Real Time Blood Testing Using Quantitative Phase Imaging

Pham, Hoa V.; Bhaduri, Basanta; Tangella, Krishnarao; Best-Popescu, Catherine; Popescu, Gabriel
February 2013
PLoS ONE;Feb2013, Vol. 8 Issue 2, p1
Academic Journal
We demonstrate a real-time blood testing system that can provide remote diagnosis with minimal human intervention in economically challenged areas. Our instrument combines novel advances in label-free optical imaging with parallel computing. Specifically, we use quantitative phase imaging for extracting red blood cell morphology with nanoscale sensitivity and NVIDIA’s CUDA programming language to perform real time cellular-level analysis. While the blood smear is translated through focus, our system is able to segment and analyze all the cells in the one megapixel field of view, at a rate of 40 frames/s. The variety of diagnostic parameters measured from each cell (e.g., surface area, sphericity, and minimum cylindrical diameter) are currently not available with current state of the art clinical instruments. In addition, we show that our instrument correctly recovers the red blood cell volume distribution, as evidenced by the excellent agreement with the cell counter results obtained on normal patients and those with microcytic and macrocytic anemia. The final data outputted by our instrument represent arrays of numbers associated with these morphological parameters and not images. Thus, the memory necessary to store these data is of the order of kilobytes, which allows for their remote transmission via, for example, the cellular network. We envision that such a system will dramatically increase access for blood testing and furthermore, may pave the way to digital hematology.


Related Articles

  • lesson of the month (1). Gupta, P Sen; Sharma, M; Timms, PM // Clinical Medicine;Jun2013, Vol. 13 Issue 3, p309 

    The article presents case studies related to laboratory samples with "unsuitable" laboratory comments which has diagnostic implications to the disease which affect the serum. It mentions that case of a 21-year-old man with type 2 diabetes mellitus and acute abdominal pain, and is diagnosed with...

  • Quantitative assessment of erythrocytes and leucocytes in CPD- A stored blood. Oluyombo, R.; Oluyombo, O.; Uchegbu, O. O.; Adegbamigbe, O.; Ayodele, O. E. // Biomedical Research (0970-938X);2013, Vol. 24 Issue 4, p503 

    Blood is stored to achieve a good post-transfusion survival. The ability of stored whole blood or blood components to fulfill their functions after transfusion can be studied during the period of storage. Transfusion of whole blood is currently being practiced in many centers in Nigeria despite...

  • Point-of-care hematology analyzers. Myers, J.D. // MLO: Medical Laboratory Observer;Aug2002, Vol. 34 Issue 8, p30 

    Provides information on several point-of-care hematology analyzers. Features of Portable Clinical Analyzer from i-STAT; Models of hematology analyzers from HemoCue Corp.; Mechanics of an analyzer from Careside.

  • Effect of Hematocrit on Exhaled Carbon Monoxide in Healthy Newborn Infants. Barak, Mila; Oron, Tal; Mimouni, Francis B.; Dollberg, Shaul; Littner, Yoav // Journal of Perinatology;Dec2005, Vol. 25 Issue 12, p784 

    OBJECTIVE:We tested the hypothesis that, the red blood cell (RBC) mass, estimated by hematocrit (HCT) or hemoglobin (Hb) level, influences the carbon monoxide (CO) production rate.STUDY DESIGN:The relationship between end tidal CO corrected for ambient carbon monoxide level (ETCOc) and the RBC...

  • Changes in the Hematological Profile of Holstein Primiparous in Periparturient Period and in Early to Mid Lactation. Găvan, Constantin; Retea, Costică; Motorga, Vergil // Scientific Papers: Animal Science & Biotechnologies / Lucrari St;Dec2010, Vol. 43 Issue 2, p244 

    Blood samples were collected from 25 primiparous Holstein cows divided in three groups (precalving, fresh cows and cows in early to mid lactation). The samples were analyzed for hematological parameters including red cell count (RBC), white cell count (WBC), hemoglobin concentration (HGC),...

  • Haemtological Parameters of Residents of Offa, KWARA State. Ojulari, L. S.; Abubakar, K. T.; Owoyele, B. V. // Bangladesh Journal of Medical Science;Apr2013, Vol. 12 Issue 2, p180 

    Objectives: This study aims to study some hematologic parameters, here, the packed cell volume (PCV), the red blood cell (RBC) count, hemoglobin concentration (HC) and white blood cell (WBC) count in residents of Offa in Kwara state, Nigeria Methods: Blood samples of healthy male and female Offa...

  • Haematological profile of messinese goat kids and their dams during the first month post-partum. Zumbo, Alessandro; Sciano, Salvatore; Messina, Vanessa; Casella, Stefania; di Rosa, Ambra Rita; Piccione, Giuseppe // Animal Science Papers & Reports;2011, Vol. 29 Issue 3, p223 

    The neonatal period represents a transition phase from the sheltered intrauterine to the exposed extrauterine environment. Changes in haematological profile in five pairs of twin kids and their dams were investigated over a month after the birth. During that period red blood cells (RBC), white...

  • ENDGAMES.  // BMJ: British Medical Journal;5/24/2014, Vol. 348 Issue 7959, p40 

    Several quiz about macrocytic anaemia, anteroposterior left vertebral angiogram, and the units of randomization in the clinical trial on the efficacy of a short course of oral prednisolone for acute asthma in children of school age are presented.

  • Instant Blood Counts. Charache, Samuel // Annals of Internal Medicine;Jun85, Vol. 102 Issue 6, p852 

    The article discusses the QBC hematology system which can estimate total leukocyte count, proportion of granulocytes, platelet count, and packed erythrocyte volume. It claims the device could save time between obtaining a blood sample and producing a report. Unbiased reports on the accuracy and...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics