TITLE

Electroacupuncture Acutely Improves Cerebral Blood Flow and Attenuates Moderate Ischemic Injury via an Endothelial Mechanism in Mice

AUTHOR(S)
Kim, Ji Hyun; Choi, Kyung Ha; Jang, Young Jung; Bae, Sun Sik; Shin, Byung-Cheul; Choi, Byung Tae; Shin, Hwa Kyoung
PUB. DATE
February 2013
SOURCE
PLoS ONE;Feb2013, Vol. 8 Issue 2, p1
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Electroacupuncture (EA) is a novel therapy based on traditional acupuncture combined with modern eletrotherapy that is currently being investigated as a treatment for acute ischemic stroke. Here, we studied whether acute EA stimulation improves tissue and functional outcome following experimentally induced cerebral ischemia in mice. We hypothesized that endothelial nitric oxide synthase (eNOS)-mediated perfusion augmentation was related to the beneficial effects of EA by interventions in acute ischemic injury. EA stimulation at Baihui (GV20) and Dazhui (GV14) increased cerebral perfusion in the cerebral cortex, which was suppressed in eNOS KO, but there was no mean arterial blood pressure (MABP) response. The increased perfusion elicited by EA were completely abolished by a muscarinic acetylcholine receptor (mAChR) blocker (atropine), but not a β-adrenergic receptor blocker (propranolol), an α-adrenergic receptor blocker (phentolamine), or a nicotinic acetylcholine receptor (nAChR) blocker (mecamylamine). In addition, EA increased acetylcholine (ACh) release and mAChR M3 expression in the cerebral cortex. Acute EA stimulation after occlusion significantly reduced infarct volume by 34.5% when compared to a control group of mice at 24 h after 60 min-middle cerebral artery occlusion (MCAO) (moderate ischemic injury), but not 90-min MCAO (severe ischemic injury). Furthermore, the impact of EA on moderate ischemic injury was totally abolished in eNOS KO. Consistent with a smaller infarct size, acute EA stimulation led to prominent improvement of neurological function and vestibule-motor function. Our results suggest that acute EA stimulation after moderate focal cerebral ischemia, but not severe ischemia improves tissue and functional recovery and ACh/eNOS-mediated perfusion augmentation might be related to these beneficial effects of EA by interventions in acute ischemic injury.
ACCESSION #
87624880

 

Related Articles

  • Vascular permeability in cerebral cavernous malformations. Mikati, Abdul G; Khanna, Omaditya; Zhang, Lingjiao; Girard, Romuald; Shenkar, Robert; Guo, Xiaodong; Shah, Akash; Larsson, Henrik BW; Tan, Huan; Li, Luying; Wishnoff, Matthew S; Shi, Changbin; Christoforidis, Gregory A; Awad, Issam A // Journal of Cerebral Blood Flow & Metabolism;Oct2015, Vol. 35 Issue 10, p1632 

    Patients with the familial form of cerebral cavernous malformations (CCMs) are haploinsufficient for the CCM1, CCM2, or CCM3 gene. Loss of corresponding CCM proteins increases RhoA kinase-mediated endothelial permeability in vitro, and in mouse brains in vivo. A prospective case-controlled...

  • Intravenous immunoglobulin (IVIg) provides protection against endothelial cell dysfunction and death in ischemic stroke. Widiapradja, Alexander; Santro, Tomislav; Basta, Milan; Sobey, Christopher G.; Manzanero, Silvia; Arumugam, Thiruma V. // Experimental & Translational Stroke Medicine;2014, Vol. 6 Issue 1, p1 

    Background: The brain endothelium is a key component of the blood brain barrier which is compromised following ischemia, allowing infiltration of damaging immune cells and other inflammatory molecules into the brain. Intravenous immunoglobulin (IVIg) is known to reduce infarct size in a mouse...

  • Effect of Electroacupuncture on Bronchial Asthma Induced by Ovalbumin in Rats. Carneiro, Elisabete Regis; Carneiro, Celia Regina Whitaker; De Castro, Marie Alice Pedreira; Yamamura, Ysao; Flor Silveira, Vera Lucia // Journal of Alternative & Complementary Medicine;Feb2005, Vol. 11 Issue 1, p127 

    Asthma is a worldwide disabling chronic inflammatory airway disease characterized by an intense eosinophilic inflammatory infiltrate on bronchial mucous membranes. Among the complementary therapeutic approaches to asthma, acupuncture has been widely used.Here we used a rat pulmonary...

  • Elevated Level of Fibrinogen Increases Caveolae Formation; Role of Matrix Metalloproteinase-9. Muradashvili, Nino; Benton, Richard; Tyagi, Reeta; Tyagi, Suresh; Lominadze, David // Cell Biochemistry & Biophysics;Jun2014, Vol. 69 Issue 2, p283 

    The role of the inflammatory agent fibrinogen (Fg) in increased pial venular permeability has been shown previously. It was suggested that an activation of matrix metalloproteinase-9 (MMP-9) is involved in Fg-induced enhanced transcytosis through endothelial cells (ECs). However, direct link...

  • Endothelial Nitric Oxide Synthase Reduces Crescentic and Necrotic Glomerular Lesions, Reactive Oxygen Production, and MCP1 Production in Murine Lupus Nephritis Gilkeson, Gary S.; Mashmoushi, Ahmad K.; Ruiz, Phillip; Caza, Tiffany N.; Perl, Andras; Oates, Jim C. // PLoS ONE;May2013, Vol. 8 Issue 5, p1 

    Systemic lupus erythematosus, in both animal models and in humans, is characterized by autoantibody production followed by immune complex deposition in target tissues. Ensuing target organ damage is modulated by reactive intermediates, including reactive nitrogen and oxygen species, through as...

  • Intravenous tPA Therapy Does Not Worsen Acute Intracerebral Hemorrhage in Mice. Foerch, Christian; Rosidi, Nathanael L.; Schlunk, Frieder; Lauer, Arne; Cianchetti, Flor A.; Mandeville, Emiri; Arai, Ken; Yigitkanli, Kazim; Fan, Xiang; Wang, Xiaoying; van Leyen, Klaus; Steinmetz, Helmuth; Schaffer, Chris B.; Lo, Eng H. // PLoS ONE;Feb2013, Vol. 8 Issue 2, p1 

    Tissue plasminogen activator (tPA) is the only FDA-approved treatment for reperfusing ischemic strokes. But widespread use of tPA is still limited by fears of inadvertently administering tPA in patients with intracerebral hemorrhage (ICH). Surprisingly, however, the assumption that tPA will...

  • Decreased Amphetamine-Induced Locomotion and Improved Latent Inhibition in Mice Mutant for the MS Muscarinic Receptor Gene Found in the Human 15q Schizophrenia Region. Haoran Wang; Ng, Karen; Hayes, David; Xiangrong Gao; Forster, Gina; Blaha, Charles; Yeomans, John // Neuropsychopharmacology;Dec2004, Vol. 29 Issue 12, p2126 

    MS muscarinic receptors are coexpressed with D2 dopamine receptors in the ventral tegmentum and striatum, and are important for reward in rodents. Previously, we reported that disruption of the MS receptor gene in mice reduced dopamine release in the nucleus accumbens. In this study, we...

  • Differential Roles of α6β2* and α4β2* Neuronal Nicotinic Receptors in Nicotine- and Cocaine-Conditioned Reward in Mice. Sanjakdar, Sarah S; Maldoon, Pretal P; Marks, Michael J; Brunzell, Darlene H; Maskos, Uwe; McIntosh, J Michael; Bowers, M Scott; Damaj, M Imad // Neuropsychopharmacology;Jan2015, Vol. 40 Issue 2, p350 

    Mesolimbic α6* nicotinic acetylcholine receptors (nAChRs) are thought to have an important role in nicotine behavioral effects. However, little is known about the role of the various α6*-nAChRs subtypes in the rewarding effects of nicotine. In this report, we investigated and compared the...

  • Neurogenesis: Support from the cortex. Carr, Fiona // Nature Reviews Neuroscience;May2015, Vol. 16 Issue 5, p248 

    The article discusses a research concerning the major role of pericytes and endothelial cells from the mouse ventricular-subventricular zone (V-SVZ) to promoted proliferation and differentiation of neural stem cells in vitro.

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics