TITLE

Fluorescent Transgenic Zebrafish Tg(nkx2.2a:mEGFP) Provides a Highly Sensitive Monitoring Tool for Neurotoxins

AUTHOR(S)
Zhang, Xiaoyan; Gong, Zhiyuan
PUB. DATE
February 2013
SOURCE
PLoS ONE;Feb2013, Vol. 8 Issue 2, p1
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Previously a standard toxicological test termed as DarT (Danio rerio Teratogenic assay) using wild type zebrafish embryos has been established and it is widely applied in toxicological and chemical screenings. As an increasing number of fluorescent transgenic zebrafish lines with specific fluorescent protein expression specifically expressed in different organs and tissues, we envision that the fluorescent markers may provide more sensitive endpoints for monitoring chemical induced phenotypical changes. Here we employed Tg(nkx2.2a:mEGFP) transgenic zebrafish which have GFP expression in the central nervous system to investigate its potential for screening neurotoxic chemicals. Five potential neurotoxins (acetaminophen, atenolol, atrazine, ethanol and lindane) and one neuroprotectant (mefenamic acid) were tested. We found that the GFP-labeled ventral axons from trunk motoneurons, which were easily observed in live fry and measured for quantification, were a highly sensitive to all of the five neurotoxins and the length of axons was significantly reduced in fry which looked normal based on DarT endpoints at low concentrations of neurotoxins. Compared to the most sensitive endpoints of DarT, ventral axon marker could improve the detection limit of these neurotoxins by about 10 fold. In contrast, there was no improvement for detection of the mefenamic acid compared to all DarT endpoints. Thus, ventral axon lengths provide a convenient and measureable marker specifically for neurotoxins. Our study may open a new avenue to use other fluorescent transgenic zebrafish embryos/fry to develop sensitive and specific toxicological tests for different categories of chemicals.
ACCESSION #
87623782

 

Related Articles

  • Chlorpyrifos-Oxon Disrupts Zebrafish Axonal Growth and Motor Behavior. Yang, Dongren; Lauridsen, Holly; Buels, Kalmia; Chi, Lai-Har; La Du, Jane; Bruun, Donald A.; Olson, James R.; Tanguay, Robert L.; Lein, Pamela J. // Toxicological Sciences;May2011, Vol. 121 Issue 1, p146 

    Axonal morphology is a critical determinant of neuronal connectivity, and perturbation of the rate or extent of axonal growth during development has been linked to neurobehavioral deficits in animal models and humans. We previously demonstrated that the organophosphorus pesticide (OP)...

  • Effects of Di-n-butyl Phthalate and Diethyl Phthalate on Acetylcholinesterase Activity and Neurotoxicity Related Gene Expression in Embryonic Zebrafish. Xu, Hai; Shao, Xiaoling; Zhang, Zhen; Zou, Yanmin; Chen, Yao; Han, Songlei; Wang, Shasha; Wu, Xiangyang; Yang, Liuqing; Chen, Zhonglin // Bulletin of Environmental Contamination & Toxicology;Dec2013, Vol. 91 Issue 6, p635 

    In the present study, zebrafish embryos were used to assess the neurotoxicity of di- n-butyl phthalate (DBP), diethyl phthalate (DEP) and their mixture. Four-hour post-fertilization (hpf) zebrafish embryos were exposed to various concentrations of DBP, DEP and their mixture (DBP–DEP)...

  • The study of nuclear localization of amh and sox9a genes in male and female gonadal tissues of zebrafish. Karapetian, M.; Zaldastanishvili, E.; Zaalishvili, T.; Zaalishvili, G. // Biopolymers & Cell;2015 Suppl. 5, p34 

    Zebrafish (Danio rerio) is widely used model organism in numerous research fields. Different RNA FISH techniques have been optimized for zebrafish, however DNA FISH on tissue cryosections was never adapted for this organism. The main goals of present research were to optimize DNA FISH on tissue...

  • A GFP-Tagged Gross Deletion on Chromosome 1 Causes Malignant Peripheral Nerve Sheath Tumors and Carcinomas in Zebrafish. Astone, Matteo; Pizzi, Marco; Peron, Margherita; Domenichini, Alice; Guzzardo, Vincenza; Töchterle, Sonja; Tiso, Natascia; Rugge, Massimo; Meyer, Dirk; Argenton, Francesco; Vettori, Andrea // PLoS ONE;12/22/2015, Vol. 10 Issue 12, p1 

    Malignant peripheral nerve sheath tumors (MPNSTs) are highly aggressive soft-tissue sarcomas, characterized by complex karyotypes. The molecular bases of such malignancy are poorly understood and efficient targeted molecular therapies are currently lacking. Here we describe a novel zebrafish...

  • The Zebrafish Anillin-eGFP Reporter Marks Late Dividing Retinal Precursors and Stem Cells Entering Neuronal Lineages. Cepero Malo, Meret; Duchemin, Anne-Laure; Guglielmi, Luca; Patzel, Eva; Sel, Saadettin; Auffarth, Gerd U.; Carl, Matthias; Poggi, Lucia // PLoS ONE;1/20/2017, Vol. 12 Issue 1, p1 

    Monitoring cycling behaviours of stem and somatic cells in the living animal is a powerful tool to better understand tissue development and homeostasis. The tg(anillin:anillin-eGFP) transgenic line carries the full-length zebrafish F-actin binding protein Anillin fused to eGFP from a bacterial...

  • The Role of inab in Axon Morphology of an Identified Zebrafish Motoneuron. Van Ryswyk, Liesl; Simonson, Levi; Eisen, Judith S. // PLoS ONE;Feb2014, Vol. 9 Issue 2, p1 

    The ability of an animal to move and to interact with its environment requires that motoneurons correctly innervate specific muscles. Although many genes that regulate motoneuron development have been identified, our understanding of motor axon branching remains incomplete. We used...

  • Metabolism: Drug discovery goes for a swim. Choi, Jinkuk; Tontonoz, Peter // Nature Chemical Biology;Feb2013, Vol. 9 Issue 2, p68 

    The article discusses the relevance of whole organism screening in context of drug discovery. It further discusses the research work by scientist Gut and co-worker on the high throughput screening of zebra fish larvae for the identification of chemical modulators of glucose metabolism and...

  • Microarray analysis of gene expression during auditory hair cell regeneration in zebrafish (Danio rerio). Schuck, Julie B.; Smith, Michael E.; Xiaohong Li; Cooper, Nigel G. F. // BMC Bioinformatics;2008 Supplement 7, Vol. 9, Special section p1 

    Background Fishes are capable of regenerating sensory hair cells in the inner ear after overexposure to sound [1]. However, the time course and gene expression of auditory hair cell regeneration has not been established for zebrafish (Danio rerio). This study establishes a time course of cell...

  • Delta-Notch signaling and lateral inhibition in zebrafish spinal cord development. Appel, Bruce; Givan, Lee Anne; Eisen, Judith S. // BMC Developmental Biology;2001, Vol. 1, p13 

    Background: Vertebrate neural development requires precise coordination of cell proliferation and cell specification to guide orderly transition of mitotically active precursor cells into different types of post-mitotic neurons and glia. Lateral inhibition, mediated by the Delta-Notch signaling...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics