Storage and Release of Spermatozoa from the Pre-Uterine Tube Reservoir

Freeman, Sarah L.; England, Gary C.W.
February 2013
PLoS ONE;Feb2013, Vol. 8 Issue 2, p1
Academic Journal
In mammals, after coitus a small number of spermatozoa enter the uterine tube and following attachment to uterine tube epithelium are arrested in a non-capacitated state until peri-ovulatory signalling induces their detachment. Whilst awaiting release low numbers of spermatozoa continually detach from the epithelium and the uterine tube reservoir risks depletion. There is evidence of attachment of spermatozoa to uterine epithelium in several species which might form a potential pre-uterine tube reservoir. In this study we demonstrate that: (1) dog spermatozoa attach to uterine epithelium and maintain flagellar activity, (2) in non-capacitating conditions spermatozoa progressively detach with a variety of motility characteristics, (3) attachment is not influenced by epithelial changes occurring around ovulation, (4) attachment to uterine epithelium slows capacitation, (5) capacitated spermatozoa have reduced ability to attach to uterine epithelium, (6) under capacitating conditions increased numbers of spermatozoa detach and exhibit transitional and hyperactive motility which differ to those seen in non-capacitating conditions, (7) detachment of spermatozoa and motility changes can be induced by post-ovulation but not pre-ovulation uterine tube flush fluid and by components of follicular fluid and solubilised zona pellucida, (8) prolonged culture does not change the nature of the progressive detachment seen in non-capacitating conditions nor the potential for increased detachment in capacitating conditions. We postulate that in some species binding of spermatozoa to uterine epithelium is an important component of the transport of spermatozoa. Before ovulation low numbers of spermatozoa continually detach, including those which are non-capacitated with fast forward progressive motility allowing the re-population of the uterine tube, whilst around the time of ovulation, signalling from as-yet unknown factors associated with follicular fluid, oocytes and uterine tube secretion promotes the detachment of large numbers of capacitated spermatozoa with hyperactive motility that may contribute to the fertilising pool.


Related Articles

  • Connexin43 Expression Increases in the Epithelium and Stroma along the Colonic Neoplastic Progression Pathway: Implications for Its Oncogenic Role. Yusheng Han; Zhang, Paul J.; Chen, Terina; Yum, Sabrina W.; Pasha, Teresa; Furth, Emma E. // Gastroenterology Research & Practice;2011, Vol. 2011, p1 

    Connexins (Cxs) are critical for normal tissue development, differentiation, and cell proliferation. Normal expression and function of Cxs are considered to play a role in tumor suppression, but abnormal localization and abnormally increased expression of Cxs have been found in a variety of...

  • Protein kinase C ζ is a positive modulator of canonical Wnt signaling pathway in tumoral colon cell lines. Luna-Ulloa, Luis Bernardo; Hernández-Maqueda, José G.; Santoyo-Ramos, Paula; Castañeda-Patlán, M.Cristina; Robles-Flores, Martha // Carcinogenesis;Nov2011, Vol. 32 Issue 11, p1615 

    The colonic epithelium is a continuously renewing tissue with a dynamic turnover of cells. Wnt pathway is a key regulator of its homeostasis and is altered in a large proportion of colon cancers. Protein kinase C (PKC) family of serine/threonine kinases are also involved in colon tumor formation...

  • Targeting the FOXO1/KLF6 axis regulates EGFR signaling and treatment response. Sangodkar, Jaya; Dhawan, Neil S.; Melville, Heather; Singh, Varan J.; Yuan, Eric; Rana, Huma; Izadmehr, Sudeh; Farrington, Caroline; Mazhar, Sahar; Katz, Suzanna; Albano, Tara; Arnovitz, Pearlann; Okrent, Rachel; Ohlmeyer, Michael; Galsky, Matthew; Burstein, David; Zhang, David; Politi, Katerina; DiFeo, Analisa; Narla, Goutham // Journal of Clinical Investigation;Jul2012, Vol. 122 Issue 7, p2637 

    EGFR activation is both a key molecular driver of disease progression and the target of a broad class of molecular agents designed to treat advanced cancer. Nevertheless, resistance develops through several mechanisms, including activation of AKT signaling. Though much is known about the...

  • In vitro assessment of some sperm function following exposure to levonorgestrel in human fallopian tubes.  // Reproductive Biology & Endocrinology;2012, Vol. 10 Issue 1, p8 

    The article presents a study focusing on in vitro assessment of some sperm function following exposure to levonorgestrel (LNG) in human fallopian tubes. The study shows that LNG had no effect on the number of motile spermatozoa recovered from the human fallopian tubes in vitro, on their adhesion...

  • The effects of oviductal cell co-culture and different gas mixtures on the development of bovine embryos in vitro. Özdaş, Özen Banu; Cirit, Ümüt; Baran, Alper; Kaşikçi, Güven // Turkish Journal of Veterinary & Animal Sciences;Aug2012, Vol. 36 Issue 4, p362 

    The objective of the current study was to investigate the effects of the addition of oviduct co-culture to synthetic oviduct fluid (SOF) and M´n´zo B2 media used in an in vitro study of cows, and the effects of different gas atmospheres (5% CO2 or 5% CO2, 5% O2, and 90% N2) on in vitro...

  • α-Enolase (ENO1) Inhibits Epithelial-mesenchymal Transition in the A549 Cell Line by Suppressing ERK1/2 Phosphorylation. Xin ZHOU; Ying ZHANG; Naijun HAN; Suping GUO; Ting XIAO; Shujun CHENG; Yanning GAO; Kaitai ZHANG // Chinese Journal of Lung Cancer;May2013, Vol. 16 Issue 5, p221 

    Background and objective It has been proven that epithelial-mesenchymal transition (EMT) is a critical process which is precisely regulated by multiple signaling pathways during the progression and metastasis of non-small cell lung cancer (NSCLC). Canonical MAPK signaling is essential to...

  • Nitric Oxide is a Signaling Molecule in Spermatozoa. Herrero, Maria Belen; de Lamirande, Eve; Gagnon, Claude // Current Pharmaceutical Design;Feb2003, Vol. 9 Issue 5, p419 

    Low and controlled concentrations of nitric oxide play an important role in sperm physiology. Nitric oxide is produced by spermatozoa and acts as an intracellular signaling molecule in the processes of capacitation and acrosome reaction. It has been documented that during capacitation, nitric...

  • Platelet activating factor increases ciliary activity in the hamster oviduct through epithelial production of prostaglandin E2. Hermoso, M.; Barrera, N.; Morales, B.; Pérez, S.; Villalón, M. // Pflugers Archiv European Journal of Physiology;Jun2001, Vol. 442 Issue 3, p336 

    We investigated the signal transduction mechanisms associated with an increase in ciliary beat frequency (CBF) produced by platelet activating factor (PAF) in oviductal ciliated cell cultures. In the range of concentrations similar to that produced by preimplantation embryos, PAF increased the...

  • Enhanced beta-catenin expression and inflammation are associated with human ectopic tubal pregnancy. Li, Ping; Zhu, Wei-jie; Ma, Zheng-lai; Wang, Guang; Peng, Hui; Chen, Yao; Lee, Kenneth Ka Ho; Yang, Xuesong // Human Reproduction;Sep2013, Vol. 28 Issue 9, p2363 

    STUDY QUESTION Is there a molecular link between Wnt signaling in fallopian tube inflammation and ectopic tubal implantation? SUMMARY ANSWER Enhanced beta-catenin expression, reduced E-cadherin expression and glycogen accumulation in the tubal epithelia and hyperplasia in tubal arteries were...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics