The h-Current in Periglomerular Dopaminergic Neurons of the Mouse Olfactory Bulb

Pignatelli, Angela; Borin, Mirta; Fogli Iseppe, Alex; Gambardella, Cristina; Belluzzi, Ottorino
February 2013
PLoS ONE;Feb2013, Vol. 8 Issue 2, p1
Academic Journal
The properties of the hyperpolarization-activated cation current (Ih) were investigated in rat periglomerular dopaminergic neurons using patch-clamp recordings in thin slices. A reliable identification of single dopaminergic neurons was made possible by use of a transgenic line of mice expressing eGFP under the tyrosine hydroxylase promoter. At 37 °C and minimizing the disturbance of the intracellular milieu with perforated patches, this current shows a midpoint of activation around −82.7 mV, with a significant level of opening already at rest, thereby giving a substantial contribution to the resting potential, and ultimately playing a relevant function in the control of the cell excitability. The blockage of Ih has a profound influence on the spontaneous firing of these neurons, which result as strongly depressed. However the effect is not due to a direct role of the current in the pacemaker process, but to the Ih influence on the resting membrane potential. Ih kinetics is sensitive to the intracellular levels of cAMP, whose increase promotes a shift of the activation curve towards more positive potentials. The direct application of DA and 5-HT neurotransmitters, physiologically released onto bulbar dopaminergic neurons and known to act on metabotropic receptors coupled to the cAMP pathway, do not modifythe Ih amplitude. On the contrary, noradrenaline almost halves the Ih amplitude. Our data indicate that the HCN channels do not participate directly to the pacemaker activity of periglomerular dopaminergic neurons, but influence their resting membrane potential by controlling the excitability profile of these cells, and possibly affecting the processing of sensory information taking place at the entry of the bulbar circuitry.


Related Articles

  • Female mice lacking cholecystokinin 1 receptors have compromised neurogenesis, and fewer dopaminergic cells in the olfactory bulb. Yi Sui; Vermeulen, Rob; Hökfelt, Tomas; Horne, Malcolm K.; Stanić, Davor // Frontiers in Cellular Neuroscience;Mar2013, Vol. 7, p1 

    Neurogenesis in the adult rodent brain is largely restricted to the subependymal zone (SVZ) of the lateral ventricle and subgranular zone (SGZ) of the dentate gyrus (DG). We examined whether cholecystokinin (CCK) through actions mediated by CCK1 receptors (CCK1R) is involved in regulating...

  • Dose and Time-Dependent Selective Neurotoxicity Induced by Mephedrone in Mice. Martínez-Clemente, José; López-Arnau, Raúl; Abad, Sonia; Pubill, David; Escubedo, Elena; Camarasa, Jorge // PLoS ONE;Jun2014, Vol. 9 Issue 6, p1 

    Mephedrone is a drug of abuse marketed as ‘bath salts". There are discrepancies concerning its long-term effects. We have investigated the neurotoxicity of mephedrone in mice following different exposition schedules. Schedule 1: four doses of 50 mg/kg. Schedule 2: four doses of 25 mg/kg....

  • Expression of the transcription factor regulatory factor X1 in the mouse brain. Feng, Chenzhuo; Li, Jiejie; Zuo, Zhiyi // Folia Histochemica et Cytobiologica;2011, Vol. 49 Issue 2, p344 

    Abstract: Limited information indicates that the regulatory factor X1 (RFX1), the prototype member of the transcription factor RFX family, may play a role in the central nervous system. Our recent study showed that knockout of the Rfx1 gene led to early embryonic death. In the present study, we...

  • Local corticotropin releasing hormone (CRH) signals to its receptor CRHR1 during postnatal development of the mouse olfactory bulb. Garcia, Isabella; Bhullar, Paramjit; Tepe, Burak; Ortiz-Guzman, Joshua; Huang, Longwen; Herman, Alexander; Chaboub, Lesley; Deneen, Benjamin; Justice, Nicholas; Arenkiel, Benjamin // Brain Structure & Function;Jan2016, Vol. 221 Issue 1, p1 

    Neuropeptides play important physiological functions during distinct behaviors such as arousal, learning, memory, and reproduction. However, the role of local, extrahypothalamic neuropeptide signaling in shaping synapse formation and neuronal plasticity in the brain is not well understood. Here,...

  • Neuropeptide S Facilitates Mice Olfactory Function through Activation of Cognate Receptor-Expressing Neurons in the Olfactory Cortex. Shao, Yu-Feng; Zhao, Peng; Dong, Chao-Yu; Li, Jing; Kong, Xiang-Pan; Wang, Hai-Liang; Dai, Li-Rong; Hou, Yi-Ping // PLoS ONE;Apr2013, Vol. 8 Issue 4, p1 

    Neuropeptide S (NPS) is a newly identified neuromodulator located in the brainstem and regulates various biological functions by selectively activating the NPS receptors (NPSR). High level expression of NPSR mRNA in the olfactory cortex suggests that NPS-NPSR system might be involved in the...

  • miR-7a regulation of Pax6 controls spatial origin of forebrain dopaminergic neurons. de Chevigny, Antoine; Coré, Nathalie; Follert, Philipp; Gaudin, Marion; Barbry, Pascal; Béclin, Christophe; Cremer, Harold // Nature Neuroscience;Aug2012, Vol. 15 Issue 8, p1120 

    In the postnatal and adult mouse forebrain, a mosaic of spatially separated neural stem cells along the lateral wall of the ventricles generates defined types of olfactory bulb neurons. To understand the mechanisms underlying the regionalization of the stem cell pool, we focused on the...

  • Regenerative medicine: Bespoke cells for the human brain. Sendtner, Michael // Nature;8/11/2011, Vol. 476 Issue 7359, p158 

    The article presents several studies which describe the direct conversion of neurons from human skin fibroblast cells. It states that Z. P. Pang and colleagues' study shows that the efficacy of human fibroblast conversion into neurons is developed through an extra transcription factor NeuroD1....

  • Decreased expression of organic cation transporters, Oct1 and Oct2, in brain microvessels and its implication to MPTP-induced dopaminergic toxicity in aged mice. Wu, Kuo-Chen; Lu, Ya-Hsuan; Peng, Yi-Hsuan; Tsai, Ting-Fen; Kao, Yu-Han; Yang, Hui-Ting; Lin, Chun-Jung // Journal of Cerebral Blood Flow & Metabolism;Jan2015, Vol. 35 Issue 1, p37 

    This study was to investigate the influence of age on the expression of organic cation transporters (OCTs) that belong to the SLC22 family in brain microvessels (BMVs) and its implications for 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced dopaminergic toxicity in mice. Here, we...

  • Increased Ndfip1 in the Substantia Nigra of Parkinsonian Brains Is Associated with Elevated Iron Levels. Howitt, Jason; Gysbers, Amanda M.; Ayton, Scott; Carew-Jones, Francine; Putz, Ulrich; Finkelstein, David I.; Halliday, Glenda M.; Tan, Seong-Seng // PLoS ONE;Jan2014, Vol. 9 Issue 1, p1 

    Iron misregulation is a central component in the neuropathology of Parkinson's disease. The iron transport protein DMT1 is known to be increased in Parkinson's brains linking functional transport mechanisms with iron accumulation. The regulation of DMT1 is therefore critical to the management of...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics