TITLE

Global and Stage Specific Patterns of Krüppel-Associated-Box Zinc Finger Protein Gene Expression in Murine Early Embryonic Cells

AUTHOR(S)
Corsinotti, Andrea; Kapopoulou, Adamandia; Gubelmann, Carine; Imbeault, Michael; Santoni de Sio, Francesca R.; Rowe, Helen M.; Mouscaz, Yoann; Deplancke, Bart; Trono, Didier
PUB. DATE
February 2013
SOURCE
PLoS ONE;Feb2013, Vol. 8 Issue 2, p1
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Highly coordinated transcription networks orchestrate the self-renewal of pluripotent stem cell and the earliest steps of mammalian development. KRAB-containing zinc finger proteins represent the largest group of transcription factors encoded by the genomes of higher vertebrates including mice and humans. Together with their putatively universal cofactor KAP1, they have been implicated in events as diverse as the silencing of endogenous retroelements, the maintenance of imprinting and the pluripotent self-renewal of embryonic stem cells, although the genomic targets and specific functions of individual members of this gene family remain largely undefined. Here, we first generated a list of Ensembl-annotated KRAB-containing genes encoding the mouse and human genomes. We then defined the transcription levels of these genes in murine early embryonic cells. We found that the majority of KRAB-ZFP genes are expressed in mouse pluripotent stem cells and other early progenitors. However, we also identified distinctively cell- or stage-specific patterns of expression, some of which are pluripotency-restricted. Finally, we determined that individual KRAB-ZFP genes exhibit highly distinctive modes of expression, even when grouped in genomic clusters, and that these cannot be correlated with the presence of prototypic repressive or activating chromatin marks. These results pave the way to delineating the role of specific KRAB-ZFPs in early embryogenesis.
ACCESSION #
87624866

 

Related Articles

  • Zscan4 promotes genomic stability during reprogramming and dramatically improves the quality of iPS cells as demonstrated by tetraploid complementation. Jiang, Jing; Lv, Wenjian; Ye, Xiaoying; Wang, Lingbo; Zhang, Man; Yang, Hui; Okuka, Maja; Zhou, Chikai; Zhang, Xuan; Liu, Lin; Li, Jinsong // Cell Research;Jan2013, Vol. 23 Issue 1, p92 

    Induced pluripotent stem (iPS) cells generated using Yamanaka factors have great potential for use in autologous cell therapy. However, genomic abnormalities exist in human iPS cells, and most mouse iPS cells are not fully pluripotent, as evaluated by the tetraploid complementation assay (TCA);...

  • Kidney Specific Protein-Positive Cells Derived from Embryonic Stem Cells Reproduce Tubular Structures In Vitro and Differentiate into Renal Tubular Cells. Morizane, Ryuji; Monkawa, Toshiaki; Fujii, Shizuka; Yamaguchi, Shintaro; Homma, Koichiro; Matsuzaki, Yumi; Okano, Hideyuki; Itoh, Hiroshi // PLoS ONE;Jun2013, Vol. 8 Issue 6, p1 

    Embryonic stem cells and induced pluripotent stem cells have the ability to differentiate into various organs and tissues, and are regarded as new tools for the elucidation of disease mechanisms as well as sources for regenerative therapies. However, a method of inducing organ-specific cells...

  • Advances in Culture and Manipulation of Human Pluripotent Stem Cells. Qian, X.; Villa-Diaz, L.G.; Krebsbach, P.H. // Journal of Dental Research;Nov2013, Vol. 92 Issue 11, p956 

    Recent advances in the understanding of pluripotent stem cell biology and emerging technologies to reprogram somatic cells to a stem cell–like state are helping bring stem cell therapies for a range of human disorders closer to clinical reality. Human pluripotent stem cells (hPSCs) have...

  • Rapid and Efficient Generation of Functional Motor Neurons From Human Pluripotent Stem Cells Using Gene Delivered Transcription Factor Codes. Hester, Mark E; Murtha, Matthew J; Song, SungWon; Rao, Meghan; Miranda, Carlos J; Meyer, Kathrin; Tian, Jinbin; Boulting, Gabriella; Schaffer, David V; Zhu, Michael X; Pfaff, Samuel L; Gage, Fred H; Kaspar, Brian K // Molecular Therapy;Oct2011, Vol. 19 Issue 10, p1905 

    Stem cell-derived motor neurons (MNs) are increasingly utilized for modeling disease in vitro and for developing cellular replacement strategies for spinal cord injury and diseases such as spinal muscular atrophy (SMA) and amyotrophic lateral sclerosis (ALS). Human embryonic stem cell (hESC)...

  • Zfp296 Is a Novel, Pluripotent-Specific Reprogramming Factor. Fischedick, Gerrit; Klein, Diana C.; Wu, Guangming; Esch, Daniel; Höing, Susanne; Han, Dong Wook; Reinhardt, Peter; Hergarten, Kerstin; Tapia, Natalia; Schöler, Hans R.; Sterneckert, Jared L. // PLoS ONE;Apr2012, Vol. 7 Issue 4, p1 

    Expression of the four transcription factors Oct4, Sox2, Klf4, and c-Myc (OSKM) is sufficient to reprogram somatic cells into induced pluripotent stem (iPSCs). However, this process is slow and inefficient compared with the fusion of somatic cells with embryonic stem cells (ESCs), indicating...

  • CRISPR Reveals a Distal Super-Enhancer Required for Sox2 Expression in Mouse Embryonic Stem Cells. Li, Yan; Rivera, Chloe M.; Ishii, Haruhiko; Jin, Fulai; Selvaraj, Siddarth; Lee, Ah Young; Dixon, Jesse R.; Ren, Bing // PLoS ONE;Dec2014, Vol. 9 Issue 12, p1 

    The pluripotency of embryonic stem cells (ESCs) is maintained by a small group of master transcription factors including Oct4, Sox2 and Nanog. These core factors form a regulatory circuit controlling the transcription of a number of pluripotency factors including themselves. Although previous...

  • A central role for TFIID in the pluripotent transcription circuitry. Pijnappel, W. W. M. Pim; Esch, Daniel; Baltissen, Marijke P. A.; Wu, Guangming; Mischerikow, Nikolai; Bergsma, Atze J.; van der Wal, Erik; Han, Dong Wook; Bruch, Hermann vom; Moritz, Sören; Lijnzaad, Phillip; Altelaar, A. F. Maarten; Sameith, Katrin; Zaehres, Holm; Heck, Albert J. R.; Holstege, Frank C. P.; Schöler, Hans R.; Timmers, H. T. Marc // Nature;3/28/2013, Vol. 495 Issue 7442, p516 

    Embryonic stem (ES) cells are pluripotent and characterized by open chromatin and high transcription levels, achieved through auto-regulatory and feed-forward transcription factor loops. ES-cell identity is maintained by a core of factors including Oct4 (also known as Pou5f1), Sox2, Klf4, c-Myc...

  • Epigenetics of induced pluripotency, the seven-headed dragon. Djuric, Ugljesa; Ellis, James // Stem Cell Research & Therapy;2010, Vol. 1 Issue 1, p1 

    Induction of pluripotency from somatic cells by exogenous transcription factors is made possible by a variety of epigenetic changes that take place during the reprogramming process. The derivation of fully reprogrammed induced pluripotent stem (iPS) cells is achieved through establishment of...

  • Embryonic stem cell and induced pluripotent stem cell: an epigenetic perspective. Liang, Gaoyang; Zhang, Yi // Cell Research;Jan2013, Vol. 23 Issue 1, p49 

    Pluripotent stem cells, like embryonic stem cells (ESCs), have specialized epigenetic landscapes, which are important for pluripotency maintenance. Transcription factor-mediated generation of induced pluripotent stem cells (iPSCs) requires global change of somatic cell epigenetic status into an...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics