TITLE

Early Activation of Rat Skeletal Muscle IL-6/STAT1/STAT3 Dependent Gene Expression in Resistance Exercise Linked to Hypertrophy

AUTHOR(S)
Begue, Gwénaëlle; Douillard, Aymeric; Galbes, Olivier; Rossano, Bernadette; Vernus, Barbara; Candau, Robin; Py, Guillaume
PUB. DATE
February 2013
SOURCE
PLoS ONE;Feb2013, Vol. 8 Issue 2, p1
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Cytokine interleukin-6 (IL-6) is an essential regulator of satellite cell-mediated hypertrophic muscle growth through the transcription factor signal transducer and activator of transcription 3 (STAT3). The importance of this pathway linked to the modulation of myogenic regulatory factors expression in rat skeletal muscle undergoing hypertrophy following resistance exercise, has not been investigated. In this study, the phosphorylation and nuclear localization of STAT3, together with IL-6/STAT3-responsive gene expression, were measured after both a single bout of resistance exercise and 10 weeks of training. Flexor Digitorum Profundus muscle samples from Wistar rats were obtained 2 and 6 hours after a single bout of resistance exercise and 72 h after the last bout of either 2, 4, or 10 weeks of resistance training. We observed an increase in IL-6 and SOCS3 mRNAs concomitant with phosphorylation of STAT1 and STAT3 after 2 and 6 hours of a single bout of exercise (p<0.05). STAT3-dependent early responsive genes such as CyclinD1 and cMyc were also upregulated whereas MyoD and Myf5 mRNAs were downregulated (p<0.05). BrdU-positive satellite cells increased at 2 and 6 hours after exercise (p<0.05). Muscle fiber hypertrophy reached up to 100% after 10 weeks of training and the mRNA expression of Myf5, c-Myc and Cyclin-D1 decreased, whereas IL-6 mRNA remained upregulated. We conclude that the IL-6/STAT1/STAT3 signaling pathway and its responsive genes after a single bout of resistance exercise are an important event regulating the SC pool and behavior involved in muscle hypertrophy after ten weeks of training in rat skeletal muscle.
ACCESSION #
87625235

 

Related Articles

  • Dynamics of the STAT3 Transcription Factor: Nuclear Import Dependent on Ran and Importin-β1. Cimica, Velasco; Hui-Chen Chen; Iyer, Janaki K.; Reich, Nancy C. // PLoS ONE;2011, Vol. 6 Issue 5, p1 

    The signal transducer and activator of transcription-3 (STAT3) induces transcription of genes that control differentiation, inflammation, proliferation, and tumor cell invasion. Cytokines such as interleukin-6 and interferon stimulate the specific tyrosine phosphorylation of STAT3, which confers...

  • High mobility group box 1 induces synoviocyte proliferation in rheumatoid arthritis by activating the signal transducer and activator transcription signal pathway. Guo, Hui-Fang; Liu, Shu-Xia; Zhang, Yu-Jun; Liu, Qing-Juan; Hao, Jun; Gao, Li-Xia // Clinical & Experimental Medicine;Jun2011, Vol. 11 Issue 2, p65 

    Recently, it was discovered that high mobility group box chromosomal protein 1 (HMGB1) acts as a potent pro-inflammatory cytokine that is released into the extracellular milieu. Signal transducer and activator of transcription (STAT) proteins play an important role in cytokine signaling. Some...

  • IL-1β and TNFα-initiated IL-6—STAT3 pathway is critical in mediating inflammatory cytokines and RANKL expression in inflammatory arthritis. Mori, Tomoaki; Miyamoto, Takeshi; Yoshida, Hideyuki; Asakawa, Mayoko; Kawasumi, Miyuri; Kobayashi, Takashi; Morioka, Hideo; Chiba, Kazuhiro; Toyama, Yoshiaki; Yoshimura, Akihiko // International Immunology;Nov2011, Vol. 23 Issue 11, p701 

    Rheumatoid arthritis (RA) is a chronic inflammatory disease that causes irreversible joint damage and significant disability. However, the fundamental mechanisms underlying how inflammation and joint destruction in RA develop and are sustained chronically remain largely unknown. Here, we show...

  • High force development augments skeletal muscle signalling in resistance exercise modes equalized for time under tension. Gehlert, Sebastian; Suhr, Frank; Gutsche, Katrin; Willkomm, Lena; Kern, Julia; Jacko, Daniel; Knicker, Axel; Schiffer, Thorsten; Wackerhage, Henning; Bloch, Wilhelm // Pflugers Archiv European Journal of Physiology;Jun2015, Vol. 467 Issue 6, p1343 

    How force development and time under tension (TUT) during resistance exercise (RE) influence anabolic signalling of skeletal muscle is incompletely understood. We hypothesized that high force development during RE is more important for post-exercise-induced signalling than submaximal and...

  • A family of cytokine-inducible inhibitors of signalling. Starr, Robyn; Wilson, Tracy A. // Nature;6/26/1997, Vol. 387 Issue 6636, p917 

    Reports the results of the cloning of a complementary DNA encoding a protein SOCS-1, containing a SH2-domain, by its ability to inhibit the macrophage differentiation of M1 cells in response to interleukin-6. The inhibition of both interleukin-6-induced receptor phosphorylation and the...

  • Signalling polarity. Moon, Randall T.; Shah, Kavita // Nature;5/16/2002, Vol. 417 Issue 6886, p239 

    Focuses on the processes of cell-to-cell signalling. Role of transcription factors in regulating gene expression; Influence of intracellular signalling pathways on cell proliferation, death and behavior; Information on the non-canonical planar cell polarity pathway.

  • Beating a path to Myc. Eisenman, Robert N.; Cooper, Jonathan A. // Nature;11/30/1995, Vol. 378 Issue 6556, p438 

    Discusses the involvement of the transcription factor, Myc, in gene expression. Extracellular growth signals reaching the nucleus; Stimulation of mitogens or cytokines of diverse cell types; Separate signalling pathways for inducing the Myc and fos genes; Interfering form of Myc blocking entry...

  • Gelsolin (GSN) induces cardiomyocyte hypertrophy and BNP expression via p38 signaling and GATA-4 transcriptional factor activation. Hu, Wei-Syun; Ho, Tsung-Jung; Pai, Peiying; Chung, Li-Chin; Kuo, Chia-Hua; Chang, Sheng-Huang; Tsai, Fuu-Jen; Tsai, Chang-Hai; Jie, Yu-Chi; Liou, Ying-Ming; Huang, Chih-Yang // Molecular & Cellular Biochemistry;May2014, Vol. 390 Issue 1/2, p263 

    Cardiomyocyte hypertrophy is an adaptive response of the heart to various types of stress. During the period of stress accumulation, the transition from physiological hypertrophy to pathological hypertrophy results in the promotion of heart failure. Gelsolin (GSN) is a member of the...

  • Downregulation of IL-6-induced STAT3 tyrosine phosphorylation by TGF-beta1 is mediated by caspase-dependent and -independent processes. Wierenga, A.T.J.; Schuringa, J.J.; Eggen, B.J.L.; Kruijer, W.; Vellenga, E. // Leukemia (08876924);Apr2002, Vol. 16 Issue 4, p675 

    To explore the possible cross-talk between the IL-6 and TGF-beta1 pathways in AML blast cells, the effect of TGF-beta1 pretreatment on IL-6-induced STAT3 tyrosine phosphorylation was studied. A reduction of STAT3 tyrosine phosphorylation after TGF-beta1 pretreatment was observed in four out of...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics