Identification of Injury Specific Proteins in a Cell Culture Model of Traumatic Brain Injury

Lööv, Camilla; Shevchenko, Ganna; Geeyarpuram Nadadhur, Aishwarya; Clausen, Fredrik; Hillered, Lars; Wetterhall, Magnus; Erlandsson, Anna
February 2013
PLoS ONE;Feb2013, Vol. 8 Issue 2, p1
Academic Journal
The complicated secondary molecular and cellular mechanisms following traumatic brain injury (TBI) are still not fully understood. In the present study, we have used mass spectrometry to identify injury specific proteins in an in vitro model of TBI. A standardized injury was induced by scalpel cuts through a mixed cell culture of astrocytes, oligodendrocytes and neurons. Twenty-four hours after the injury, cell culture medium and whole-cell fractions were collected for analysis. We found 53 medium proteins and 46 cell fraction proteins that were specifically expressed after injury and the known function of these proteins was elucidated by an extensive literature survey. By using time-lapse microscopy and immunostainings we could link a large proportion of the proteins to specific cellular processes that occur in response to trauma; including cell death, proliferation, lamellipodia formation, axonal regeneration, actin remodeling, migration and inflammation. A high percentage of the proteins uniquely expressed in the medium after injury were actin-related proteins, which normally are situated intracellularly. We show that two of these, ezrin and moesin, are expressed by astrocytes both in the cell culture model and in mouse brain subjected to experimental TBI. Interestingly, we found many inflammation-related proteins, despite the fact that cells were present in the culture. This study contributes with important knowledge about the cellular responses after trauma and identifies several potential cell-specific biomarkers.


Related Articles

  • Endogenous amyloidogenesis in long-term rat hippocampal cell cultures. Bertrand, Sarah J.; Aksenova, Marina V.; Aksenov, Micheal Y.; Mactutus, Charles F.; Booze, Rosemarie M. // BMC Neuroscience;2011, Vol. 12 Issue 1, p38 

    Background: Long-term primary neuronal cultures are a useful tool for the investigation of biochemical processes associated with neuronal senescence. Improvements in available technology make it possible to observe maturation of neural cells isolated from different regions of the rodent brain...

  • Intrinsic indicators for specimen degradation. Li, Jie; Kil, Catherine; Considine, Kelly; Smarkucki, Bartosz; Stankewich, Michael C; Balgley, Brian; Vortmeyer, Alexander O // Laboratory Investigation (00236837);Feb2013, Vol. 93 Issue 2, p242 

    Variable degrees of molecular degradation occur in human surgical specimens before clinical examination and severely affect analytical results. We therefore initiated an investigation to identify protein markers for tissue degradation assessment. We exposed 4 cell lines and 64 surgical/autopsy...

  • Proteome analyses of cultured astrocytes treated with MK-801 and clozapine: similarities with schizophrenia. Martins-de-Souza, Daniel; Lebar, Maria; Turck, Christoph // European Archives of Psychiatry & Clinical Neuroscience;Apr2011, Vol. 261 Issue 3, p217 

    On the basis of impaired glutamatergic transmission and the potential role of astrocytes in schizophrenia, we treated cultured astrocytes with MK-801, an NMDA-receptor antagonist, to investigate whether the resulting proteome changes are similar to those we found in our earlier proteome analysis...

  • Low-power 808-nm laser irradiation inhibits cell proliferation of a human-derived glioblastoma cell line in vitro. Murayama, Hideyuki; Sadakane, Kei; Yamanoha, Banri; Kogure, Shinichi // Lasers in Medical Science;Jan2012, Vol. 27 Issue 1, p87 

    It has been reported that low-power laser irradiation (LLI) can modulate various biological processes including cell proliferation. Some reports suggest that LLI interferes with the cell cycle and inhibits cell proliferation, while others suggest that LLI has a stimulatory effect. Mechanisms...

  • Effects of Chemotherapeutics on Organotypic Corticostriatal Slice Cultures Identified by A Panel of Fluorescent and Immunohistochemical Markers. Nørregaard, Annette; Jensen, Stine; Kolenda, Jesper; Aaberg-Jessen, Charlotte; Christensen, Karina; Jensen, Poul; Schrøder, Henrik; Kristensen, Bjarne // Neurotoxicity Research;Jul2012, Vol. 22 Issue 1, p43 

    Effects of chemotherapeutics on glioma cell lines and spheroids are usually investigated without evaluating the effects of chemotherapeutics on normal brain tissue. To perform such investigations, the aim of this study was to establish a panel of markers for detection of general cell death and...

  • Direct Injection of Redissolved Cell Culture Media into a Single-Column Liquid Chromatography Coupled to Mass Spectrometry for the Measurement of PGE2. Araujo, Pedro; Zhen-Yu Du; Thu-Thao Nguyen; Holen, Elisabeth // Open Analytical Chemistry Journal;2008, Vol. 2, p62 

    The traditional approach to assessing eicosanoids in cell cultures by liquid or gas chromatography coupled to mass spectrometry involves time-consuming procedures such as derivatisation, degradation, solid-phase extraction, liquidliquid extraction, thin layer chromatography and sometimes,...

  • Co-culture of neuroepithelial stem cells with interstitial cells of Cajal results in neuron differentiation. Bin Zhao; Wei Liu; Rongde Wu // International Journal of Clinical & Experimental Medicine;2015, Vol. 8 Issue 7, p10437 

    Objective: The interstitial cells of Cajal (ICCs) interact morphologically and functionally with the elements of the enteric nervous system in the digestive tract. However, direct evidence that ICCs participate in the differentiation of the enteric nervous system is lacking. In this work, we...

  • Qualitative serum organic acid profiles of HIV-infected individuals not on antiretroviral treatment. Williams, Aurelia; Koekemoer, Gerhard; Lindeque, Zander; Reinecke, Carolus; Meyer, Debra // Metabolomics;Oct2012, Vol. 8 Issue 5, p804 

    The first application of gas chromatography mass spectrometry (GC-MS) metabolomics to the analysis of organic acid profiles in sera of asymptomatic human immunodeficiency virus (HIV)-infected individuals ( n = 18) compared to uninfected controls ( n = 21), is reported here. Several organic acids...

  • Quantitative proteomics: the next logical step in molecular phenotyping. Veenstra, Timothy D.; Blonder, Josip // Briefings in Functional Genomics & Proteomics;Mar2009, Vol. 8 Issue 2, p89 

    The authors reflect on the increased potential to identify a large quantity of proteins from a sample of proteome through mass spectrometry (MS). They state that the sensitivity and specificity of MS make it the preferred tool for quantitating metabolites. They cite the conventional ideas on the...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics