Eomesodermin of Atlantic Salmon: An Important Regulator of Cytolytic Gene and Interferon Gamma Expression in Spleen Lymphocytes

Kumari, Jaya; Bøgwald, Jarl; Dalmo, Roy A.
February 2013
PLoS ONE;Feb2013, Vol. 8 Issue 2, p1
Academic Journal
Eomesodermin (Eomes), a T-bet homologue expressed in activated CD8+T cells was recently proposed to act as a master regulator of cytotoxic CD8+ T cell effector function and offers an exciting avenue for future exploration. Here, we have identified and characterized the full-length Atlantic salmon Eomes cDNA (2477 bp). Promoter analysis of the salmon Eomes showed the presence of important putative transcription binding sites like SP1, FOXO, Oct-1, SMAD, STAT, IRF, and Ets-1. The basal core region responsible for the promoter activity was located between base −199 and +59. Quantitative PCR analysis revealed that the Atlantic salmon Eomes was ubiquitously expressed in all the tissues studied but strongly expressed in the ovary, spleen, brain, and the head kidney. Moreover, the involvement of Eomes in Atlantic salmon immune response and its relation with the cytolytic activity was demonstrated by investigating the early time dependent expression profile of Eomes and CD8α followed by high interferon gamma (IFN-γ) and granzyme A expression during challenge with live Aeromonas salmonicida and Infectious Pancreatic Necrosis (IPN) virus. Therefore, we further analyzed the regulated expression and function of this transcription factor in spleen lymphocytes. Overexpression of Eomes induced IFN-γ, and granzyme A expression but not perforin expression, whereas small interfering RNA (siRNA) mediated suppression of Eomes expression led to significantly reduced IFN-γ production. Thus, Eomes may be critical in cytolytic gene expression and function in fish similar to mammals. Furthermore, IFN-α, and mitogens induced Eomes expression. Taken together, this is the first study on the promoter activity and regulatory role of Eomes in fish.


Related Articles

  • Cloning, chromosomal location, and tissue expression of the gene for pig interleukin-18. Fournout, S.; Dozois, C. M.; Yerle, M.; Pinton, P.; Fairbrother, J.M.; Oswald, E.; Oswald, I. P. // Immunogenetics;Apr2000, Vol. 51 Issue 4/5, p358 

    Interleukin 18 (IL-18), a recently described cytokine, plays an important role in the cell-mediated immune response, in particular through its ability to induce the production of interferon (IFN)-γ. We cloned pig IL-18 cDNA from the intestinal epithelial cell line IPI-2I using a reverse...

  • Identification and characterization of interferon regulatory factor-1 from orange-spotted grouper (Epinephelus coioides). Yan Shi; Jing-Kui Yin; Qi-Ya Zhang; Jian-Fang Gui // Molecular Biology Reports;Mar2010, Vol. 37 Issue 3, p1483 

    Abstract  Interferon-regulatory factor 1 (IRF-1) is the first member of IRF family, which is involved in many biological processes such as immune response, antiviral defense, cell growth regulation, and apoptosis. In this study, an IRF-1 gene, EcIRF-1, was isolated and characterized from...

  • Type 1 Interferons and Antiviral CD8 T-Cell Responses. Welsh, Raymond M.; Bahl, Kapil; Marshall, Heather D.; Urban, Stina L. // PLoS Pathogens;Jan2012, Vol. 8 Issue 1, Special section p1 

    The authors offer clinical information on the effect of type 1 interferons (IFN) on CD8 T-cell responses. They explain the impact of type 1 IFN on T cells and natural killer (NK) cells during immune responses. They describe the mechanisms of IFN signaling and gene activation, along with the...

  • Expression analysis of KAP9.2 and Hoxc13 genes during different cashmere growth stages by qRT-PCR method. Wang, X.; Xu, H.; Li, T.; Qu, L.; Zhao, Z.; Zhang, Z. // Molecular Biology Reports;Sep2014, Vol. 41 Issue 9, p5665 

    Keratin-associated protein 9.2 ( KAP9.2) and Homeobox C13 ( Hoxc13) genes were chosen to study because of their biological functions involving hair formation. KAP9.2 gene belongs to the ultra high sulfur KAPs, which is important for hair formation and may have association with cashmere. Hoxc13...

  • Census of cytosolic aminopeptidase activity reveals two novel cytosolic aminopeptidases. Akkad, Nadja; Schatz, Mark; Dengjel, Jörn; Tenzer, Stefan; Schild, Hansjörg // Medical Microbiology & Immunology;Nov2012, Vol. 201 Issue 4, p463 

    Activation of CD8 cytotoxic T cells is crucial for the adaptive immune response against viral infections and the control of malignant transformed cells. Together with activation of costimulatory molecules like CD3 and CD28, CD8 T cells need activation of their unique T cell receptor via...

  • Self-reactive T cells: suppressing the suppressors. Becker, Jürgen; Straten, Per; Andersen, Mads // Cancer Immunology, Immunotherapy;Apr2014, Vol. 63 Issue 4, p313 

    The immune system is a tightly regulated and complex system. An important part of this immune regulation is the assurance of tolerance toward self-antigens to maintain immune homeostasis. However, in recent years, antigen-specific cellular immune responses toward several normal self-proteins...

  • Hypoxia-inducible factors enhance the effector responses of CD8+ T cells to persistent antigen. Doedens, Andrew L; Phan, Anthony T; Stradner, Martin H; Fujimoto, Jessica K; Nguyen, Jessica V; Yang, Edward; Johnson, Randall S; Goldrath, Ananda W // Nature Immunology;Nov2013, Vol. 14 Issue 11, p1173 

    Cytolytic activity by CD8+ cytotoxic T lymphocytes (CTLs) is a powerful strategy for the elimination of intracellular pathogens and tumor cells. The destructive capacity of CTLs is progressively dampened during chronic infection, yet the environmental cues and molecular pathways that influence...

  • The transcription factor IRF4 is essential for TCR affinity-mediated metabolic programming and clonal expansion of T cells. Man, Kevin; Miasari, Maria; Shi, Wei; Xin, Annie; Henstridge, Darren C; Preston, Simon; Pellegrini, Marc; Belz, Gabrielle T; Smyth, Gordon K; Febbraio, Mark A; Nutt, Stephen L; Kallies, Axel // Nature Immunology;Nov2013, Vol. 14 Issue 11, p1155 

    During immune responses, T cells are subject to clonal competition, which leads to the predominant expansion of high-affinity clones; however, there is little understanding of how this process is controlled. We found here that the transcription factor IRF4 was induced in a manner dependent on...

  • ADAM17-Mediated Processing of TNF-α Expressed by Antiviral Effector CD8+ T Cells Is Required for Severe T-Cell-Mediated Lung Injury. DeBerge, Matthew P.; Ely, Kenneth H.; Cheng, Guang-Shing; Enelow, Richard I. // PLoS ONE;Nov2013, Vol. 8 Issue 11, p1 

    Influenza infection in humans evokes a potent CD8+ T-cell response, which is important for clearance of the virus but may also exacerbate pulmonary pathology. We have previously shown in mice that CD8+ T-cell expression of TNF-α is required for severe and lethal lung injury following...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics