TITLE

Dimethyl Sulfoxide (DMSO) Exacerbates Cisplatin-induced Sensory Hair Cell Death in Zebrafish (Danio rerio)

AUTHOR(S)
Uribe, Phillip M.; Mueller, Melissa A.; Gleichman, Julia S.; Kramer, Matthew D.; Wang, Qi; Sibrian-Vazquez, Martha; Strongin, Robert M.; Steyger, Peter S.; Cotanche, Douglas A.; Matsui, Jonathan I.
PUB. DATE
February 2013
SOURCE
PLoS ONE;Feb2013, Vol. 8 Issue 2, p1
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Inner ear sensory hair cells die following exposure to aminoglycoside antibiotics or chemotherapeutics like cisplatin, leading to permanent auditory and/or balance deficits in humans. Zebrafish (Danio rerio) are used to study drug-induced sensory hair cell death since their hair cells are similar in structure and function to those found in humans. We developed a cisplatin dose-response curve using a transgenic line of zebrafish that expresses membrane-targeted green fluorescent protein under the control of the Brn3c promoter/enhancer. Recently, several small molecule screens have been conducted using zebrafish to identify potential pharmacological agents that could be used to protect sensory hair cells in the presence of ototoxic drugs. Dimethyl sulfoxide (DMSO) is typically used as a solvent for many pharmacological agents in sensory hair cell cytotoxicity assays. Serendipitously, we found that DMSO potentiated the effects of cisplatin and killed more sensory hair cells than treatment with cisplatin alone. Yet, DMSO alone did not kill hair cells. We did not observe the synergistic effects of DMSO with the ototoxic aminoglycoside antibiotic neomycin. Cisplatin treatment with other commonly used organic solvents (i.e. ethanol, methanol, and polyethylene glycol 400) also did not result in increased cell death compared to cisplatin treatment alone. Thus, caution should be exercised when interpreting data generated from small molecule screens since many compounds are dissolved in DMSO.
ACCESSION #
87623710

 

Related Articles

  • Aminoglycoside-Induced Hair Cell Death of Inner Ear Organs Causes Functional Deficits in Adult Zebrafish (Danio rerio). Uribe, Phillip M.; Sun, Huifang; Wang, Kevin; Asuncion, James D.; Wang, Qi; Chen, Chien-Wei; Steyger, Peter S.; Smith, Michael E.; Matsui, Jonathan I. // PLoS ONE;Mar2013, Vol. 8 Issue 3, p1 

    Aminoglycoside antibiotics, like gentamicin, kill inner ear sensory hair cells in a variety of species including chickens, mice, and humans. The zebrafish (Danio rerio) has been used to study hair cell cytotoxicity in the lateral line organs of larval and adult animals. Little is known about...

  • Transcriptional signature of accessory cells in the lateral line, using the Tnk1bp1:EGFP transgenic zebrafish line.  // BMC Developmental Biology;2012, Vol. 12 Issue 1, p6 

    The article focuses on the structural and molecular similarities between a fish and amphibian specific sensory organ. It states that zebrafish is used in this study for examining the development and biology of neuroepithelia of the inner ear. It mentions that the organs have hair cells also...

  • Inner Ear Undertakers. Grens, Kerry // Scientist;Sep2015, Vol. 29 Issue 8, p57 

    The article focuses on a study on live imaging the phagocytic activity of inner ear supporting cells in response to hair cell death by researcher Elyssa Monzack and colleagues, which was published in the 2015 issue of the journal; "Cell Death and Differentiation."

  • Microarray analysis of gene expression during auditory hair cell regeneration in zebrafish (Danio rerio). Schuck, Julie B.; Smith, Michael E.; Xiaohong Li; Cooper, Nigel G. F. // BMC Bioinformatics;2008 Supplement 7, Vol. 9, Special section p1 

    Background Fishes are capable of regenerating sensory hair cells in the inner ear after overexposure to sound [1]. However, the time course and gene expression of auditory hair cell regeneration has not been established for zebrafish (Danio rerio). This study establishes a time course of cell...

  • Effect of intratympanic dimethyl sulphoxide (DMSO) in an in vivo model of cisplatin-related ototoxicity. Roldán-Fidalgo, A.; Trinidad, A.; Rodríguez-Valiente, A.; García-Berrocal, J.; Millán, I.; Coronado, M.; Ramírez-Camacho, R. // European Archives of Oto-Rhino-Laryngology;Dec2014, Vol. 271 Issue 12, p3121 

    Although dimethyl sulfoxide (DMSO) is one of the most common solvents employed in otoprotection studies, its effect on the inner ear remains unknown. Only a few in vitro studies have addressed the effect of DMSO in cochlear cells. Up to the date, no in vivo functional studies have been reported....

  • Inner ear supporting cells protect hair cells by secreting HSP70. May, Lindsey A.; Kramarenko, Inga I.; Brandon, Carlene S.; Voelkel-Johnson, Christina; Roy, Soumen; Truong, Kristy; Francis, Shimon P.; Monzack, Elyssa L.; Fu-Shing Lee; Cunningham, Lisa L. // Journal of Clinical Investigation;Aug2013, Vol. 123 Issue 8, p3577 

    Mechanosensory hair cells are the receptor cells of hearing and balance. Hair cells are sensitive to death from exposure to therapeutic drugs with ototoxic side effects, including aminoglycoside antibiotics and cisplatin. We recently showed that the induction of heat shock protein 70 (HSP70)...

  • Neomycin-induced hair cell death and rapid regeneration in the lateral line of zebrafish (Danio rerio). Harris, Julie A.; Cheng, Alan G.; Cunningham, Lisa L.; MacDonald, Glen; Raible, David W.; Rubel, Edwin W // JARO - Journal of the Association for Research in Otolaryngology;Jun2003, Vol. 4 Issue 2, p219 

    Mechanoreceptive hair cells are extremely sensitive to aminoglycoside antibiotics, including neomycin. Hair cell survival was assessed in larval wild-type zebrafish lateral line neuromasts 4 h after initial exposure to a range of neomycin concentrations for 1 h. Each of the lateral line...

  • Role of Somatostatin Receptor-2 in Gentamicin-Induced Auditory Hair Cell Loss in the Mammalian Inner Ear. Brand, Yves; Radojevic, Vesna; Sung, Michael; Wei, Eric; Setz, Cristian; Glutz, Andrea; Leitmeyer, Katharina; Bodmer, Daniel // PLoS ONE;Sep2014, Vol. 9 Issue 9, p1 

    Hair cells and spiral ganglion neurons of the mammalian auditory system do not regenerate, and their loss leads to irreversible hearing loss. Aminoglycosides induce auditory hair cell death in vitro, and evidence suggests that phosphatidylinositol-3-kinase/Akt signaling opposes gentamicin...

  • Apaf-1 Inhibitors Protect from Unwanted Cell Death in In Vivo Models of Kidney Ischemia and Chemotherapy Induced Ototoxicity. Orzáez, Mar; Sancho, Mónica; Marchán, Sandra; Mondragón, Laura; Montava, Rebeca; Valero, Juan García; Landeta, Olatz; Basañez, Gorka; Carbajo, Rodrigo J.; Pineda-Lucena, Antonio; Bujons, Jordi; Moure, Alejandra; Messeguer, Angel; Lagunas, Carmen; Herrero, Carmen; Pérez-Payá, Enrique // PLoS ONE;Oct2014, Vol. 9 Issue 10, p1 

    Background: Excessive apoptosis induces unwanted cell death and promotes pathological conditions. Drug discovery efforts aimed at decreasing apoptotic damage initially targeted the inhibition of effector caspases. Although such inhibitors were effective, safety problems led to slow...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics