Differential Activity of Drosophila Hox Genes Induces Myosin Expression and Can Maintain Compartment Boundaries

Curt, Jesús R.; de Navas, Luis F.; Sánchez-Herrero, Ernesto
February 2013
PLoS ONE;Feb2013, Vol. 8 Issue 2, p1
Academic Journal
Compartments are units of cell lineage that subdivide territories with different developmental potential. In Drosophila, the wing and haltere discs are subdivided into anterior and posterior (A/P) compartments, which require the activity of Hedgehog, and into dorsal and ventral (D/V) compartments, needing Notch signaling. There is enrichment in actomyosin proteins at the compartment boundaries, suggesting a role for these proteins in their maintenance. Compartments also develop in the mouse hindbrain rhombomeres, which are characterized by the expression of different Hox genes, a group of genes specifying different structures along their main axis of bilaterians. We show here that the Drosophila Hox gene Ultrabithorax can maintain the A/P and D/V compartment boundaries when Hedgehog or Notch signaling is compromised, and that the interaction of cells with and without Ultrabithorax expression induces high levels of non-muscle myosin II. In the absence of Ultrabithorax there is occasional mixing of cells from different segments. We also show a similar role in cell segregation for the Abdominal-B Hox gene. Our results suggest that the juxtaposition of cells with different Hox gene expression leads to their sorting out, probably through the accumulation of non-muscle myosin II at the boundary of the different cell territories. The increase in myosin expression seems to be a general mechanism used by Hox genes or signaling pathways to maintain the segregation of different groups of cells.


Related Articles

  • SIGNAL TRANSDUCTION: Top-Notch result. Wood, Heather // Nature Reviews Neuroscience;Jan2002, Vol. 3 Issue 1, p8 

    Discusses notch signal transduction. Factor which activate notch signaling in Drosophila neuroectoderm; Functionality of Notch genes; Potential reason for the late differentiation of neuroectodermal cells.

  • arrow encodes an LDL-receptor-related protein essential for Wingless signalling. Wehrli, Marcel; Dougan, Scott T.; Caldwell, Kim; O'Keefe, Louise; Schwartz, Stephanie; Vaizel-Ohayon, Dalit; Schejter, Eyal; Tomlinson, Andrew; DiNardo, Stephen // Nature;9/28/2000, Vol. 407 Issue 6803, p527 

    Describes arrow, a low-density lipoprotein (LDL)-receptor-related protein (LRP). This gene, which is necessary for all Wingless signalling events in Drosophila; Description of the arrow gene's function; Similarity to murine and human LRP5 and LRP6; Suggestion of a conserved function for this...

  • From Dynamic Expression Patterns to Boundary Formation in the Presomitic Mesoderm. Tiedemann, Hendrik B.; Schneltzer, Elida; Zeiser, Stefan; Hoesel, Bastian; Beckers, Johannes; Przemeck, Gerhard K. H.; De Angelis, Martin Hrabě // PLoS Computational Biology;Jun2012, Vol. 8 Issue 6, p1 

    The segmentation of the vertebrate body is laid down during early embryogenesis. The formation of signaling gradients, the periodic expression of genes of the Notch-, Fgf- and Wnt-pathways and their interplay in the unsegmented presomitic mesoderm (PSM) precedes the rhythmic budding of nascent...

  • Small molecules intercept Notch signaling and the early secretory pathway. Krämer, Andreas; Mentrup, Torben; Kleizen, Bertrand; Rivera-Milla, Eric; Reichenbach, Daniela; Enzensperger, Christoph; Nohl, Richard; Täuscher, Eric; Görls, Helmar; Ploubidou, Aspasia; Englert, Christoph; Werz, Oliver; Arndt, Hans-Dieter; Kaether, Christoph // Nature Chemical Biology;Nov2013, Vol. 9 Issue 11, p731 

    Notch signaling has a pivotal role in numerous cell-fate decisions, and its aberrant activity leads to developmental disorders and cancer. To identify molecules that influence Notch signaling, we screened nearly 17,000 compounds using automated microscopy to monitor the trafficking and...

  • The EGF Repeat-Specific O-GlcNAc-Transferase Eogt Interacts with Notch Signaling and Pyrimidine Metabolism Pathways in Drosophila Müller, Reto; Jenny, Andreas; Stanley, Pamela // PLoS ONE;May2013, Vol. 8 Issue 5, p1 

    The O-GlcNAc transferase Eogt modifies EGF repeats in proteins that transit the secretory pathway, including Dumpy and Notch. In this paper, we show that the Notch ligands Delta and Serrate are also substrates of Eogt, that mutation of a putative UDP-GlcNAc binding DXD motif greatly reduces...

  • Sharp boundaries of Dpp signalling trigger local cell death required for Drosophila leg morphogenesis. Manjón, Cristina; Sánchez-Herrero, Ernesto; Suzanne, Magali // Nature Cell Biology;Jan2007, Vol. 9 Issue 1, p57 

    Morphogens are secreted signalling molecules that govern many developmental processes. In the Drosophila wing disc, the transforming growth factor β (TGFβ) homologue Decapentaplegic (Dpp) forms a smooth gradient and specifies cell fate by conferring a defined value of morphogen activity....

  • Model-Based Analysis for Qualitative Data: An Application in Drosophila Germline Stem Cell Regulation. Pargett, Michael; Rundell, Ann E.; Buzzard, Gregery T.; Umulis, David M. // PLoS Computational Biology;Mar2014, Vol. 10 Issue 3, p1 

    Discovery in developmental biology is often driven by intuition that relies on the integration of multiple types of data such as fluorescent images, phenotypes, and the outcomes of biochemical assays. Mathematical modeling helps elucidate the biological mechanisms at play as the networks become...

  • The influence of morphogene Wg on the formation of an ectopic eye in Drosophila melanogaster. Kopyl, S.; Dubatolova, T.; Volkova, E.; Marilovtseva, E.; Omel'yanchuk, L. // Russian Journal of Genetics;Aug2011, Vol. 47 Issue 8, p907 

    Due to the ectopic expression of the ey gene in the wing imaginal disc under the action of the 1096-Gal4 driver, a part of the wing disc cells change their fate and become eye cells. Ectopic eyes are induced in definite regions of the wing disc and form a stable pattern on the wing of an adult...

  • Toll Mediated Infection Response Is Altered by Gravity and Spaceflight in Drosophila. Taylor, Katherine; Kleinhesselink, Kurt; George, Michael D.; Morgan, Rachel; Smallwood, Tangi; Hammonds, Ann S.; Fuller, Patrick M.; Saelao, Perot; Alley, Jeff; Gibbs, Allen G.; Hoshizaki, Deborah K.; von Kalm, Laurence; Fuller, Charles A.; Beckingham, Kathleen M.; Kimbrell, Deborah A. // PLoS ONE;Jan2014, Vol. 9 Issue 1, p1 

    Space travel presents unlimited opportunities for exploration and discovery, but requires better understanding of the biological consequences of long-term exposure to spaceflight. Immune function in particular is relevant for space travel. Human immune responses are weakened in space, with...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics