TITLE

Correlative In Vivo 2 Photon and Focused Ion Beam Scanning Electron Microscopy of Cortical Neurons

AUTHOR(S)
Maco, Bohumil; Holtmaat, Anthony; Cantoni, Marco; Kreshuk, Anna; Straehle, Christoph N.; Hamprecht, Fred A.; Knott, Graham W.
PUB. DATE
February 2013
SOURCE
PLoS ONE;Feb2013, Vol. 8 Issue 2, p1
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Correlating in vivo imaging of neurons and their synaptic connections with electron microscopy combines dynamic and ultrastructural information. Here we describe a semi-automated technique whereby volumes of brain tissue containing axons and dendrites, previously studied in vivo, are subsequently imaged in three dimensions with focused ion beam scanning electron microcopy. These neurites are then identified and reconstructed automatically from the image series using the latest segmentation algorithms. The fast and reliable imaging and reconstruction technique avoids any specific labeling to identify the features of interest in the electron microscope, and optimises their preservation and staining for 3D analysis.
ACCESSION #
87625431

 

Related Articles

  • How to Use FIB-SEM. Principe, Edward L. // R&D Magazine;Jul2006, Vol. 48 Issue 7, p33 

    The article offers a guide on the use of focused ion beam-scanning electron microscopy (FIB-SEM) data for 3-D reconstruction. The FIB-SEM tomographic method has demonstrated the ability to complete 3-D volumetric reconstruction at a resolution of 10 nanometer or better in all three dimensions....

  • Electrically driven optical antennas. Kern, Johannes; Kullock, René; Prangsma, Jord; Emmerling, Monika; Kamp, Martin; Hecht, Bert // Nature Photonics;Sep2015, Vol. 9 Issue 9, p582 

    Unlike radiowave antennas, so far optical nanoantennas cannot be fed by electrical generators. Instead, they are driven by light or indirectly via excited discrete states in active materials in their vicinity. Here we demonstrate the direct electrical driving of an in-plane optical antenna by...

  • High sensitivity resonance frequency measurements of individual microcantilevers using fiber optical interferometry. Duden, Thomas; Radmilovic, Velimir // Review of Scientific Instruments;Feb2009, Vol. 80 Issue 2, pN.PAG 

    We describe a setup for the resonance frequency measurement of individual microcantilevers. The setup displays both high spatial selectivity and sensitivity to specimen vibrations by utilizing a tapered fiber tip. The high sensitivity to specimen vibrations is achieved by the combination of...

  • MOST EXCITING TIME SINCE ABBE. DePalma, Angelo // Lab Manager Magazine;Mar2013, Vol. 8 Issue 2, p54 

    The article offers information on the Auriga FIB SEM platform, which combines focused ion beam (FIB) scanning electron microscopy (SEM), from Carl Zeiss AG.

  • Segmentation and Reconstruction of Cultured Neuron Skeleton. Donggang Yu; Pham, Tuan D.; Hong Yan; Wei Lai; Crane, Denis I. // AIP Conference Proceedings;11/2/2007, Vol. 952 Issue 1, p21 

    One approach to investigating neural death is through systematic studies of the changing morphology of cultured brain neurons in response to cellular challenges. Image segmentation and reconstruction methods developed to date to analyze such changes have been limited by the low contrast of...

  • An ultrastructure investigation of Argulus personatus Cunnington, 1913 (Crustacea: Branchiura) from Lake Tanganyika, northern Zambia. Quinton, Tam; Avenant-Oldewage, Annemarié; Williams, Ernest H. // African Zoology;Oct2005, Vol. 40 Issue 2, p301 

    Sixteen male and one female specimen of Argulus personatus Cunnington, 1913, were collected from Bathybates ferox Boulenger, 1898, from Lake Tanganyika in northern Zambia. Light and scanning electron microscopy (SEM) examinations documented a thickening of cuticle located on the dorsal surface...

  • Interface structure and strength of ultrasonically wedge bonded heavy aluminium wires in Si-based power modules. Pedersen, Kristian; Benning, David; Kristensen, Peter; Popok, Vladimir; Pedersen, Kjeld // Journal of Materials Science: Materials in Electronics;Jul2014, Vol. 25 Issue 7, p2863 

    In this paper the microscopical structure of wedge bonded interfaces is investigated, with a focus on what effect the power in the ultrasonic bonding and the initial microscopical structure of the $$Al$$ wire have on the quality of the bonding. The quality evaluation is based on mapping the...

  • Evolution of ion-induced nanoparticle arrays on GaAs surfaces. Kang, M.; Beskin, I.; Al-Heji, A. A.; Shende, O.; Huang, S.; Jeon, S.; Goldman, R. S. // Applied Physics Letters;5/5/2014, Vol. 104 Issue 18, p1 

    We have examined the evolution of irradiation-induced Ga nanoparticle (NP) arrays on GaAs surfaces. Focused-ion-beam irradiation of pre-patterned GaAs surfaces induces monotonic increases in the NP volume and aspect ratio up to a saturation ion dose, independent of NP location within the array....

  • POSSIBILITIES ON LATEST GENERATION SCANNING ELECTRON MICROSCOPES (SEM). PULFER, Kurt; DÜGGELIN, Marcel; MATHYS, Daniel; MORSON, Gianni; DÜRRENBERGER, Markus // Anadolu University Journal of Sciences & Technology;2009, Vol. 10 Issue 1, p57 

    During the last 10 years development of SEM with cold field emission guns working with low acceleration voltage lead to a substantial increase in resolving power. In addition the suppliers started to combine such SEM columns with focused ion beam columns (FIB). This dual column SEM / FIB allowed...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics