TITLE

Significant Molecular and Systemic Adaptations after Repeated Sprint Training in Hypoxia

AUTHOR(S)
Faiss, Raphael; Léger, Bertrand; Vesin, Jean-Marc; Fournier, Pierre-Etienne; Eggel, Yan; Dériaz, Olivier; Millet, Grégoire P.
PUB. DATE
February 2013
SOURCE
PLoS ONE;Feb2013, Vol. 8 Issue 2, p1
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
While intermittent hypoxic training (IHT) has been reported to evoke cellular responses via hypoxia inducible factors (HIFs) but without substantial performance benefits in endurance athletes, we hypothesized that repeated sprint training in hypoxia could enhance repeated sprint ability (RSA) performed in normoxia via improved glycolysis and O2 utilization. 40 trained subjects completed 8 cycling repeated sprint sessions in hypoxia (RSH, 3000 m) or normoxia (RSN, 485 m). Before (Pre-) and after (Post-) training, muscular levels of selected mRNAs were analyzed from resting muscle biopsies and RSA tested until exhaustion (10-s sprint, work-to-rest ratio 1∶2) with muscle perfusion assessed by near-infrared spectroscopy. From Pre- to Post-, the average power output of all sprints in RSA was increased (p<0.01) to the same extent (6% vs 7%, NS) in RSH and in RSN but the number of sprints to exhaustion was increased in RSH (9.4±4.8 vs. 13.0±6.2 sprints, p<0.01) but not in RSN (9.3±4.2 vs. 8.9±3.5). mRNA concentrations of HIF-1α (+55%), carbonic anhydrase III (+35%) and monocarboxylate transporter-4 (+20%) were augmented (p<0.05) whereas mitochondrial transcription factor A (−40%), peroxisome proliferator-activated receptor gamma coactivator 1α (−23%) and monocarboxylate transporter-1 (−36%) were decreased (p<0.01) in RSH only. Besides, the changes in total hemoglobin variations (Δ[tHb]) during sprints throughout RSA test increased to a greater extent (p<0.01) in RSH. Our findings show larger improvement in repeated sprint performance in RSH than in RSN with significant molecular adaptations and larger blood perfusion variations in active muscles.
ACCESSION #
87624687

 

Related Articles

  • FoxO3A promotes metabolic adaptation to hypoxia by antagonizing Myc function. Jensen, Kim Steen; Binderup, Tina; Jensen, Klaus Thorleif; Therkelsen, Ib; Borup, Rehannah; Nilsson, Elise; Multhaupt, Hinke; Bouchard, Caroline; Quistorff, Bjørn; Kjær, Andreas; Landberg, Göran; Staller, Peter // EMBO Journal;11/16/2011, Vol. 30 Issue 22, p4554 

    Exposure of metazoan organisms to hypoxia engages a metabolic switch orchestrated by the hypoxia-inducible factor 1 (HIF-1). HIF-1 mediates induction of glycolysis and active repression of mitochondrial respiration that reduces oxygen consumption and inhibits the production of potentially...

  • Hypoxia-inducible transcription factor-1α promotes hypoxia-induced A549 apoptosis via a mechanism that involves the glycolysis pathway. FengMing Luo; XiaoJing Liu; NaiHong Yan; ShuangQing Li; GuiQun Cao; QingYing Cheng; QingJie Xia; HongJing Wang // BMC Cancer;2006, Vol. 6, p26 

    Background: Hypoxia-inducible transcription factor-1α (HIF-1α), which plays an important role in controlling the hypoxia-induced glycolysis pathway, is a "master" gene in the tissue hypoxia response during tumor development. However, its role in the apoptosis of non-small cell lung cancer...

  • Astrocyte - neuron lactate shuttle may boost more ATP supply to the neuron under hypoxic conditions - in silico study supported by in vitro expression data.  // BMC Systems Biology;2011, Vol. 5 Issue 1, p162 

    The article focuses on a study which aims at analyzing the Astrocyte-Neuron Lactate Shuttle Hypothesis (ANLSH) model in fulfilling energy demand during hypoxia. It mentions the importance of neuro-glial interactions in maintaining brain energy metabolism and explains the ANLSH in which...

  • Hypoxia-regulated microRNA-210 modulates mitochondrial function and decreases ISCU and COX10 expression. Chen, Z.; Li, Y.; Zhang, H.; Huang, P.; Luthra, R. // Oncogene;7/29/2010, Vol. 29 Issue 30, p4362 

    The mechanisms of compromised mitochondrial function under various pathological conditions, including hypoxia, remain largely unknown. Recent studies have shown that microRNA-210 (miR-210) is induced by hypoxia under the regulation of hypoxia-inducible factor-1α and has an important role in...

  • Hypoxic Regulation of Metastasis via Hypoxia-Inducible Factors. Vooijs, Marc A.; Gort, Eelke H.; Groot, Arjan J.; van der Wall, Elsken; van Diest, Paul J. // Current Molecular Medicine;Feb2008, Vol. 8 Issue 1, p60 

    Metastases formation is a major factor in disease progression and accounts for the majority of cancer deaths. The molecular mechanisms controlling invasion, dissemination to blood or lymphatic systems and spread of tumor cells to distant organs are still poorly understood. Recent observations...

  • Human CIA30 is involved in the early assembly of mitochondrial complex I and mutations in its gene cause disease. Dunning, C. J. R.; McKenzie, M.; Sugiana, C.; Lazarou, M.; Silke, J.; Connelly, A.; Fletcher, J. M.; Kirby, D. M.; Thorburn, D. R.; Ryan, M. T. // EMBO Journal;7/11/2007, Vol. 26 Issue 13, p3227 

    In humans, complex I of the respiratory chain is composed of seven mitochondrial DNA (mtDNA)-encoded and 38 nuclear-encoded subunits that assemble together in a process that is poorly defined. To date, only two complex I assembly factors have been identified and how each functions is not clear....

  • Global gene expression analysis of Aspergillus nidulans reveals metabolic shift and transcription suppression under hypoxia. Masuo, Shunsuke; Terabayashi, Yasunobu; Shimizu, Motoyuki; Fujii, Tatsuya; Kitazume, Tatsuya; Takaya, Naoki // Molecular Genetics & Genomics;Dec2010, Vol. 284 Issue 6, p415 

    Hypoxia imposes a challenge upon most filamentous fungi that require oxygen for proliferation. Here, we used whole genome DNA microarrays to investigate global transcriptional changes in Aspergillus nidulans gene expression after exposure to hypoxia followed by normoxia. Aeration affected the...

  • Amyloid Beta Resistance in Nerve Cell Lines Is Mediated by the Warburg Effect. Newington, Jordan T.; Pitts, Andrea; Chien, Andrew; Arseneault, Robert; Schubert, David; Cumming, Robert C. // PLoS ONE;2011, Vol. 6 Issue 4, p1 

    Amyloid beta (Aβ) peptide accumulation in the brains of patients with Alzheimer's disease (AD) is closely associated with increased nerve cell death. However, many cells survive and it is important to understand the mechanisms involved in this survival response. Recent studies have shown that...

  • Pyruvate kinase activity in cerebral hemispheres and cerebellum-brainstem of normal and hypoxic-ischemic newborn rats. Yapicioglu, Hacer; Satar, Mehmet; Kayrin, Levent; Tutak, Ercan; Narli, Nejat // Cerebellum;Sep2004, Vol. 3 Issue 3, p152 

    Energy metabolism is affected in hypoxia-ischemia. Changes in the tissue concentrations of the high-energy phosphate reserves occur early during the course of the metabolic insult and with concurrent increases in cellular ADP and AMP leading glycolysis. It has been shown that enzymes of...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics