The SWI/SNF Chromatin Remodeling Complex Influences Transcription by RNA Polymerase I in Saccharomyces cerevisiae

Zhang, Yinfeng; Anderson, Susan J.; French, Sarah L.; Sikes, Martha L.; Viktorovskaya, Olga V.; Huband, Jacalyn; Holcomb, Katherine; Hartman IV, John L.; Beyer, Ann L.; Schneider, David A.
February 2013
PLoS ONE;Feb2013, Vol. 8 Issue 2, p1
Academic Journal
SWI/SNF is a chromatin remodeling complex that affects transcription initiation and elongation by RNA polymerase II. Here we report that SWI/SNF also plays a role in transcription by RNA polymerase I (Pol I) in Saccharomyces cerevisiae. Deletion of the genes encoding the Snf6p or Snf5p subunits of SWI/SNF was lethal in combination with mutations that impair Pol I transcription initiation and elongation. SWI/SNF physically associated with ribosomal DNA (rDNA) within the coding region, with an apparent peak near the 5′ end of the gene. In snf6Δ cells there was a ∼2.5-fold reduction in rRNA synthesis rate compared to WT, but there was no change in average polymerase occupancy per gene, the number of rDNA gene repeats, or the percentage of transcriptionally active rDNA genes. However, both ChIP and EM analyses showed a small but reproducible increase in Pol I density in a region near the 5′ end of the gene. Based on these data, we conclude that SWI/SNF plays a positive role in Pol I transcription, potentially by modifying chromatin structure in the rDNA repeats. Our findings demonstrate that SWI/SNF influences the most robust transcription machinery in proliferating cells.


Related Articles

  • The Transition of Poised RNA Polymerase II to an Actively Elongating State Is a "Complex" Affair. Yearling, Marie N.; Radebaugh, Catherine A.; Stargell, Laurie A. // Genetics Research International;2011, p1 

    The initial discovery of the occupancy of RNA polymerase II at certain genes prior to their transcriptional activation occurred a quarter century ago in Drosophila. The preloading of these poised complexes in this inactive state is now apparent in many different organisms across the evolutionary...

  • Mutant Versions of the S. cerevisiae Transcription Elongation Factor Spt16 Define Regions of Spt16 That Functionally Interact with Histone H3. Myers, Catherine N.; Berner, Gary B.; Holthoff, Joseph H.; Martinez-Fonts, Kirby; Harper, Jennifer A.; Alford, Sarah; Taylor, Megan N.; Duina, Andrea A. // PLoS ONE;2011, Vol. 6 Issue 6, p1 

    In eukaryotic cells, the highly conserved FACT (FAcilitates Chromatin Transcription) complex plays important roles in several chromatin-based processes including transcription initiation and elongation. During transcription elongation, the FACT complex interacts directly with nucleosomes to...

  • The Prefoldin Complex Regulates Chromatin Dynamics during Transcription Elongation. Millán-Zambrano, Gonzalo; Rodríguez-Gil, Alfonso; Peñate, Xenia; de Miguel-Jiménez, Lola; Morillo-Huesca, Macarena; Krogan, Nevan; Chávez, Sebastián // PLoS Genetics;Sep2013, Vol. 9 Issue 9, p1 

    Transcriptional elongation requires the concerted action of several factors that allow RNA polymerase II to advance through chromatin in a highly processive manner. In order to identify novel elongation factors, we performed systematic yeast genetic screening based on the GLAM (Gene...

  • Spatial Organization and Dynamics of Transcription Elongation and Pre-mRNA Processing in Live Cells. Sánchez-Álvarez, Miguel; Sánchez-Hernández, Noemí; Suñé, Carlos // Genetics Research International;2011, p1 

    During the last 30 years, systematic biochemical and functional studies have significantly expanded our knowledge of the transcriptional molecular components and the pre-mRNA processing machinery of the cell. However, our current understanding of how these functions take place spatiotemporally...

  • Transcriptional repression of the yeast CHA1 gene requires the chromatin-remodeling complex RSC. Moreira, Jose M. A.; Holmberg, Steen // EMBO Journal;5/17/99, Vol. 18 Issue 10, p2836 

    In eukaryotes, DNA is packaged into chromatin, a compact structure that must be disrupted when genes are transcribed by RNA polymerase II. For transcription to take place, chromatin is remodeled via nucleosome disruption or displacement, a fundamental transcriptional regulatory mechanism in...

  • CTR9, a Component of PAF Complex, Controls Elongation Block at the c-Fos Locus via Signal-Dependent Regulation of Chromatin-Bound NELF Dissociation. Yoo, Hyun-Seok; Seo, Jung-Hwa; Yoo, Joo-Yeon // PLoS ONE;Apr2013, Vol. 8 Issue 4, p1 

    PAF complex (PAFc) is an RNA polymerase II associated factor that controls diverse steps of transcription. Although it is generally associated with actively transcribed genes, a repressive PAFc has also been suggested. Here, we report that PAFc regulates the transition from transcription...

  • Intergenic transcription is required to repress the Saccharomyces cerevisiae SER3 gene. Martens, Joseph A.; Laprade, Lisa; Winston, Fred // Nature;6/3/2004, Vol. 429 Issue 6991, p571 

    Transcription by RNA polymerase II in Saccharomyces cerevisiae and in humans is widespread, even in genomic regions that do not encode proteins. The purpose of such intergenic transcription is largely unknown, although it can be regulatory. We have discovered a role for one case of intergenic...

  • Transcript assisted phosphodiester bond hydrolysis by eukaryotic RNA polymerase II. Nielsen, Soren; Zenkin, Nikolay // Transcription (2154-1264);Nov2013, Vol. 5, p1 

    The authors discuss the transcript assisted hydrolysis which is involved in RNA cleavage through eukaryotic RNA polymerase II. They also discuss multisubunit RNA polymerase (RNAP) enzyme that accomplishes transcription in all living organisms, discovery that a nucleotide monophosphate (NMP) at...

  • The super elongation complex (SEC) family in transcriptional control. Luo, Zhuojuan; Lin, Chengqi; Shilatifard, Ali // Nature Reviews Molecular Cell Biology;Sep2012, Vol. 13 Issue 9, p543 

    The super elongation complex (SEC) consists of the RNA polymerase II (Pol II) elongation factors eleven-nineteen Lys-rich leukaemia (ELL) proteins, positive transcription elongation factor b (P-TEFb) and several frequent mixed lineage leukaemia (MLL) translocation partners. It is one of the most...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics