TITLE

Production of LacZ Inducible T Cell Hybridoma Specific for Human and Mouse gp10025–33 Peptides

AUTHOR(S)
Cafri, Gal; Sharbi-Yunger, Adi; Tzehoval, Esther; Eisenbach, Lea
PUB. DATE
February 2013
SOURCE
PLoS ONE;Feb2013, Vol. 8 Issue 2, p1
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Identification and quantification of immunogenic peptides and tumor-derived epitopes presented on MHC-I molecules are essential for basic studies and vaccines generation. Although lymphocytes derived from transgenic mice can serve as sensitive detectors of processes of antigen presentation and recognition, they are not always available. The use of cell lines might be extremely useful. In this study, we generated a lacZ inducible CD8+ hybridoma (BUSA14) capable of recognizing both human and mouse gp10025–33 melanoma antigens presented on dendritic and tumor cell lines. This hybridoma expresses a variety of membranal T cell markers and secretes IL-2 and TNFα. Thus, BUSA14 offers a quantifiable, cheap and straightforward tool for studying peptide presentation by MHC-I molecules on the cell surface.
ACCESSION #
87623856

 

Related Articles

  • Enhancing In Silico Protein-Based Vaccine Discovery for Eukaryotic Pathogens Using Predicted Peptide-MHC Binding and Peptide Conservation Scores. Goodswen, Stephen J.; Kennedy, Paul J.; Ellis, John T. // PLoS ONE;Dec2014, Vol. 9 Issue 12, p1 

    Given thousands of proteins constituting a eukaryotic pathogen, the principal objective for a high-throughput in silico vaccine discovery pipeline is to select those proteins worthy of laboratory validation. Accurate prediction of T-cell epitopes on protein antigens is one crucial piece of...

  • Antigen-Presenting Cell (APC). Vohr, Hans-Werner // Encyclopedic Reference of Immunotoxicology;2005, p37 

    A definition of the term "antigen-presenting cell" is presented. It refers to the cells that can process protein antigens into peptides and express them on the surface for T cells stimulation. Such peptides are presented as a complex with class I or II major histocompatibility complex molecules....

  • The TCR/CD3 Complex: Opening the Gate to Successful Vaccination. Portolés, Pilar; Rojo, Jose M. // Current Pharmaceutical Design;10/1/2009, Vol. 15 Issue 28, p3290 

    The success of vaccination is directly or indirectly based on the specificity of antigen recognition by T lymphocytes, their efficient activation and expansion, and the generation of vaccine-specific effector and memory cells. These traits are largely dependent on the correct assembly and...

  • HLA-DO as the Optimizer of Epitope Selection for MHC Class II Antigen Presentation. Poluektov, Yuri O.; Kim, AeRyon; Hartman, Isamu Z.; Sadegh-Nasseri, Scheherazade // PLoS ONE;Aug2013, Vol. 8 Issue 8, p1 

    Processing of antigens for presentation to helper T cells by MHC class II involves HLA-DM (DM) and HLA-DO (DO) accessory molecules. A mechanistic understanding of DO in this process has been missing. The leading model on its function proposes that DO inhibits the effects of DM. To directly study...

  • Direct observation of ligand recognition by T cells. Irvine, Darrell J.; Purbhoo, Marco A.; Krogsgaard, Michelle; Davis, Mark M. // Nature;10/24/2002, Vol. 419 Issue 6909, p845 

    The activation of T cells through interaction of their T-cell receptors with antigenic peptide bound to major histocompatibility complex (MHC) on the surface of antigen presenting cells (APCs) is a crucial step in adaptive immunity. Here we use threedimensional fluorescence microscopy to...

  • Sustained and dynamic inositol lipid metabolism inside and outside the immunological synapse. Costello, Patrick S.; Gallagher, Maighread; Cantrell, Doreen A. // Nature Immunology;Nov2002, Vol. 3 Issue 11, p1082 

    T cell activation is triggered by several hours of contact with peptide-major histocompatibility (MHC) complexes on the surface of antigen-presenting cells (APCs). The nature and location of the sustained signal transduction pathways required for T cell activation are unknown. We show here that...

  • Making class II presentable. Schmid, Sandra L.; Jackson, Michael R. // Nature;5/12/1994, Vol. 369 Issue 6476, p103 

    Identifies a unique endosome-related subcellular compartment in antigen-presenting cells (APCs) where class II molecules of the histocompatibility complex (MHC) are loaded and presented. Importance of the compartment; Site and orchestration of the processing events; Challenge Resolution of class...

  • Antigen-recognition properties of murine ϒδ T cells. Chien, Y.-H.; Hampl, J. // Springer Seminars in Immunopathology;2000, Vol. 22 Issue 3, p239 

    Explores the antigen-recognition properties of murine gamma/delta T cells. Structural differences between murine T10/T22 ligands of peripheral gamma/delta T cells and classical major histocompatibility complex class I molecules; Possible contact sites of the G8 T cell receptor with T10;...

  • TCR Triggering by pMHC Ligands Tethered on Surfaces via Poly(Ethylene Glycol) Depends on Polymer Length. Ma, Zhengyu; LeBard, David N.; Loverde, Sharon M.; Sharp, Kim A.; Klein, Michael L.; Discher, Dennis E.; Finkel, Terri H. // PLoS ONE;Nov2014, Vol. 9 Issue 11, p1 

    Antigen recognition by T cells relies on the interaction between T cell receptor (TCR) and peptide-major histocompatibility complex (pMHC) at the interface between the T cell and the antigen presenting cell (APC). The pMHC-TCR interaction is two-dimensional (2D), in that both the ligand and...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics