TITLE

eIF4GI Facilitates the MicroRNA-Mediated Gene Silencing

AUTHOR(S)
Ryu, Incheol; Park, Ji Hoon; An, Sihyeon; Kwon, Oh Sung; Jang, Sung Key
PUB. DATE
February 2013
SOURCE
PLoS ONE;Feb2013, Vol. 8 Issue 2, p1
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
MicroRNAs (miRNAs) are small noncoding RNAs that mediate post-transcriptional gene silencing by binding to complementary target mRNAs and recruiting the miRNA-containing ribonucleoprotein complexes to the mRNAs. However, the molecular basis of this silencing is unclear. Here, we show that human Ago2 associates with the cap-binding protein complex and this association is mediated by human eIF4GI, a scaffold protein required for the translation initiation. Using a cap photo-crosslinking method, we show that Ago2 closely associates with the cap structure. Taken together, these data suggest that eIF4GI participates in the miRNA-mediated post-transcriptional gene silencing by promoting the association of Ago2 with the cap-binding complex.
ACCESSION #
87623950

 

Related Articles

  • RNA WORLD. VLASSOV, V. V.; VOROB'EV, P. E. // SCIENCE First Hand;2015, Vol. 40 Issue 1, p44 

    has been long considered that ribonucleic acid (RNA), the closest "relative" of the famous DNA, performs only utility functions in the body, being a mere messenger in most intricate cellular processes. Although it was recognized that RNA could play the leading role at early stages in the...

  • Hunting the Needle in the Haystack: A Guide to Obtain Biologically Meaningful MicroRNA Targets. Karbiener, Michael; Glantschnig, Christina; Scheideler, Marcel // International Journal of Molecular Sciences;2014, Vol. 15 Issue 11, p20266 

    MicroRNAs (miRNAs) are endogenous small non-coding RNAs of ~23 nucleotides in length that form up a novel class of regulatory determinants, with a large set of target mRNAs postulated for every single miRNA. Thousands of miRNAs have been discovered so far, with hundreds of them shown to govern...

  • The Sequence and Structure Determine the Function of Mature Human miRNAs. Rolle, Katarzyna; Piwecka, Monika; Belter, Agnieszka; Wawrzyniak, Dariusz; Jeleniewicz, Jaroslaw; Barciszewska, Miroslawa Z.; Barciszewski, Jan // PLoS ONE;3/31/2016, Vol. 11 Issue 3, p1 

    Micro RNAs (miRNAs) (19–25 nucleotides in length) belong to the group of non-coding RNAs are the most abundant group of posttranscriptional regulators in multicellular organisms. They affect a gene expression by binding of fully or partially complementary sequences to the 3’-UTR of...

  • Do human transposable element small RNAs serve primarily as genome defenders or genome regulators? Lee, Kevin J.; Conley, Andrew B.; Lunyak, Victoria V.; Jordan, I. King // Mobile Genetic Elements (2159-2543);Jan/Feb2012, Vol. 2 Issue 1, p1 

    It is currently thought that small RNA (sRNA) based repression mechanisms are primarily employed to mitigate the mutagenic threat posed by the activity of transposable elements (TEs). This can be achieved by the sRNA guided processing of TE transcripts via Dicer-dependent (e.g., siRNA) or...

  • Argonaute proteins: functional insights and emerging roles. Meister, Gunter // Nature Reviews Genetics;Jul2013, Vol. 14 Issue 7, p447 

    Small-RNA-guided gene regulation has emerged as one of the fundamental principles in cell function, and the major protein players in this process are members of the Argonaute protein family. Argonaute proteins are highly specialized binding modules that accommodate the small RNA component - such...

  • Large-Scale Screens of miRNA-mRNA Interactions Unveiled That the 3′UTR of a Gene Is Targeted by Multiple miRNAs. Zhou, Peng; Xu, Weiyi; Peng, Xueling; Luo, Zhenhua; Xing, Qinghe; Chen, Xulin; Hou, Chengqian; Liang, Weihong; Zhou, Jianwen; Wu, Xiaoyan; Songyang, Zhou; Jiang, Songshan // PLoS ONE;Jul2013, Vol. 8 Issue 7, p1 

    Animal microRNA (miRNA) target prediction is still a challenge, although many prediction programs have been exploited. MiRNAs exert their function through partially binding the messenger RNAs (mRNAs; likely at 3′ untranslated regions [3′UTRs]), which makes it possible to detect the...

  • Translation-dependent displacement of UPF1 from coding sequences causes its enrichment in 3′ UTRs. Zünd, David; Gruber, Andreas R; Zavolan, Mihaela; Mühlemann, Oliver // Nature Structural & Molecular Biology;Aug2013, Vol. 20 Issue 8, p936 

    Recruitment of the UPF1 nonsense-mediated mRNA decay (NMD) factor to target mRNAs was initially proposed to occur through interaction with release factors at terminating ribosomes. However, recently emerging evidence points toward translation-independent interaction with the 3′...

  • Modelling Translation Initiation under the Influence of sRNA. Amman, Fabian; Flamm, Christoph; Hofacker, Ivo // International Journal of Molecular Sciences;Dec2012, Vol. 13 Issue 12, p16223 

    Bacterial small non-coding RNA (sRNA) plays an important role in post-transcriptional gene regulation. Although the number of annotated sRNA is steadily increasing, their functional characterization is still lagging behind. Various computational strategies for finding sRNA-mRNA interactions, and...

  • Small Non-Coding RNAs as Novel Therapeutics. Rossbach, M. // Current Molecular Medicine;Jun2010, Vol. 10 Issue 4, p361 

    RNA interference (RNAi), an evolutionarily conserved sequence-specific post-transcriptional gene silencing mechanism, is triggered by double-stranded RNA (dsRNA) that results in the degradation of homologous mRNA or in the inhibition of mRNA translation. The naturally occurring triggers for the...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics