PLoS ONE;Jun2013, Vol. 8 Issue 6, p1 

    Background: MicroRNAs (miRNAs) are the class of small endogenous RNAs that play an important regulatory role in cells by negatively affecting gene expression at transcriptional and post-transcriptional levels. There have been extensive studies aiming to discover miRNAs and to analyze their...

  • Positive urine cytology and carcinoma in situ prior to second transurethral resection of the bladder correlate with positive second resection histology and the need for subsequent cystectomy. Lodde, M.; Mayr, R.; Martini, T.; Comploj, E.; Palermo, S.; Trenti, E.; Hanspeter, E.; Fritsche, H.; Mian, C.; Pycha, A. // World Journal of Urology;Dec2012, Vol. 30 Issue 6, p841 

    Objective: A second transurethral resection of the bladder (TURB) is recommended for high-grade bladder cancer (BC) yet yields negative results in over half of the cases. Aim of this study was to identify prognostic indicators of a positive second TURB or the need for a subsequent cystectomy....

  • A non-invasive miRNA based assay to detect bladder cancer in cell-free urine. De Long, Jessica; Sullivan, Travis B.; Humphrey, John; Logvinenko, Tanya; Summerhayes, Kelly A.; Kozinn, Spencer; Harty, Niall; Summerhayes, Ian C.; Libertino, John A.; Holway, Antonia H.; Rieger-Christ, Kimberly M. // American Journal of Translational Research;2015, Vol. 7 Issue 11, p2500 

    RNA from cell-free urine was analyzed in an attempt to identify a microRNA (miRNA) profile that could be used as a non-invasive diagnostic assay to detect the presence of urothelial carcinoma of the bladder (UCB) and provide a discriminatory signature for different stages of progression. In...

  • microRNA response elements-regulated TRAIL expression shows specific survival-suppressing activity on bladder cancer. Youguang Zhao; Ying Li; Liang Wang; Hang Yang; Qingtang Wang; Haiyan Qi; Shadan Li; Peng Zhou; Ping Liang; Qiwu Wang; Xiaowei Li // Journal of Experimental & Clinical Cancer Research (17569966);2013, Vol. 32 Issue 1, p1 

    Background: Bladder transitional cell carcinoma greatly threatens human health all over the world. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) shows a strong apoptosis-inducing effect on a variety of cancer cells including bladder cancer. However, adenovirus-mediated TRAIL...

  • ROS generation via NOX4 and its utility in the cytological diagnosis of urothelial carcinoma of the urinary bladder.  // BMC Urology;2011, Vol. 11 Issue 1, p22 

    The article focuses on reactive oxygen species (ROS) generation through NADPH oxidase (NOX4) and its utility in the cytological diagnosis of urothelial carcinoma of the urinary bladder. It shows that ROS generation through NOX4 leads to urothelial carcinogenesis and cancer cell survival. It...

  • Bladder Cancer: Prognostic Genes and Signatures. Theodorescu, Dan // AUANews;2014, Vol. 19 Issue 4, p25 

    The article discusses the role of genetic expression and mutational signatures in bladder cancer prognosis. Findings from a research study which evaluated published gene signatures designed for diagnostic and prognostic purposes in urothelial cancer are discussed. An overview of two studies that...

  • Differential microRNA expression in aristolochic acid-induced upper urothelial tract cancers ex vivo. LE TAO; YIGANG ZENG; JUN WANG; ZHIHONG LIU; BING SHEN; JIFU GE; YONG LIU; YIFENG GUO; JIANXIN QIU // Molecular Medicine Reports;2015, Vol. 12 Issue 5, p6533 

    Aristolochic acid (AA) is a carcinogenic, mutagenic and nephrotoxic compound commonly isolated from members of the plant family of Aristolochiaceae (such as Aristolochia and Asarum) and used in Chinese herbal medicine. Use of AA and AA-containing plants causes chronic kidney disease (CKD) and...

  • MicroRNA Expression Signatures of Bladder Cancer Revealed by Deep Sequencing. Yonghua Han; Jiahao Chen; Xiaokun Zhao; Chaozhao Liang; Yong Wang; Liang Sun; Zhimao Jiang; Zhongfu Zhang; Ruilin Yang; Jing Chen; Zesong Li; Aifa Tang; Xianxin Li; Jiongxian Ye; Zhichen Guan; Yaoting Gui; Zhiming Cai // PLoS ONE;2011, Vol. 6 Issue 3, p1 

    Background: MicroRNAs (miRNAs) are a class of small noncoding RNAs that regulate gene expression. They are aberrantly expressed in many types of cancers. In this study, we determined the genome-wide miRNA profiles in bladder urothelial carcinoma by deep sequencing. Methodology/Principal...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics