TITLE

Accuracy of p53 Codon 72 Polymorphism Status Determined by Multiple Laboratory Methods: A Latent Class Model Analysis

AUTHOR(S)
Walter, Stephen D.; Riddell, Corinne A.; Rabachini, Tatiana; Villa, Luisa L.; Franco, Eduardo L.
PUB. DATE
February 2013
SOURCE
PLoS ONE;Feb2013, Vol. 8 Issue 2, p1
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Introduction: Studies on the association of a polymorphism in codon 72 of the p53 tumour suppressor gene (rs1042522) with cervical neoplasia have inconsistent results. While several methods for genotyping p53 exist, they vary in accuracy and are often discrepant. Methods: We used latent class models (LCM) to examine the accuracy of six methods for p53 determination, all conducted by the same laboratory. We also examined the association of p53 with cytological cervical abnormalities, recognising potential test inaccuracy. Results: Pairwise disagreement between laboratory methods occurred approximately 10% of the time. Given the estimated true p53 status of each woman, we found that each laboratory method is most likely to classify a woman to her correct status. Arg/Arg women had the highest risk of squamous intraepithelial lesions (SIL). Test accuracy was independent of cytology. There was no strong evidence for correlations of test errors. Discussion: Empirical analyses ignore possible laboratory errors, and so are inherently biased, but test accuracy estimated by the LCM approach is unbiased when model assumptions are met. LCM analysis avoids ambiguities arising from empirical test discrepancies, obviating the need to regard any of the methods as a “gold” standard measurement. The methods we presented here to analyse the p53 data can be applied in many other situations where multiple tests exist, but where none of them is a gold standard.
ACCESSION #
87624600

 

Related Articles

  • A PROSPECTIVE STUDY OF THE VALUE OF PRE- AND POST-TREATMENT MAGNETIC RESONANCE IMAGING EXAMINATIONS FOR ADVANCED CERVICAL CANCER. CSUTAK, CSABA; ORDEANU, CLAUDIA; NAGY, VIORICA MAGDALENA; POP, DIANA CRISTINA; BOLBOACA, SORANA DANIELA; BADEA, RADU; CHIOREAN, LILIANA; DUDEA, SORIN MARIAN // Clujul Medical;2016, Vol. 89 Issue 3, p410 

    developing countries. It is the only gynecological malignancy that is clinically staged. Staging at the time of diagnosis is crucial for treatment planning. After radiation therapy, clinical examination is limited because of radiation changes. An imaging method relatively unaffected by radiation...

  • P53 tumor suppressor gene product is molecular marker in cervical cancer. Dale, David C.; Federman, Daniel D. // Cortlandt Forum;03/25/98, Vol. 11 Issue 3, p24 

    Reports on the inclusion of p53 tumor suppressor gene product as one of the markers of cervical cancer aside from the human papillomavirus. History of early intercourse with multiple sexual partners; Comparisons between cells with high levels of p53 and cells with low or absent p53.

  • Evaluating the Association between p53 Codon 72 Arg>Pro Polymorphism and Risk of Ovary Cancer: A Meta-Analysis. Alqumber, Mohammed A. A.; Akhter, Naseem; Haque, Shafiul; Panda, Aditya K.; Mandal, Raju K. // PLoS ONE;Apr2014, Vol. 9 Issue 4, p1 

    Aim: Allelic polymorphism in codon 72 of the p53 tumor suppressor gene causes imbalance of p53 protein expression. Earlier studies have shown association between allelic polymorphism in codon 72 of the p53 gene with risk of ovary cancer (OC); however the results are inconclusive and conflicting....

  • Duplex Detection of TP53 Arg72Pro and 16 bp Del/Ins Polymorphisms by a Simple Optimized PCR-RFLP Method. Lajin, B.; Alachkar, A.; Alhaj Sakur, A. // North American Journal of Medical Sciences;May2012, Vol. 4 Issue 5, p212 

    Background: The tumor suppressor gene (TP53) encodes p53, the central protein in the apoptotic pathway which has been shown to be of crucial importance in the development of cancers in addition to a variety of neurodegenerative disorders. Two most commonly studied polymorphisms that were shown...

  • Discrimination of Codon 72 p53 Polymorphisms using High Resolution Melting Curve Method. Abdi, Mohammad; Ghaderi, Bayazid; Amini, Sabrieh; Baban, Arghavan; Najafpour, Sonya; Naderi, Sahar; Pourdad, Shamisa // Asian Journal of Biochemistry;2014, Vol. 9 Issue 3, p142 

    One of the most important polymorphism in p53 gene occurs in codon 73 of exon 4. Individuals who have Arg/Arg allele may have an increase risk for progress of cancer. The present study was aimed to develop a rapid, inexpensive and simple high resolution melting curve analysis method to...

  • A functional BRCA1 coding sequence genetic variant contributes to risk of esophageal squamous cell carcinoma. Zhang, Xiaojiao; Wei, Jinyu; Zhou, Liqing; Zhou, Changchun; Shi, Juan; Yuan, Qipeng; Yang, Ming; Lin, Dongxin // Carcinogenesis;Oct2013, Vol. 34 Issue 10, p2309 

    As a tumor suppressor, breast cancer susceptibility gene 1 (BRCA1) plays a pivotal role in maintaining genomic stability. A functional rs799917 T>C polymorphism located in the BRCA1 coding sequence could influence miR-638-mediated regulation of BRCA1 expression. Therefore, we examined the...

  • Association of p53 codon 72 polymorphism with susceptibility to hepatocellular carcinoma in a Chinese population from northeast Sichuan. JIAJING CAI; YAN CAI; QIANG MA; FAN CHANG; LEI XU; GUOYUAN ZHANG; XIAOLAN GUO // Biomedical Reports;2/1/2017, Vol. 6 Issue 2, p217 

    The p53 tumor suppressor gene is key in tumor development and progression, and the single nucleotide polymorphism (SNP) of the p53 gene codon 72 (p53Arg/Pro) changes the structure of the protein. In addition, it affects its activity, which may affect cancer risk. The aim of the present study was...

  • p53 codon 72 polymorphism is associated with human papillomavirus-related esophageal cancer risk: a meta-analysis. Lianghai Wang; Zhiyu Zhang; Jing Li; Xiaodan Yu; Qianqian Yu; Jun Hou; Feng Li // International Journal of Clinical & Experimental Medicine;2017, Vol. 10 Issue 1, p123 

    Background: p53 codon 72 polymorphism is associated with esophageal cancer (EC). Human papillomavirus (HPV) infection is considered as a risk factor of EC. However, the association of p53 codon 72 polymorphism with the risk of HPV-related EC remains inconsistent. Hence, we aimed to investigate...

  • ATBF1 and NQO1 as candidate targets for allelic loss at chromosome arm 16q in breast cancer: Absence of somatic ATBF1 mutations and no role for the C609T NQO1 polymorphism. Cleton-Jansen, Anne-Marie; Van Eijk, Ronald; Lombaerts, Marcel; Schmidt, Marjanka K.; Van't Veer, Laura J.; Philippo, Katja; Zimmerman, Rhyenne M. E.; Peterse, Johannes L.; Smit, Vincent T. B. H. M.; Van Wezel, Tom; Cornelisse, Cees J. // BMC Cancer;2008, Vol. 8, Special section p1 

    Background: Loss of heterozygosity (LOH) at chromosome arm 16q is frequently observed in human breast cancer, suggesting that one or more target tumor suppressor genes (TSGs) are located there. However, detailed mapping of the smallest region of LOH has not yet resulted in the identification of...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics