TITLE

[18F]DPA-714: Direct Comparison with [11C]PK11195 in a Model of Cerebral Ischemia in Rats

AUTHOR(S)
Boutin, Hervé; Prenant, Christian; Maroy, Renaud; Galea, James; Greenhalgh, Andrew D.; Smigova, Alison; Cawthorne, Christopher; Julyan, Peter; Wilkinson, Shane M.; Banister, Samuel D.; Brown, Gavin; Herholz, Karl; Kassiou, Michael; Rothwell, Nancy J.
PUB. DATE
February 2013
SOURCE
PLoS ONE;Feb2013, Vol. 8 Issue 2, p1
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Purpose: Neuroinflammation is involved in several brain disorders and can be monitored through expression of the translocator protein 18 kDa (TSPO) on activated microglia. In recent years, several new PET radioligands for TSPO have been evaluated in disease models. [18F]DPA-714 is a TSPO radiotracer with great promise; however results vary between different experimental models of neuroinflammation. To further examine the potential of [18F]DPA-714, it was compared directly to [11C]PK11195 in experimental cerebral ischaemia in rats. Methods: Under anaesthesia, the middle cerebral artery of adult rats was occluded for 60 min using the filament model. Rats were allowed recovery for 5 to 7 days before one hour dynamic PET scans with [11C]PK11195 and/or [18F]DPA-714 under anaesthesia. Results: Uptake of [11C]PK11195 vs [18F]DPA-714 in the ischemic lesion was similar (core/contralateral ratio: 2.84±0.67 vs 2.28±0.34 respectively), but severity of the brain ischemia and hence ligand uptake in the lesion appeared to vary greatly between animals scanned with [11C]PK11195 or with [18F]DPA-714. To solve this issue of inter-individual variability, we performed a direct comparison of [11C]PK11195 and [18F]DPA-714 by scanning the same animals sequentially with both tracers within 24 h. In this direct comparison, the core/contralateral ratio (3.35±1.21 vs 4.66±2.50 for [11C]PK11195 vs [18F]DPA-714 respectively) showed a significantly better signal-to-noise ratio (1.6 (1.3–1.9, 95%CI) fold by linear regression) for [18F]DPA-714. Conclusions: In a clinically relevant model of neuroinflammation, uptake for both radiotracers appeared to be similar at first, but a high variability was observed in our model. Therefore, to truly compare tracers in such models, we performed scans with both tracers in the same animals. By doing so, our result demonstrated that [18F]DPA-714 displayed a higher signal-to-noise ratio than [11C]PK11195. Our results suggest that, with the longer half-life of [18F] which facilitates distribution of the tracer across PET centre, [18F]DPA-714 is a good alternative for TSPO imaging.
ACCESSION #
87624610

 

Related Articles

  • Evaluation of hypoxic tissue dynamics with 18F-FMISO PET in a rat model of permanent cerebral ischemia. Rojas, Santiago; Herance, José Raul; Abad, Sergio; Jiménez, Xavier; Pareto, Deborah; Ruiz, Alba; Torrent, Èlia; Figueiras, Francisca P.; Popota, Foteini; Fernández-Soriano, Francisco J.; Planas, Anna M.; Gispert, Juan D.; Herance, José Raul; Jiménez, Xavier; Torrent, Èlia; Fernández-Soriano, Francisco J; Torrent, Elia // Molecular Imaging & Biology;Jun2011, Vol. 13 Issue 3, p558 

    Purpose: [¹⁸F]Fluoromisonidazole (¹⁸F-FMISO) is a nitroimidazole derivative that has been proposed as a positron emission tomography (PET) radiotracer to detect hypoxic tissue in vivo. This compound accumulates in hypoxic but viable tissue and may be a good candidate...

  • Attenuation of Brain Inflammatory Response after Focal Cerebral Ischemia/Reperfusion with Xuesaitong Injection in Rats. He Wei; Xu Xiao-jun // Chinese Journal of Integrative Medicine;Sep2006, Vol. 12 Issue 3, p203 

    Objective: To investigate the neuro-protective effect of Xuesaitong Injection (XST) on brain inflammatory response after transient focal cerebral ischemia/reperfusion in rats. Methods: Focal cerebral ischemia/reperfusion models of male rats were induced by transient occlusion for 2 h of middle...

  • In vivo and in vitro evaluation of [18 F ]FETO with respect to the adrenocortical and GABAergic system in rats. Mitterhauser, Markus; Wadsak, Wolfgang; Wannegger, Leila; Siegharf, Werner; Viernstein, Helmut; Kletter, Kurt; Dudczak, Robert // European Journal of Nuclear Medicine & Molecular Imaging;2003, Vol. 30 Issue 10, p1398 

    11β-Hydroxylase (CYP11B1, P450[sub11β]) plays an important role in the biosynthesis of cortisol and aldosterone and has been shown to be a good target for the in vivo imaging of adrenocortical incidentalomas in nuclear medicine. [[sup11]C]Metomidate (MTO), a potent inhibitor of this...

  • Increase of 20-HETE synthase after brain ischemia in rats revealed by PET study with 11C-labeled 20-HETE synthase-specific inhibitor. Kawasaki, Toshiyuki; Marumo, Toshiyuki; Shirakami, Keiko; Mori, Tomoko; Doi, Hisashi; Suzuki, Masaaki; Watanabe, Yasuyoshi; Chaki, Shigeyuki; Nakazato, Atsuro; Ago, Yukio; Hashimoto, Hitoshi; Matsuda, Toshio; Baba, Akemichi; Onoe, Hirotaka // Journal of Cerebral Blood Flow & Metabolism;Sep2012, Vol. 32 Issue 9, p1737 

    20-Hydroxyeicosatetraenoic acid (20-HETE), an arachidonic acid metabolite known to be produced after cerebral ischemia, has been implicated in ischemic and reperfusion injury by mediating vasoconstriction. To develop a positron emission tomography (PET) probe for 20-HETE synthase imaging, which...

  • PET imaging of serotoninergic neurotransmission with [11C]DASB and [18F]altanserin after focal cerebral ischemia in rats. Martín, Abraham; Szczupak, Boguslaw; Gómez-Vallejo, Vanessa; Plaza, Sandra; Padró, Daniel; Cano, Ainhoa; Llop, Jordi // Journal of Cerebral Blood Flow & Metabolism;Dec2013, Vol. 33 Issue 12, p1967 

    The use of selective serotonin reuptake inhibitors has shown functional improvement after stroke. Despite this, the role of serotoninergic neurotransmission after cerebral ischemia evolution and its involvement in functional recovery processes are still largely unknown. For this purpose, we...

  • Arundic Acid (ONO-2506) Ameliorates Delayed Ischemic Brain Damage by Preventing Astrocytic Overproduction of S100B. Asano, T.; Mori, T.; Shimoda, T.; Shinagawa, R.; Satoh, S.; Yada, N.; Katsumata, S.; Matsuda, S.; Kagamiishi, Y.; Tateishi, N. // Current Drug Targets - CNS & Neurological Disorders;Apr2005, Vol. 4 Issue 2, p127 

    After focal cerebral ischemia, the infarct volume increases rapidly within acute infarct expansion (initial 12 to 24 h) and continues slowly during delayed infarct expansion (25 to 168 h). While acute infarct expansion represents progressive necrosis within the ischemic core, delayed infarct...

  • The Pathophysiology of Brain Ischemia and Ischemic Preconditioning. Bigdeli, Mohammad Reza; Mohagheghi, Fathemeh // Zahedan Journal of Research in Medical Sciences;Feb2014, Vol. 16 Issue 2, p1 

    Ischemic tolerance can protect the brain against cerebral ischemia and neurodegenerative diseases. However, several studies demonstrate ischemic tolerance by various methods, the exact mechanisms of ischemic tolerance has not been clearly understood. In this study, we first studied brain...

  • SirT1 mediates hyperbaric oxygen preconditioning-induced ischemic tolerance in rat brain. Yan, Wenjun; Fang, Zongping; Yang, Qianzi; Dong, Hailong; Lu, Yan; Lei, Chong; Xiong, Lize // Journal of Cerebral Blood Flow & Metabolism;Mar2013, Vol. 33 Issue 3, p396 

    Our previous studies have shown that hyperbaric oxygen preconditioning (HBO-PC) induces tolerance to cerebral ischemia/reperfusion (I/R). This study aimed to investigate whether SirT1, a class III histone deacetylase, is involved in neuroprotection elicited by HBO-PC in animal and cell culture...

  • The effect of semax and the C-terminal peptide PGP on expression of growth factor genes and receptors in rats under conditions of experimental cerebral ischemia. Dmitrieva, V. G.; Dergunova, L. V.; Povarova, O. V.; Skvortsova, V. I.; Limborskaya, S. A.; Myasoedov, N. F. // Doklady Biochemistry & Biophysics;Sep2008, Vol. 422 Issue 1, p261 

    The article focuses on the effect of semax and c-terminal peptide Pro-Gly-Pro (PGP) on expression of growth factor genes and receptors in rats under conditions of experimental cerebral ischemia. It discusses the composition synthetic peptide semax and c-terminal tripeptide PGP that forms a basis...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics