TITLE

Recombinant Baculovirus Associated with Bilosomes as an Oral Vaccine Candidate against HEV71 Infection in Mice

AUTHOR(S)
Premanand, Balraj; Prabakaran, Mookkan; Kiener, Tanja K.; Kwang, Jimmy
PUB. DATE
February 2013
SOURCE
PLoS ONE;Feb2013, Vol. 8 Issue 2, p1
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: Human enterovirus 71 (HEV71) is one of the major pathogen responsible for hand, foot and mouth disease (HFMD). Currently no effective vaccine or antiviral drugs are available. Like poliovirus, EV71 is transmitted mainly by the feco-oral route. To date the majority of the studied EV71 vaccine candidates are administered parenterally. Injectable vaccines induce good systemic immunity but mucosal responses are often unsatisfactory, whereas mucosal vaccines provide both systemic and mucosal immunity. Therefore, oral immunization appears to be an attractive alternative to parenteral immunization. Methodology/Principal Findings: In this report, we studied the efficacy of an orally administered vaccine candidate developed using recombinant baculovirus displaying VP1 (Bac-VP1) in a murine model. Gastrointestinal delivery of Bac-VP1 significantly induced VP1-specific humoral (IgG) and mucosal (IgA) immune responses. Further, we studied the efficacy of the Bac-VP1 associated with bilosomes and observed that the Bac-VP1 associated with bilosomes elicited significantly higher immune responses compared to bilosomes non-associated with Bac-VP1. However, mice immunized subcutaneously with live Bac-VP1 had significantly enhanced VP1 specific serum IgG levels and higher neutralizing antibody titers compared with mice orally immunized with live Bac-VP1 alone or associated with bilosomes. Conclusion: Bilosomes have been shown to possess inherent adjuvant properties when associated with antigen. Therefore Bac-VP1 with bilosomes could be a promising oral vaccine candidate against EV71 infections. Thus, Bac-VP1 loaded bilosomes may provide a needle free, painless approach for immunization against EV71, thereby increasing patient compliance and consequently increasing vaccination coverage.
ACCESSION #
87623825

 

Related Articles

  • Adaptation of Enterovirus 71 to Adult Interferon Deficient Mice. Caine, Elizabeth A.; Partidos, Charalambos D.; Santangelo, Joseph D.; Osorio, Jorge E. // PLoS ONE;Mar2013, Vol. 8 Issue 3, p1 

    Non-polio enteroviruses, including enterovirus 71 (EV71), have caused severe and fatal cases of hand, foot and mouth disease (HFMD) in the Asia-Pacific region. The development of a vaccine or antiviral against these pathogens has been hampered by the lack of a reliable small animal model. In...

  • Amelioration of the Effects of Citrobacter rodentium Infection in Mice by Pretreatment with Probiotics. Johnson-Henry, Kathene C.; Nadjafi, Maral; Avitzur, Yaron; Mitchell, David J.; Bo-Yee Ngan; Galindo-Mata, Esther; Jones, Nicola L.; Sherman, Philip M. // Journal of Infectious Diseases;6/15/2005, Vol. 191 Issue 12, p2106 

    Background. Gitrobacter rodentium is a naturally occurring murine pathogen that causes colonic epithelial- cell hyperplasia, disrupts the colonic mucosa, and elicits a predominantly T helper 1 cellular immune response; it thereby serves as a model for the study of mechanisms of disease induced...

  • Anti-Nucleocapsid Protein Immune Responses Counteract Pathogenic Effects of Rift Valley Fever Virus Infection in Mice. Van Vuren, Petrus Jansen; Tiemessen, Caroline T.; Paweska, Janusz T. // PLoS ONE;2011, Vol. 6 Issue 9, p1 

    The known virulence factor of Rift Valley fever virus (RVFV), the NSs protein, counteracts the antiviral effects of the type I interferon response. In this study we evaluated the expression of several genes in the liver and spleen involved in innate and adaptive immunity of mice immunized with a...

  • Administration of E2 and NS1 siRNAs Inhibit Chikungunya Virus Replication In Vitro and Protects Mice Infected with the Virus. Parashar, Deepti; Paingankar, Mandar S.; Kumar, Satyendra; Gokhale, Mangesh D.; Sudeep, A. B.; Shinde, Sapana B.; Arankalle, V. A. // PLoS Neglected Tropical Diseases;Sep2013, Vol. 7 Issue 9, p1 

    Background: Chikungunya virus (CHIKV) has reemerged as a life threatening pathogen and caused large epidemics in several countries. So far, no licensed vaccine or effective antivirals are available and the treatment remains symptomatic. In this context, development of effective and safe...

  • Comparison of IRES and F2A-Based Locus-Specific Multicistronic Expression in Stable Mouse Lines. Hsiao Yun Chan.; Sivakamasundari., V.; Xing, Xing; Kraus, Petra; Sook Peng Yap; Ng, Patricia; Siew Lan; Lufkin, Lim Thomas // PLoS ONE;2011, Vol. 6 Issue 12, p1 

    Efficient and stoichiometric expression of genes concatenated by bi- or multi-cistronic vectors has become an invaluable tool not only in basic biology to track and visualize proteins in vivo, but also for vaccine development and in the clinics for gene therapy. To adequately compare, in vivo,...

  • Chitosan can stop or postpone the death of the suckling mice challenged with foot-and-mouth disease virus. Dong Li // Virology Journal;2010, Vol. 7, p125 

    In the study, a method called "hardening in liquid phase" for preparing chitosan granules with glutaraldehyde as crosslinker and Tween 80 as surfactant and paraffin liquid as dispersant was established. The chitosan granules were light yellow and insoluble in water or oil, but they swelled in...

  • Induction of Partial Protection against Foot and Mouth Disease Virus in Guinea Pigs by Neutralization with the Integrin β6-1 Subunit. Yan Zhang; Yingjun Sun; Fan Yang; Jianhong Guo; Jijun He; Qiong Wu; Weijun Cao; Lv Lv; Haixue Zheng; Zhidong Zhang // Viruses (1999-4915);Apr2013, Vol. 5 Issue 4, p1114 

    The mechanism by which the foot-and-mouth disease virus (FMDV) initiates infection of cells is thought to involve the attachment of the viral capsid to host integrins on the surface of target cells. However, the role of integrins in FMDV infection still needs to be fully understood, although it...

  • Detection of human enterovirus 71 and Coxsackievirus A16 in an outbreak of hand, foot, and mouth disease in Henan Province, China in 2009. Fan, Xingliang; Jiang, Jun; Liu, Yanjing; Huang, Xueyong; Wang, Pengzhi; Liu, Licheng; Wang, Junzhi; Chen, Weijun; Wu, Weili; Xu, Bianli // Virus Genes;Feb2013, Vol. 46 Issue 1, p1 

    During 2009, an outbreak of hand, foot, and mouth disease (HFMD) enrolled 490 people in Henan Province, causing the death of two children. In order to investigate the pathogens responsible for this outbreak and characterize their genetic characteristics, a total of 508 clinical specimens (stool,...

  • Antiviral Effect of Matrine against Human Enterovirus 71. Yajun Yang; Jinghui Xiu; Xu Zhang; Liangfeng Zhang; Kai Yan; Chuan Qin; Jiangning Liu // Molecules;Sep2012, Vol. 17 Issue 9, p10370 

    Human enterovirus 71, a member of the Picornaviridae family, is one of the major causative agent of hand, foot and mouth disease in children less than six years old. This illness has caused mortalities in large-scale outbreaks in the Asia-Pacific region in recent years. No vaccine or antiviral...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics