Membrane Lipidome Reorganization Correlates with the Fate of Neuroblastoma Cells Supplemented with Fatty Acids

Bolognesi, Andrea; Chatgilialoglu, Alexandros; Polito, Letizia; Ferreri, Carla
February 2013
PLoS ONE;Feb2013, Vol. 8 Issue 2, p1
Academic Journal
Palmitic acid is known to be apoptotic for nervous cells but no data are available on membrane lipidome transformations occurring during its supplementation, although membrane lipids are clearly involved in the apoptotic signaling cascade. NB100 neuroblastoma cells were supplemented with palmitic acid and membrane fatty acids were isolated, derivatized and analysed by gas chromatography at defined time intervals. Parallely, cell viability, morphology, apoptosis, cPLA2 and caspase activations were checked. Interestingly, under 150 µM supplementation the incorporation of palmitic acid was accompanied by the specific release of arachidonic acid. This event was timely correlated with cPLA2 and caspases activations, and the time window of 60 minutes was envisaged for crucial membrane lipidome changes. The simultaneous addition of 50 µM oleic, 50 µM arachidonic and 150 µM palmitic acids to the cell cultures influenced membrane changes with suppression of caspase activation and maintenance of cell viability. These results highlight the role of the membrane asset with fatty acid remodeling and suggest the potential of lipid-based strategies for influencing cell response and fate in human diseases, such as neurodegenerative disorders or tumours.


Related Articles

  • Sonic Hedgehog pathway is essential for neuroblastoma cell proliferation and tumor growth. Xu, Lunshan; Wang, Xiangwei; Wan, Jianhua; Li, Tai; Gong, Xueyang; Zhang, Kui; Yi, Liang; Xiang, Zhonghuai; Xu, Minhui; Cui, Hongjuan // Molecular & Cellular Biochemistry;May2012, Vol. 364 Issue 1/2, p235 

    Accumulated evidence suggests a major role for the activation of the Sonic Hedgehog (SHH) signaling pathway in the development of neural crest stem cells that give rise to the sympathetic nervous system. We therefore investigated the involvement of SHH signaling in the pathogenesis of...

  • Polyunsaturated fats may slow growth of PCa.  // Urology Times;9/1/2005, Vol. 33 Issue 11, p44 

    Investigates the potential therapeutic value of polyunsaturated fatty acids in patients with prostate cancer by researchers from Chicago, Illinois. Analysis of measured levels of dietary fats in prostate cancer patients; Indications showing that men with less-advanced tumors had higher...

  • Basic aspects of tumor cell fatty acid-regulated signaling and transcription factors. Comba, Andrea; Lin, Yi-Hui; Eynard, Aldo; Valentich, Mirta; Fernandez-Zapico, Martín; Pasqualini, Marìa // Cancer & Metastasis Reviews;Dec2011, Vol. 30 Issue 3/4, p325 

    This article reviews the current knowledge and experimental research about the mechanisms by which fatty acids and their derivatives control specific gene expression involved during carcinogenesis. Changes in dietary fatty acids, specifically the polyunsaturated fatty acids of the ω-3 and...

  • A metabolic prosurvival role for PML in breast cancer. Carracedo, Arkaitz; Weiss, Dror; Leliaert, Amy K.; Bhasin, Manoj; de Boer, Vincent C. J.; Laurent, Gaelle; Adams, Andrew C.; Sundvall, Maria; Su Jung Song; Ito, Keisuke; Finley, Lydia S.; Egia, Ainara; Libermann, Towia; Gerhart-Hines, Zachary; Puigserver, Pere; Haigis, Marcia C.; Maratos-Flier, Elefteria; Richardson, Andrea L.; Schafer, Zachary T.; Pandolfi, Pier P. // Journal of Clinical Investigation;Sep2012, Vol. 122 Issue 9, p3088 

    Cancer cells exhibit an aberrant metabolism that facilitates more efficient production of biomass and hence tumor growth and progression. However, the genetic cues modulating this metabolic switch remain largely undetermined. We identified a metabolic function for the promyelocytic leukemia...

  • EDITORIAL. Achen, Marc G.; Stacker, Steven A. // Current Cancer Drug Targets;Sep2008, Vol. 8 Issue 6, p446 

    The article discusses various reports published within the issue, including one about cellular signaling systems involved in the communication between tumor cells and stromal components and another on the biology of the tumor stroma.

  • 2,2′,4,4′-Tetrabromodiphenyl ether promotes human neuroblastoma SH-SY5Y cells migration via the GPER/PI3K/Akt signal pathway. Tian, P-C; Wang, H-L; Chen, G-H; Luo, Q.; Chen, Z.; Wang, Y.; Liu, Y-F // Human & Experimental Toxicology;Feb2016, Vol. 35 Issue 2, p124 

    Neuroblastoma is the predominant tumor of early childhood. 2,2′,4,4′-Tetrabromodiphenyl ether (BDE-47) has the highest concentration among all polybrominated diphenyl ether (PBDE) congeners in human body, particularly for children. Considering that accumulating evidences showed...

  • Metastasis Suppressor microRNA-335 Targets the Formin Family of Actin Nucleators. Lynch, Jennifer; Meehan, Maria H.; Crean, John; Copeland, John; Stallings, Raymond L.; Bray, Isabella M. // PLoS ONE;Nov2013, Vol. 8 Issue 11, p1 

    MiRNAs can have pleiotropic effects by targeting multiple genes belonging to diverse signalling networks. Alternatively, miRNAs can enhance the potency of their cellular effects by targeting multiple genes within the same genetic pathway. Previously, we and others have demonstrated that miR-335...

  • Cell Survival Signalling through PPARδ and Arachidonic Acid Metabolites in Neuroblastoma. Bell, Emma; Ponthan, Frida; Whitworth, Claire; Westermann, Frank; Thomas, Huw; Redfern, Christopher P. F. // PLoS ONE;Jul2013, Vol. 8 Issue 7, p1 

    Retinoic acid (RA) has paradoxical effects on cancer cells: promoting cell death, differentiation and cell cycle arrest, or cell survival and proliferation. Arachidonic acid (AA) release occurs in response to RA treatment and, therefore, AA and its downstream metabolites may be involved in cell...

  • Hydrogen Sulfide-Induced Processing of the Amyloid Precursor Protein in SH-SY5Y Human Neuroblastoma Cells Involves the PI3-K/Akt Signaling Pathway. Zhang, Hua; Gao, Ying; Zhao, Feng-li; Qiao, Pei-feng; Yan, Yong // Cellular & Molecular Neurobiology;Mar2015, Vol. 35 Issue 2, p265 

    Hydrogen sulfide (H2S) has been recently categorized as a gasotransmitter, and it may be involved in the pathology of Alzheimer's disease. However, whether H2S induces amyloid precursor protein (APP) processing remains unknown. In the present study, we tested the ability of H2S to mediate APP...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics