Neurogenesis of Retinal Ganglion Cells Is Not Essential to Visual Functional Recovery after Optic Nerve Injury in Adult Zebrafish

Zou, Suqi; Tian, Chen; Ge, Shuchao; Hu, Bing
February 2013
PLoS ONE;Feb2013, Vol. 8 Issue 2, p1
Academic Journal
Zebrafish central nervous system (CNS) possesses a strong neural regeneration ability to restore visual function completely after optic nerve injury (ONI). However, whether neurogenesis of retinal ganglion cell (RGC) contributes to functional recovery remains controversial. Our quantitative analysis of RGCs in different ONI models showed that almost all RGCs survived in optic nerve crush (ONC) model; while over 90% of RGCs survived in the first 2 weeks with 75% remaining after 7 weeks in optic nerve transection (ONT) model. Retrograde labeling from tectum revealed a surprising regeneration rate, with over 90% and over 50% of RGCs regrowing axons to tectum at the first week in ONC and ONT model respectively. In the latter one, the number of regenerative RGCs after 4 weeks had no significant difference from the control group. As for neurogenesis, newborn RGCs were rarely detected either by double retrograde labeling or BrdU marker. Since few RGCs died, microglia number showed a temporary increase at 3 days post injury (dpi) and a decrease at 14 dpi. Finally, myelin structure within retina kept integrity and optomotor response (OMR) test demonstrated visual functional restoration at 5 weeks post injury (wpi). In conclusion, our results have directly shown that RGC survival and axon regrowth are responsible for functional recovery after ONI in adult zebrafish.


Related Articles

  • Expression of PirB Protein in Intact and Injured Optic Nerve and Retina of Mice. Cai, Xiaofeng; Yuan, Rongdi; Hu, Zheng; Chen, Chunlin; Yu, Jun; Zheng, Zheng; Ye, Jian // Neurochemical Research;Mar2012, Vol. 37 Issue 3, p647 

    The aim of this study was to investigate the expression of PirB protein in intact mice ON (optic nerve) and retina, and to evaluate its change after ON injury. The mouse ON crush model was established. The immunohistochemistry and western blot were used to detect PirB expression. We discovered...

  • Rho-ROCK Inhibitors as Emerging Strategies to Promote Nerve Regeneration. Kubo, Takekazu; Hata, Katsuhiko; Yamaguchi, Atsushi; Yamashita, Toshihide // Current Pharmaceutical Design;Aug2007, Vol. 13 Issue 24, p2493 

    Several myelin-associated proteins in the central nervous system (CNS) have been identified as inhibitors of axonal regeneration following the injury of the adult vertebrate CNS. Among these inhibitors, myelin-associated glycoprotein (MAG), Nogo, and oligodendrocyte- myelin glycoprotein (OMgp)...

  • Ncam1a and Ncam1b: Two carriers of polysialic acid with different functions in the developing zebrafish nervous system. Langhauser, Melanie; Ustinova, Jana; Rivera-Milla, Eric; Ivannikov, Darja; Seidl, Carmen; Slomka, Christin; Finne, Jukka; Yoshihara, Yoshihiro; Bastmeyer, Martin; Bentrop, Joachim // Glycobiology;Feb2012, Vol. 22 Issue 2, p196 

    Polysialic acid (polySia) is mainly described as a glycan modification of the neural cell adhesion molecule NCAM1. PolySia-NCAM1 has multiple functions during the development of vertebrate nervous systems including axon extension and fasciculation. Phylogenetic analyses reveal the presence of...

  • Effects of Nitric Oxide on Neuromuscular Properties of Developing Zebrafish Embryos. Jay, Michael; Bradley, Sophie; McDearmid, Jonathan Robert // PLoS ONE;Jan2014, Vol. 9 Issue 1, p1 

    Nitric oxide is a bioactive signalling molecule that is known to affect a wide range of neurodevelopmental processes. However, its functional relevance to neuromuscular development is not fully understood. Here we have examined developmental roles of nitric oxide during formation and maturation...

  • Identification of the Mind Bomb1 Interaction Domain in Zebrafish DeltaD. Palardy, Gregory; Chitnis, Ajay B. // PLoS ONE;May2015, Vol. 10 Issue 5, p1 

    Ubiquitylation promotes endocytosis of the Notch ligands like Delta and Serrate and is essential for them to effectively activate Notch in a neighboring cell. The RING E3 ligase Mind bomb1 (Mib1) ubiquitylates DeltaD to facilitate Notch signaling in zebrafish. We have identified a domain in the...

  • Characterization of Proliferating Neural Progenitors after Spinal Cord Injury in Adult Zebrafish. Hui, Subhra Prakash; Nag, Tapas Chandra; Ghosh, Sukla // PLoS ONE;12/2/2015, Vol. 10 Issue 12, p1 

    Zebrafish can repair their injured brain and spinal cord after injury unlike adult mammalian central nervous system. Any injury to zebrafish spinal cord would lead to increased proliferation and neurogenesis. There are presences of proliferating progenitors from which both neuronal and glial...

  • The Role of inab in Axon Morphology of an Identified Zebrafish Motoneuron. Van Ryswyk, Liesl; Simonson, Levi; Eisen, Judith S. // PLoS ONE;Feb2014, Vol. 9 Issue 2, p1 

    The ability of an animal to move and to interact with its environment requires that motoneurons correctly innervate specific muscles. Although many genes that regulate motoneuron development have been identified, our understanding of motor axon branching remains incomplete. We used...

  • TISSUE REGENERATION in the Classroom! Hassoun, Lama; Hable, Whitney; Payne-Ferreira, Tracie // American Biology Teacher (National Association of Biology Teache;Nov/Dec2008, Vol. 70 Issue 9, p546 

    This article describes a science experiment that illustrates the process of cellular regeneration in the Danio rerio or zebrafish. The cellular process behind the regeneration of cells in a zebrafish is assessed. An expanded version of the experiment is presented that allows students to observe...

  • Mutations in gfpt1 and skiv2l2 Cause Distinct Stage-Specific Defects in Larval Melanocyte Regeneration in Zebrafish. Chao-Tsung Yang; Hindes, Anna E.; Hultman, Keith A.; Johnson, Stephen L. // PLoS Genetics;Jun2007, Vol. 3 Issue 6, pe88 

    The establishment of a single cell type regeneration paradigm in the zebrafish provides an opportunity to investigate the genetic mechanisms specific to regeneration processes. We previously demonstrated that regeneration melanocytes arise from cell division of the otherwise quiescent melanocyte...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics