Non-Linear Optical Microscopy Sheds Light on Cardiovascular Disease

Caorsi, Valentina; Toepfer, Christopher; Sikkel, Markus B.; Lyon, Alexander R.; MacLeod, Ken; Ferenczi, Mike A.
February 2013
PLoS ONE;Feb2013, Vol. 8 Issue 2, p1
Academic Journal
Many cardiac diseases have been associated with increased fibrosis and changes in the organization of fibrillar collagen. The degree of fibrosis is routinely analyzed with invasive histological and immunohistochemical methods, giving a limited and qualitative understanding of the tissue's morphological adaptation to disease. Our aim is to quantitatively evaluate the increase in fibrosis by three-dimensional imaging of the collagen network in the myocardium using the non-linear optical microscopy techniques Two-Photon Excitation microscopy (TPE) and Second Harmonic signal Generation (SHG). No sample staining is needed because numerous endogenous fluorophores are excited by a two-photon mechanism and highly non-centrosymmetric structures such as collagen generate strong second harmonic signals. We propose for the first time a 3D quantitative analysis to carefully evaluate the increased fibrosis in tissue from a rat model of heart failure post myocardial infarction. We show how to measure changes in fibrosis from the backward SHG (BSHG) alone, as only backward-propagating SHG is accessible for true in vivo applications. A 5-fold increase in collagen I fibrosis is detected in the remote surviving myocardium measured 20 weeks after infarction. The spatial distribution is also shown to change markedly, providing insight into the morphology of disease progression.


Related Articles

  • Which is your diagnosis? Nacif, Marcelo Souto; Noureldin, Radwa A. A.; Sibley, Christopher T.; Turkbey, Evrim B.; Lima, João A. C.; Bluemke, David A. // Radiologia Brasileira;sep/oct2010, Vol. 43 Issue 5, preceding p281 

    The article focuses on the evaluation of a female diagnosed with heart failure (HF) with strain analysis and tissue characterization for fibrosis through cardiac magnetic resonance imaging (MRI). It notes the evolution of cardiovascular magnetic resonance (CMR) as a technology that is used for...


    INTRODUCTION: Many recent clinical observation have revealed the presence of both pulmonary and tracheobronchial lymphadenopathy in patients with congestive heart failure. That lymphadenopathy is reversible and disappears after treatment for heart failure. In this paper we study the venous...

  • ARB reduces hospitalizations, CV mortality in heart failure.  // Formulary;Oct2003, Vol. 38 Issue 10, p576 

    Presents results of the Candesartan in Heart Failure: Assessment of Reduction in Mortality and Morbidity (CHARM), a clinical program presented at the 2003 annual meeting of the European Society of Cardiology in Vienna, Austria. Finding that Candesartan reduces the incidence of cardiovascular...

  • Intracellular or extracellular heat shock protein 70 differentially regulates cardiac remodelling in pressure overload mice. Wen-Feng Cai; Xiao-Wei Zhang; Hui-Min Yan; Yong-Gang Ma; Xiao-Xing Wang; Jun Yan; Bing-Mu Xin; Xiao-Xi Lv; Qing-Qing Wang; Zi-Yan Wang; Hong-Zhen Yang; Zhuo-Wei Hu // Cardiovascular Research;Oct2010, Vol. 88 Issue 1, p140 

    Aims: Innate and adaptive immune responses are associated with the development of hypertension-induced myocardial hypertrophy and fibrosis. As a result, we investigated whether heat shock protein (HSP) 70, which is a molecule of damage-associated molecular patterns, could induce inflammation in...

  • Cardiac and vascular metal deposition with high mortality in nephrogenic systemic fibrosis. Swaminathan, S.; High, W. A.; Ranville, J.; Horn, T. D.; Hiatt, K.; Thomas, M.; Brown, H. H.; Shah, S. V. // Kidney International;Jun2008, Vol. 73 Issue 12, p1413 

    Nephrogenic systemic fibrosis is a severe disabling disease that can follow gadolinium-based contrast exposure. In this study we analyzed the clinical and laboratory records of patients with nephrogenic systemic fibrosis who had a history of exposure to gadolinium-based contrast media and...

  • Acute Idiopathic Heart Failure Following Laparoscopic Myotomy for Achalasia of the Esophagus. Ural, Kelly G.; Landreneau, Rodney J.; Menard, Grayson W.; Gomez, Daniel // Ochsner Journal;Spring2015, Vol. 15 Issue 1, p83 

    Background: Stress-induced cardiomyopathy, also known as takotsubo cardiomyopathy, is not fully understood. It is thought to occur in patients who have signs and symptoms consistent with acute myocardial infarction but display no obstructive coronary lesions during heart catheterization....

  • Assessment of the early stage of cardiac remodeling of spontaneously hypertensive heart failure rats using the quantitative 3-dimensional analysis provided by acipimox-enhanced FDG-PET. Maskali, Fatiha; Poussier, Sylvain; Louis, Huguette; Boutley, Henri; Lhuillier, Mickael; Thornton, Simon; Karcher, Gilles; Lacolley, Patrick; Marie, Pierre // International Journal of Cardiovascular Imaging;Feb2014, Vol. 30 Issue 2, p449 

    Spontaneously hypertensive heart failure rats (SHHF) appear to constitute an original model for analyzing the evolution of the metabolic syndrome towards heart failure. This study aimed to characterize early cardiac dysfunction and remodeling in SHHF rats: (1) as compared with spontaneously...

  • Parental cardiometabolic factors, not obesity, help predict offspring risks.  // Endocrine Today;Jun2014, Vol. 12 Issue 6, p16 

    The article presents a study concerning the use of parental cardiometabolic factors measurement as an effective approach to determine the risks of cardiovascular diseases in children.

  • Nanoscale Examination of Microdamage in Sheep Cortical Bone Using Synchrotron Radiation Transmission X-Ray Microscopy. Brock, Garry R.; Kim, Grace; Ingraffea, Anthony R.; Andrews, Joy C.; Pianetta, Piero; van der Meulen, Marjolein C. H. // PLoS ONE;Mar2013, Vol. 8 Issue 3, p1 

    Microdamage occurs in bone through repeated and excessive loading. Accumulation of microdamage weakens bone, leading to a loss of strength, stiffness and energy dissipation in the tissue. Imaging techniques used to examine microdamage have typically been limited to the microscale. In the current...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics