Homeostatic Properties and Phenotypic Maturation of Murine CD4+ Pre-Thymic Emigrants in the Thymus

Dong, Jie; Chen, Yu; Xu, Xi; Jin, Rong; Teng, Fei; Yan, Fan; Tang, Hui; Li, Pingping; Sun, Xiuyuan; Li, Yan; Wu, Hounan; Zhang, Yu; Ge, Qing
February 2013
PLoS ONE;Feb2013, Vol. 8 Issue 2, p1
Academic Journal
After a tightly regulated developmental program in the thymus, “mature” single positive (SP) thymocytes leave the thymus and enter the periphery. These newly arrived recent thymic emigrants (RTEs) are phenotypically and functionally immature, and will complete a dynamic maturation in the peripheral lymphoid organs before being licensed to be resident naïve T cells. To study the early events occurring in the RTE maturation process, we identified the phenotype of CD4+ pre-RTEs, a population of CD4+ SP thymocytes that have acquired the thymus egress capability. Compared to peripheral naïve T cells, CD4+ pre-RTEs displayed superior survival capability in lymphoreplete mice and faster proliferation under lymphopenic condition. The differences in Bcl2/Bim expression and/or heightened IL-7 signaling pathway may account for the pre-RTEs’ better responsiveness to homeostatic signals. Qa2, the expression of which indicates the phenotypic maturation of SPs and RTEs, was found to be upregulated in CD4+ pre-RTEs in thymic perivascular space. Migratory dendritic cells that surround this region contribute to Qa2 expression in pre-RTEs. The dendritic cell-driven Qa2 induction of CD4+ pre-RTEs is independent of MHC class II and Aire molecules.


Related Articles

  • The ocular surface : from physiology to the ocular allergic diseases. Galicia-Carreón, Jorge; Santacruz, Concepción; Hong, Enrique; Jiménez-Martínez, María C. // Revista Alergia de Mexico;oct-dic2013, Vol. 60 Issue 4, p172 

    Allergic conjunctivitis (AC) is an inflammation of the con-junctiva secondary to an immune response to exogenous antigens, usually called allergens. In fact, AC is a syndrome that involves the entire ocular surface, including conjunctiva, lids, cornea, and tear film. The signs and symptoms of AC...

  • Red Blood Cells as Modulators of T Cell Growth and Survival. Pereira, Carlos F.; Fonseca, Ana M.; Arosa, Fernando A. // Current Pharmaceutical Design;Jan2004, Vol. 10 Issue 2, p191 

    T cell homeostasis is largely controlled by a balance between cell death and survival and anomalies in either process account for a number of diseases linked to excessive or faulty T cell growth. Yet, the influence that cells outside the immunological system have on these processes has only...

  • Cytokines and T-Cell Homeostasis in HIV Infection. Freeman, Michael L.; Shive, Carey L.; Nguyen, Thao P.; Younes, Souheil-Antoine; Panigrahi, Soumya; Lederman, Michael M. // Journal of Infectious Diseases;2016 Supplement, Vol. 214, pS51 

    Untreated human immunodeficiency virus (HIV) infection is characterized by progressive CD4(+) T-cell depletion and CD8(+) T-cell expansion, and CD4(+) T-cell depletion is linked directly to the risk for opportunistic infections and infection-associated mortality. With suppression of HIV...

  • Dual Role of miR-21 in CD4+ T-Cells: Activation-Induced miR-21 Supports Survival of Memory T-Cells and Regulates CCR7 Expression in Naive T-Cells. Smigielska-Czepiel, Katarzyna; van den Berg, Anke; Jellema, Pytrick; Slezak-Prochazka, Izabella; Maat, Henny; van den Bos, Hilda; van der Lei, Roelof Jan; Kluiver, Joost; Brouwer, Elisabeth; Boots, Anne Mieke H.; Kroesen, Bart-Jan // PLoS ONE;Oct2013, Vol. 8 Issue 10, p1 

    Immune cell-type specific miRNA expression patterns have been described but the detailed role of single miRNAs in the function of T-cells remains largely unknown. We investigated the role of miR-21 in the function of primary human CD4+ T-cells. MiR-21 is substantially expressed in T-cells with a...

  • The Foxp3 interactome: a network perspective of Treg cells. Hori, Shohei // Nature Immunology;Oct2012, Vol. 13 Issue 10, p943 

    No abstract available.

  • Human thymic stromal lymphopoietin promotes dendritic cell-mediated CD4+ T cell homeostatic expansion. Watanabe, Norihiko; Hanabuchi, Shino; Soumelis, Vassili; Wei Yuan; Ho, Stephen; Malefyt, Rene de Waal; Yong-Jun Liu // Nature Immunology;Apr2004, Vol. 5 Issue 4, p426 

    T cell homeostasis is a self-regulating process for maintaining the size of the peripheral T cell pool. Although dendritic cells (DCs) seem to be important in T cell homeostasis, the molecular regulation of DC-mediated T cell homeostasis is unknown. We show that human DCs activated by thymic...

  • Impact of the TCR Signal on Regulatory T Cell Homeostasis, Function, and Trafficking. Joong Kyu Kim; Klinger, Mark; Benjamin, Jonathan; Yuanyuan Xiao; Erle, David J.; Littman, Dan R.; Killeen, Nigel // PLoS ONE;2009, Vol. 4 Issue 8, p1 

    Signaling through the T cell antigen receptor (TCR) is important for the homeostasis of naïve and memory CD4+ T cells. The significance of TCR signaling in regulatory T (Treg) cells has not been systematically addressed. Using an Ox40-cre allele that is prominently expressed in Treg cells,...

  • HIV-1 receptors and cell tropism. Clapham, Paul R.; McKnight, Áine // British Medical Bulletin;Sep2001, Vol. 58 Issue 1, p43 

    HIV virus particles interact with several receptors on cell surfaces. Two receptors, CD4 and a co-receptor act sequentially to trigger fusion of viral and cellular membranes and confer virus entry into cells. For HIV-1, the chemokine receptor CCR5 is the predominant co-receptor exploited for...

  • Indirubin Increases CD4+CD25+Foxp3+ Regulatory T Cells to Prevent Immune Thrombocytopenia in Mice. Zhang, Aijun; Ning, Bin; Sun, Nianzheng; Wei, Jianlu; Ju, Xiuli // PLoS ONE;11/16/2015, Vol. 10 Issue 11, p1 

    Indirubin, a traditional Chinese medicine, is used to treat autoimmune diseases in clinics. However, the effects of indirubin on the immunosuppressive CD4+CD25+Foxp3+ regulatory T cells (Treg) have not been addressed. Thus, we aimed to investigate the effects of indirubin on CD4+CD25+Treg cells...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics