TITLE

Apocynin Prevents Vascular Effects Caused by Chronic Exposure to Low Concentrations of Mercury

AUTHOR(S)
Rizzetti, Danize A.; Torres, João Guilherme D.; Escobar, Alyne G.; Peçanha, Franck M.; Santos, Francielli W.; Puntel, Robson L.; Alonso, María J.; Briones, Ana M.; Salaices, Mercedes; Vassallo, Dalton V.; Wiggers, Giulia A.
PUB. DATE
February 2013
SOURCE
PLoS ONE;Feb2013, Vol. 8 Issue 2, p1
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Mercury increases the risk of cardiovascular disease and oxidative stress and alters vascular reactivity. This metal elicits endothelial dysfunction causing decreased NO bioavailability via increased oxidative stress and contractile prostanoid production. NADPH oxidase is the major source of reactive oxygen species (ROS) in the vasculature. Our aim was to investigate whether treatment with apocynin, an NADPH oxidase inhibitor, prevents the vascular effects caused by chronic intoxication with low concentrations of mercury. Three-month-old male Wistar rats were treated for 30 days with a) intramuscular injections (i.m.) of saline; b) HgCl2 (i.m. 1st dose: 4.6 µg/kg, subsequent doses: 0.07 µg/kg/day); c) Apocynin (1.5 mM in drinking water plus saline i.m.); and d) Apocynin plus HgCl2. The mercury treatment resulted in 1) an increased aortic vasoconstrictor response to phenylephrine and reduced endothelium-dependent responses to acetylcholine; 2) the increased involvement of ROS and vasoconstrictor prostanoids in response to phenylephrine, whereas the endothelial NO modulation of such responses was reduced; and 3) the reduced activity of aortic superoxide dismutase (SOD) and glutathione peroxidase (GPx) and increased plasma malondialdehyde (MDA) levels. Treatment with apocynin partially prevented the increased phenylephrine responses and reduced the endothelial dysfunction elicited by mercury treatment. In addition, apocynin treatment increased the NO modulation of vasoconstrictor responses and aortic SOD activity and reduced plasma MDA levels without affecting the increased participation of vasoconstrictor prostanoids observed in aortic segments from mercury-treated rats. Conclusions: Mercury increases the vasoconstrictor response to phenylephrine by reducing NO bioavailability and increasing the involvement of ROS and constrictor prostanoids. Apocynin protects the vessel from the deleterious effects caused by NADPH oxidase, but not from those caused by prostanoids, thus demonstrating a two-way action.
ACCESSION #
87624016

 

Related Articles

  • New roles for old pathways? A circuitous relationship between reactive oxygen species and cyclo-oxygenase in hypertension. HERNANZ, Raquel; BRIONES, Ana M.; SALAICES, Mercedes; ALONSO, María J. // Clinical Science;Jan2014, Vol. 126 Issue 2, p111 

    Elevated production of prostanoids from the constitutive (COX-1) or inducible (COX-2) cyclo-oxygenases has been involved in the alterations in vascular function, structure and mechanical properties observed in cardiovascular diseases, including hypertension. In addition, it is well known that...

  • NADPH oxidases: key modulators in aging and age-related cardiovascular diseases? Sahoo, Sanghamitra; Meijles, Daniel N.; Pagano, Patrick J. // Clinical Science;Mar2016, Vol. 130 Issue 5, p317 

    Reactive oxygen species (ROS) and oxidative stress have long been linked to aging and diseases prominent in the elderly such as hypertension, atherosclerosis, diabetes and atrial fibrillation (AF). NADPH oxidases (Nox) are a major source of ROS in the vasculature and are key players in mediating...

  • The effect of melatonin on periodontal bone destruction and oxidative stres parameters in experimental periodontitis. Özdem, Muhsin; Kırzıoğlu, F. Yeşim // SDU Journal of Health Science Institute / SDÃœ Saglik Bilimleri ;2012, Vol. 3 Issue 1, p58 

    The purpose of this study was to evaluate the effect of melatonin on periodontal bone destruction and serum malondialdehyde (MDA), superoxidedismutase (SOD), glutathione peroxidase (GSH-Px) and determine the level of gingival melatonin in experimental rat periodontitis. his study, 30 Wistar...

  • Influence of endothelial dysfunction on telomere length in subjects with metabolic syndrome: LIPGENE study. González-Guardia, Lorena; Yubero-Serrano, Elena; Rangel-Zuñiga, Oriol; Marin, Carmen; Camargo, Antonio; Pérez-Martínez, Pablo; Delgado-Lista, Javier; Gómez-Delgado, Francisco; Garcia-Rios, Antonio; Tinahones, Francisco; Roche, Helen; Pérez-Jiménez, Francisco; López-Miranda, José // Age;Aug2014, Vol. 36 Issue 4, p1 

    Previous evidences support that increased oxidative stress (OxS) may play an important role in metabolic syndrome (MetS) and both are closely linked to vascular dysfunction. This study determined whether there is a relationship between endothelial function and relative telomere length (RTL) in...

  • Glycemic Variability and Oxidative Stress: A Link between Diabetes and Cardiovascular Disease? Yoshifumi Saisho // International Journal of Molecular Sciences;Oct2014, Vol. 15 Issue 10, p18381 

    Diabetes is associated with a two to three-fold increase in risk of cardiovascular disease. However, intensive glucose-lowering therapy aiming at reducing HbA1c to a near-normal level failed to suppress cardiovascular events in recent randomized controlled trials. HbA1c reflects average glucose...

  • The Relationship Between Blood Antioxidant Enzyme Levels and Genotype During Migraine Attack and Initial Periods in Migraine Patients. Özbey, Ülkü; Özel, Seda; Seyran, Ayşe; Erişir, Mine; Berilgen, M. Said // Turkish Journal of Biochemistry / Turk Biyokimya Dergisi;Dec2011, Vol. 36 Issue 4, p310 

    Objective: In recent years, researchers have been focused on the importance of oxidative stress on migraine pathogenesis. We aimed to investigate the roles of Superoxide Dismutase (SOD), Glutathione Peroxidase (GSH-Px), Catalase (CAT), antioxidant Glutathione (GSH) and Malondialdehyde (MDA) of a...

  • Oxidative stress parameters and their correlation with clinical, metabolic and polysomnographic parameters in severe obstructive sleep apnea syndrome. Asker, Selvi; Asker, Muntecep; Sarikaya, Eren; Sunnetcioglu, Aysel; Aslan, Mehmet; Demir, Halit // International Journal of Clinical & Experimental Medicine;2015, Vol. 8 Issue 7, p11449 

    The aim of the present study was to assess the levels of oxidative stress markers, catalase (CAT), glutathione peroxidase (GPX) and malondialdehyde (MDA) in severe OSAS and to investigate any correlation between oxidative stress markers and clinical, metabolic and polysomnographic parameters. A...

  • Serum oxidant and antioxidant status of patients with chronic tension-type headache: possible effects of medical treatment. Gökçe Çokal, Burcu; Aytaç, Bilal; Durak, Zahide; Güneş, Hafize; Öztürk, Bahadır; Keskin Güler, Selda; Durak, İlker; Yoldaş, Tahir; Gökçe Çokal, Burcu; Aytaç, Bilal; Durak, Zahide Esra; Güneş, Hafize Nalan; Öztürk, Bahadır; Keskin Güler, Selda; Durak, İlker; Yoldaş, Tahir Kurtuluş // Neurological Sciences;Oct2015, Vol. 36 Issue 10, p1771 

    Tension-type headache (TTH) is one of the most common and costly primary types of headache in clinical practice, with an unknown etiology. This study assessed to investigate oxidative and antioxidative status in patients with chronic tension-type headache (CTTH), and to evaluate possible effect...

  • Long-term improvement of oxidative stress via kidney transplantation ameliorates serum sulfatide levels. Kamijo, Yuji; Wang, Lixuan; Matsumoto, Akihiro; Nakajima, Takero; Hashimoto, Koji; Higuchi, Makoto; Kyogashima, Mamoru; Aoyama, Toshifumi; Hara, Atsushi // Clinical & Experimental Nephrology;Dec2012, Vol. 16 Issue 6, p959 

    Background: Oxidative stress (OS) is a strong risk factor for cardiovascular disease (CVD). The incidence of CVD is lower among kidney transplantation (KT) recipients than hemodialysis patients, and the reduction in OS may be one reason for this difference. Recently, serum sulfatides were...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics