TITLE

Cellular Targets of Nitric Oxide in the Hippocampus

AUTHOR(S)
Bartus, Katalin; Pigott, Beatrice; Garthwaite, John
PUB. DATE
February 2013
SOURCE
PLoS ONE;Feb2013, Vol. 8 Issue 2, p1
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
In the hippocampus, as in many other CNS areas, nitric oxide (NO) participates in synaptic plasticity, manifested as changes in pre- and/or postsynaptic function. While it is known that these changes are brought about by cGMP following activation of guanylyl cyclase-coupled NO receptors attempts to locate cGMP by immunocytochemistry in hippocampal slices in response to NO have failed to detect the cGMP elevation where expected, i.e. in the pyramidal neurones. Instead, astrocytes, unidentified varicose fibres and GABA-ergic nerve terminals are reported to be the prominent NO targets, raising the possibility that NO acts indirectly via other cells. We have re-investigated the distribution of cGMP generated in response to endogenous and exogenous NO in hippocampal slices using immunohistochemistry and new conditions designed to optimise cGMP accumulation and, hence, its detectability. The conditions included use of tissue from the developing rat hippocampus, a potent inhibitor of phosphodiesterase-2, and an allosteric enhancer of the NO-receptive guanylyl cyclase. Under these conditions, cGMP was formed in response to endogenous NO and was found in a population of pyramidal cell somata in area CA3 and subiculum as well as in structures described previously. The additional presence of exogenous NO resulted in hippocampal cGMP reaching the highest level recorded for brain tissue (1700 pmol/mg protein) and in cGMP immunolabelling throughout the pyramidal cell layer. Populations of axons and interneurones were also stained. According with these results, immunohistochemistry for the common NO receptor β1-subunit indicated widespread expression. A similar staining pattern for the α1-subunit with an antibody used previously in the hippocampus and elsewhere, however, proved to be artefactual. The results indicate that the targets of NO in the hippocampus are more varied and extensive than previous evidence had suggested and, in particular, that the pyramidal neurones participating in NO-dependent synaptic plasticity are direct NO targets.
ACCESSION #
87625354

 

Related Articles

  • Involvement of nitric oxide-soluble guanylyl cyclase pathway in the control of maximal dentate gyrus activation in the rat. Sardo, P.; Carletti, F.; D’Agostino, S.; Rizzo, V.; Ferraro, G. // Journal of Neural Transmission;Dec2006, Vol. 113 Issue 12, p1855 

    Nitric oxide/soluble Guanylyl cyclase (NO/sGC) pathway on the maximal dentate gyrus activation (MDA) was studied in rats. The cerebral NO levels were modified by administrating 7-Nitroindazole (7-NI), a selective inhibitor of neuronal NOS, and L-arginine, a precursor of the synthesis of NO....

  • Strategies to increase nitric oxide signalling in cardiovascular disease. Lundberg, Jon O.; Gladwin, Mark T.; Weitzberg, Eddie // Nature Reviews Drug Discovery;Sep2015, Vol. 14 Issue 9, p623 

    Nitric oxide (NO) is a key signalling molecule in the cardiovascular, immune and central nervous systems, and crucial steps in the regulation of NO bioavailability in health and disease are well characterized. Although early approaches to therapeutically modulate NO bioavailability failed in...

  • Organization of cell assemblies in the hippocampus. Harris, Kenneth D.; Csicsvari, Jozsef; Hirase, Hajime; Dragoi, George; Buzsáki, György // Nature;7/31/2003, Vol. 424 Issue 6948, p552 

    Neurons can produce action potentials with high temporal precision[SUP1]. A fundamental issue is whether, and how, this capability is used in information processing. According to the 'cell assembly' hypothesis, transient synchrony of anatomically distributed groups of neurons underlies...

  • Regulation of survival in adult hippocampal and glioblastoma stem cell lineages by the homeodomain-only protein HOP. De Toni, Arianna; Zbinden, Marie; Epstein, Jonathan A.; Altaba, Ariel Ruiz; Prochiantz, Alain; Caillé, Isabelle // Neural Development;2008, Vol. 3, Special section p1 

    Background: Homeodomain proteins play critical roles in shaping the development of the embryonic central nervous system in mammals. After birth, neurogenic activities are relegated to stem cell niches, which include the subgranular layer of the dentate gyrus of the hippocampus. Here, we have...

  • Drugs that Activate Specific Nitric Oxide Sensitive Guanylyl Cyclase Isoforms Independent of Nitric Oxide Release. Behrends, Sonke // Current Medicinal Chemistry;Feb2003, Vol. 10 Issue 4, p291 

    Nitric oxide (NO) releasing drugs have helped patients suffering from angina pectoris for more than a century. In the 1970s NO-sensitive guanylyl cyclase was identified as the target of NO. Since then, three different isoforms of the enzyme have been identified. All NO-releasing drugs act by...

  • Nitric oxide-induced calcium release: activation of type 1 ryanodine receptor, a calcium release channel, through non-enzymatic post-translational modification by nitric oxide. Kakizawa, Sho // Frontiers in Endocrinology;Oct2013, Vol. 4, p1 

    Nitric oxide (NO) is a typical gaseous messenger involved in a wide range of biological processes. In our classical knowledge, effects of NO are largely achieved by activation of soluble guanylyl cyclase to form cyclic guanosine-3′, 5′-monophosphate. However, emerging evidences have...

  • Is Encephalopathy a Mechanism to Renew Sulfate in Autism? Seneff, Stephanie; Lauritzen, Ann; Davidson, Robert M.; Lentz-Marino, Laurie // Entropy;Jan2013, Vol. 15 Issue 1, p372 

    This paper makes two claims: (1) autism can be characterized as a chronic lowgrade encephalopathy, associated with excess exposure to nitric oxide, ammonia and glutamate in the central nervous system, which leads to hippocampal pathologies and resulting cognitive impairment, and (2),...

  • Nitric oxide-cyclic GMP pathway with some emphasis on cavernosal contractility. Ghalayini, IF // International Journal of Impotence Research;Dec2004, Vol. 16 Issue 6, p459 

    Nitric oxide (NO) is formed from the conversion of L-arginine by nitric oxide synthase (NOS), which exists in three isoforms: neuronal (nNOS), endothelial (eNOS), and inducible (iNOS). nNOS is expressed in penile neurons innervating the corpus cavernosum, and eNOS protein expression has been...

  • Expression of NOS1 and soluble guanylyl cyclase by human kidney epithelial cells: Morphological evidence for an autocrine/paracrine action of nitric oxide. Jarry, Anne; Renaudin, Karine; Denis, Marc G.; Robard, Myriam; Buffin-Meyer, Bénédicte; Karam, Georges; Buzelin, Françoise; Paris, Hervé; Laboisse, Christian L.; Vallette, Geneviève // Kidney International;Jul2003, Vol. 64 Issue 1, p170 

    Expression of NOS1 and soluble guanylyl cyclase by human kidney epithelial cells: Morphological evidence for an autocrine/paracrine action of nitric oxide. Background. Nitric oxide plays an important role in the kidney through effects on both renal hemodynamics and tubular functions. Tubular...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics