IL-10 Reduces Levels of Apoptosis in Toxoplasma gondii-Infected Trophoblasts

Zhao, Mingdong; Zhang, Ruijin; Xu, Xiaoyan; Liu, Yang; Zhang, Haixia; Zhai, Xiaoyu; Hu, Xuemei
February 2013
PLoS ONE;Feb2013, Vol. 8 Issue 2, p1
Academic Journal
Background: To analyze the effects of IL-10 on the HLA-G expression and the apoptosis of trophoblasts infected with Toxoplasma gondii. Methods: T. gondii-infected or uninfected human trophoblasts and immortalized human placental BeWo cells were cultured with or without human IL-10. Uninfected and infected cells without IL-10 cells served as controls. HLA-G expression was measured by real-time PCR and flow cytometry, respectively. Cells apoptosis were analyzed by flow cytometry. Apoptosis associated moleculars were measured by real-time PCR and Western bolt. Results: HLA-G expression was increased in the infected trophoblasts and BeWo cells compared to uninfected cells. Treatment of infected cells with IL-10 decreased HLA-G expression compared to infected cells while no change in treatment of uninfected cells compared with uninfected cells. Levels of apoptosis and apoptosis associated caspase-3 and caspase-8 decreased and c-FLIP levels increased in treated infected cells with IL-10 compared to infected cells and no difference in IL-10 treated uninfected cells compared to uninfected cells. Conclusions: IL-10 regulates HLA-G expression in T. gondii-infected trophoblasts. IL-10 treatment of infected trophoblasts reduced levels of apoptosis. This may contribute to the improvement in pregnancy outcomes when women infected with T. gondii treated with IL-10.


Related Articles

  • Proteomic Analysis of Toxoplasma gondii KI-1 Tachyzoites. Si-Hwan Choi; Tae Yun Kim; Sung Goo Park; Guang-Ho Cha; Dae-Whan Shin; Jong-Yil Chai; Young-Ha Lee // Korean Journal of Parasitology;Sep2010, Vol. 48 Issue 3, p195 

    No abstract available.

  • MIC3, a novel cross-protective antigen expressed in Toxoplasma gondii and Neospora caninum. Yang, Daoyu; Liu, Jing; Hao, Pan; Wang, Jing; Lei, Tao; Shan, Dan; Liu, Qun // Parasitology Research;Oct2015, Vol. 114 Issue 10, p3791 

    Microneme protein 3 (MIC3) is an important adhesion molecule expressed by Toxoplasma gondii and Neospora caninum that plays a crucial role in invasion. In our study, we found that recombinant TgMIC3 (rTgMIC3) was recognized by both T. gondii-reactive sera and hyper-immune serum against N....

  • Expression of the tandem enhanced yellow fluorescent marker gene in Toxoplasma gondii. Li, Xuelian; Hao, Yongxin; Chen, Dan; Liu, Qun; Ding, Jun; Zhang, Wei // Parasitology Research;Jul2009, Vol. 105 Issue 1, p287 

    Abstract  Fluorescent protein is a useful tool for monitoring gene expression and studying biological processes of organisms including parasites. To improve the transfection efficiency and fluorescent intensity in Toxoplasma gondii, a new transient expression vector, RGES,...

  • Priming effects of tumor necrosis factor-α on production of reactive oxygen species during Toxoplasma gondii stimulation and receptor gene expression in differentiated HL-60 cells. Kikuchi-Ueda, Takane; Ubagai, Tsuneyuki; Ono, Yasuo // Journal of Infection & Chemotherapy (Springer Science & Business;Dec2013, Vol. 19 Issue 6, p1053 

    Neutrophils are among the principal effector cells that protect against infectious agents, in part by producing reactive oxygen species (ROS) via the actions of tumor necrosis factor-α (TNF-α). In this study, we investigated whether HL-60 cells that had been differentiated into...

  • Real-Time RT-PCR on SAG1 and BAG1 Gene Expression during Stage Conversion in Immunosuppressed Mice Infected with Toxoplasma gondii Tehran Strain. Selseleh, Monavar; Modarressi, Mohammad Hossein; Mohebali, Mehdi; Shojaee, Saeedeh; Eshragian, Mohammad Reza; Selseleh, Mina; Azizi, Ebrahim; Keshavarz, Hossein // Korean Journal of Parasitology;Sep2012, Vol. 50 Issue 3, p199 

    Toxoplasmic encephalitis is caused by reactivation of bradyzoites to rapidly dividing tachyzoites of the apicomplexan parasite Toxoplasma gondii in immunocompromised hosts. Diagnosis of this life-threatening disease is problematic, because it is difficult to discriminate between these 2 stages....

  • Regulation is Diverse Depending on Cell Type and Cell Context. Bartley, J. // Fertility Weekly;9/1/2014, p11 

    The article reports that a study from researchers in Germany focused on differences in regulation of epithelial mesenchymal transition (EMT) different in endometriosis and the endometrium. The study used immunohistochemistry to analyze peritoneal, ovarian, and rectovaginal endometriotic lesions...

  • Simultaneous Detection of Genital Mycoplasma in Women with Genital Infections by PCR. Amirmozafari, N.; Mirnejad, R.; Kazemi, B.; Sariri, E.; Bojari, M. R.; Darkahi, F. Dehdar // Journal of Biological Sciences;2009, Vol. 9 Issue 8, p804 

    The aim of this investigation was to simultaneously detect Mycoplasma hominis, Mycoplasma genitalium and Ureaplasma urealyticum in female patients suffering from genital complications (vaginitis, cervicitis and PID) by PCR. Genital swabs were collected from 210 female patients and subsequently...

  • Changes in T-plastin expression with human trophoblast differentiation. Rao, A. Jagannadha // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Sep2003, Vol. 7 Issue 2, p235 

    During the first trimester of pregnancy, the human placenta is an actively dividing and highly invasive tumour-like tissue, while near term, it represents a fully developed, non-invasive unit. In order to understand the molecular basis of this marked difference in the placental phenotypes, an...

  • Genetic characterization of Toxoplasma gondii in Yunnan black goats (Capra hircus) in southwest China by PCR-RFLP. Qiang Miao; Si-Yang Huang; Si-Yuan Qin; Xin Yu; Yan Yang; Jian-Fa Yang; Xing-Quan Zhu; Feng-Cai Zou // Parasites & Vectors;2015, Vol. 8 Issue 1, p1159 

    Background Toxoplasma gondii is a protozoan parasite that infects almost all warm-blooded animals and human beings. Goats are one of the susceptible animals to T. gondii. However, little is known of genetic diversity of T. gondii in Yunnan black goats in China. The objective of this present...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics