Metagenomic Detection of Viral Pathogens in Spanish Honeybees: Co-Infection by Aphid Lethal Paralysis, Israel Acute Paralysis and Lake Sinai Viruses

Granberg, Fredrik; Vicente-Rubiano, Marina; Rubio-Guerri, Consuelo; Karlsson, Oskar E.; Kukielka, Deborah; Belák, Sándor; Sánchez-Vizcaíno, José Manuel
February 2013
PLoS ONE;Feb2013, Vol. 8 Issue 2, p1
Academic Journal
The situation in Europe concerning honeybees has in recent years become increasingly aggravated with steady decline in populations and/or catastrophic winter losses. This has largely been attributed to the occurrence of a variety of known and “unknown”, emerging novel diseases. Previous studies have demonstrated that colonies often can harbour more than one pathogen, making identification of etiological agents with classical methods difficult. By employing an unbiased metagenomic approach, which allows the detection of both unexpected and previously unknown infectious agents, the detection of three viruses, Aphid Lethal Paralysis Virus (ALPV), Israel Acute Paralysis Virus (IAPV), and Lake Sinai Virus (LSV), in honeybees from Spain is reported in this article. The existence of a subgroup of ALPV with the ability to infect bees was only recently reported and this is the first identification of such a strain in Europe. Similarly, LSV appear to be a still unclassified group of viruses with unclear impact on colony health and these viruses have not previously been identified outside of the United States. Furthermore, our study also reveals that these bees carried a plant virus, Turnip Ringspot Virus (TuRSV), potentially serving as important vector organisms. Taken together, these results demonstrate the new possibilities opened up by high-throughput sequencing and metagenomic analysis to study emerging new diseases in domestic and wild animal populations, including honeybees.


Related Articles

  • Genomics may help identify pathogens during outbreaks.  // Infectious Disease News;May2013, Vol. 26 Issue 5, p47 

    The article discusses research which revealed the possible use of metagenomics as a culture-independent approach in identifying pathogens during diarrheal disease outbreaks.

  • WATERBORNE PATHOGENS Pose Preventable Risks to Ventilated and Critically Ill Patients. Cervia, Joseph S.; Canonica, Francis P.; Ortolano, Girolamo A.; Maguire, James M. // RT: The Journal for Respiratory Care Practitioners;Oct2008, Vol. 21 Issue 10, p38 

    The article reports on the statistics from the United States Centers for Disease Control and Prevention (CDC) which showed that health care-associated infections (HAI) account for a estimated 1.7 million infections and 99,000 deaths yearly in American hospitals. It discusses that these...

  • Managed Pollinator CAP Coordinated Agricultural Project. Spivak, Marla // Bee Culture;Mar2010, Vol. 138 Issue 3, p41 

    The article presents a study that aims to distinguish the role of pathogens, pesticide and pests in causing the death of stationary honey bee colonies in the U.S. The study shows that honey bees at the stationary apiaries are being disclosed to different amounts of pesticides across and within...

  • Larvae must be under stress for this disease to become virulent. Collison, Clarence // Bee Culture;Jan2009, p29 

    The article discusses a study which determined whether the chalkboard, a lethal fungal disease caused by the pathogen Ascosphaera apis, develops in all brood stages of honey bees. It investigated the inoculation of honey bee eggs, larvae, prepupae, pupae with Ascosphaera apis spores. Also...

  • Comparison of Batrachochytrium dendrobatidis Testing Protocols for Helibenders (Cryptobranchus alleganiensis). Junge, Randall E.; Wanner, Mark; Ettling, Jeff; Briggler, Jeff // Journal of Herpetological Medicine & Surgery;2010, Vol. 20 Issue 4, p113 

    The hellbender (Cryptobranchus alleganiensis) has suffered a significant population decline and investigations into the cause are ongoing. One possible contributing factor is infection with the fungus Batrachochytrium dendrobatidis (Bd) as has been documented in many amphibian species. The goal...

  • Gut overgrowth harms the critically ill patient requiring treatment on the intensive care unit. VAN SAEN, HENDRIK; SILVESTRI, LUCIANO; TAYLOR, NIA; ZANDSTRA, DURK; DE LA CAL, MIGUEL; PETROS, ANDY // Signa Vitae;4/1/2012, Vol. 7 Issue 1, p7 

    Overgrowth is defined as ≥105 potential pathogens per ml of saliva and/or per g of faeces. There are six 'normal' potential pathogens carried by healthy individuals and nine 'abnormal' potential pathogens carried by individuals with underlying disease both chronic and acute. Surveillance...

  • Viral metagenomics demonstrates that domestic pigs are a potential reservoir for Ndumu virus. Masembe, Charles; Michuki, George; Onyango, Maria; Rumberia, Cecilia; Norling, Martin; Bishop, Richard P.; Djikeng, Appolinaire; Kemp, Stephen J.; Orth, Alan; Skilton, Robert A.; Ståhl, Karl; Fischer, Anne // Virology Journal;2012, Vol. 9 Issue 1, p218 

    Background: The rising demand for pork has resulted in a massive expansion of pig production in Uganda. This has resulted in increased contact between humans and pigs. Pigs can act as reservoirs for emerging infectious diseases. Therefore identification of potential zoonotic pathogens is...

  • Rapid metagenomic identification of viral pathogens in clinical samples by real-time nanopore sequencing analysis. Greninger, Alexander L.; Naccache, Samia N.; Federman, Scot; Guixia Yu; Mbala, Placide; Bres, Vanessa; Stryke, Doug; Bouquet, Jerome; Somasekar, Sneha; Linnen, Jeffrey M.; Dodd, Roger; Mulembakani, Prime; Schneider, Bradley S.; Muyembe-Tamfum, Jean-Jacques; Stramer, Susan L.; Chiu, Charles Y. // Genome Medicine;9/29/2015, Vol. 7 Issue 1, p1 

    We report unbiased metagenomic detection of chikungunya virus (CHIKV), Ebola virus (EBOV), and hepatitis C virus (HCV) from four human blood samples by MinION nanopore sequencing coupled to a newly developed, web-based pipeline for real-time bioinformatics analysis on a computational server or...

  • Population genomics reveals additive and replacing horizontal gene transfers in the emerging pathogen Dickeya solani. Khayi, Slimane; Blin, Pauline; Pédron, Jacques; Teik-Min Chong; Kok-Gan Chan; Moumni, Mohieddine; Hélias, Valérie; Van Gijsegem, Frédérique; Faure, Denis // BMC Genomics;10/14/2015, Vol. 16, p1 

    Background: Dickeya solani is an emerging pathogen that causes soft rot and blackleg diseases in several crops including Solanum tuberosum, but little is known about its genomic diversity and evolution. Results: We combined Illumina and PacBio technologies to complete the genome sequence of D....


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics