Visualization of Intrapulmonary Lymph Vessels in Healthy and Inflamed Murine Lung Using CD90/Thy-1 as a Marker

Kretschmer, Sarah; Dethlefsen, Ina; Hagner-Benes, Stefanie; Marsh, Leigh M.; Garn, Holger; König, Peter
February 2013
PLoS ONE;Feb2013, Vol. 8 Issue 2, p1
Academic Journal
Background: Lymphatic vessels play a pivotal role in fluid drainage and egress of immune cells from the lung. However, examining murine lung lymphatics is hampered by the expression of classical lymph endothelial markers on other cell types, which hinders the unambiguous identification of lymphatics. The expression of CD90/Thy-1 on lymph endothelium was recently described and we therefore examined its suitability to identify murine pulmonary lymph vessels under healthy and inflammatory conditions. Methodology/Principal Findings: Immunohistochemistry with a monoclonal antibody against CD90.2/Thy-1.2 on 200 µm thick precision cut lung slices labeled a vascular network that was distinct from blood vessels. Preembedding immunostaining and electron microscopy verified that the anti-CD90.2/Thy-1.2 antibody labeled lymphatic endothelium. Absence of staining in CD90.1/Thy-1.1 expressing FVB mice indicated that CD90/Thy-1 was expressed on lymph endothelium and labeling was not due to antibody cross reactivity. Double-labeling immunohistochemistry for CD90/Thy-1 and α-smooth muscle actin identified two routes for lymph vessel exit from the murine lung. One started in the parenchyma or around veins and left via venous blood vessels. The other began in the space around airways or in the space between airways and pulmonary arteries and left via the main bronchi. As expected from the pulmonary distribution of lymph vessels, intranasal application of house dust mite led to accumulation of T cells around veins and in the connective tissue between airways and pulmonary arteries. Surprisingly, increased numbers of T cells were also detected around intraacinar arteries that lack lymph vessels. This arterial T cell sheath extended to the pulmonary arteries where lymph vessels were located. Conclusions/Significance: These results indicate that CD90/Thy-1 is expressed on lymphatic endothelial cells and represents a suitable marker for murine lung lymph vessels. Combining CD90/Thy-1 labeling with precision cut lung slices allows visualizing the anatomy of the lymphatic system in normal and inflamed conditions.


Related Articles

  • Experimental evidence of cell dissemination playing a role in pathogenesis of IgA nephropathy in multiple lymphoid organs. Nakata, Junichiro; Suzuki, Yusuke; Suzuki, Hitoshi; Sato, Daisuke; Kano, Tatsuya; Horikoshi, Satoshi; Novak, Jan; Tomino, Yasuhiko // Nephrology Dialysis Transplantation;Feb2013, Vol. 28 Issue 2, p320 

    Background Since the pathogenesis of immunoglobulin A (IgA) nephropathy (IgAN) remains unclear, the rationale for current IgAN therapies is still obscure. Recent studies have shown that galactose-deficient IgA1 (GdIgA1) plays a critical role in the pathogenesis of IgAN and can be a non-invasive...

  • A Novel Anti-CEACAM5 Monoclonal Antibody, CC4, Suppresses Colorectal Tumor Growth and Enhances NK Cells-Mediated Tumor Immunity. Chaogu Zheng; Jing Feng; Di Lu; Ping Wang; Shu Xing; Coll, Jean-Luc; Dongling Yang; Xiyun Yan // PLoS ONE;2011, Vol. 6 Issue 6, p1 

    Carcinoembryonic antigen (CEA, CEACAM5, and CD66e) has been found to be associated with various types of cancers, particularly colorectal carcinoma, and developed to be a molecular target for cancer diagnosis and therapy. In present study, we generated a novel anti-CEACAM5 monoclonal antibody,...

  • PTP4A3 (PRL-3) expression correlate with lymphatic metastases in gastric cancer. Pryczynicz, Anna; Guzińska-Ustymowicz, Katarzyna; Chang, Xiao-Jia; Kiśluk, Joanna; Kemona, Andrzej // Folia Histochemica et Cytobiologica;2010, Vol. 48 Issue 4, p632 

    Many studies have proved that protein tyrosine phosphatase type IVAmember 3 (PTP4A3, PRL-3) plays a major role in the metastasis of gastric cancer, especially to local lymph nodes. The objective of the current study was to assess the expression of PTP4A3 in gastric cancer in correlation with...

  • Difference in abundance of blood and lymphatic capillaries in the murine epididymis. Hirai, Shuichi; Naito, Munekazu; Terayama, Hayato; Qu Ning; Miura, Masahiro; Shirakami, Gotaro; Itoh, Masahiro // Medical Molecular Morphology;Mar2010, Vol. 43 Issue 1, p37 

    It is well known that the epididymis is responsible for the transportation, storage, and maturation of spermatozoa, and that it has four segments: i.e., initial segment, caput, corpus, and cauda. Previous studies revealed the presence of a very dense network of blood capillaries only in the...

  • Expression analysis of the NDRG2 gene in mouse embryonic and adult tissues. Xiao-Lan Hu; Xin-Ping Liu; Yan-Chun Deng; Shu-Xin Lin; Lin Wu; Jian Zhang; Li-Feng Wang; Xiao-Bin Wang; Xia Li; Lan Shen; Yuan-Qiang Zhang; Li-Bo Yao // Cell & Tissue Research;Jun2006, Vol. 325 Issue 1, p67 

    N-myc downstream-regulated gene 2 (NDRG2) is believed to be involved in cell growth events. However, its exact function is still unknown. To elucidate the role of this gene, we used an anti-Ndrg2 monoclonal antibody in immunohistochemistry and immunofluorescence assays to analyze the expression...

  • Blocking CLEC14A-MMRN2 binding inhibits sprouting angiogenesis and tumour growth. Noy, P J; Lodhia, P; Khan, K; Zhuang, X; Ward, D G; Verissimo, A R; Bacon, A; Bicknell, R // Oncogene;11/19/2015, Vol. 34 Issue 47, p5821 

    We previously identified CLEC14A as a tumour endothelial marker. Here we show that CLEC14A is a regulator of sprouting angiogenesis in vitro and in vivo. Using a human umbilical vein endothelial cell spheroid-sprouting assay, we found CLEC14A to be a regulator of sprout initiation. Analysis of...

  • uPAR as Anti-Cancer Target: Evaluation of Biomarker Potential, Histological Localization, and Antibody-Based Therapy. Lund, Ida K.; Illemann, Martin; Thurison, Tine; Christensen, Ib J.; Høyer-Hansen, Gunilla // Current Drug Targets;Nov2011, Vol. 12 Issue 12, p1744 

    Degradation of proteins in the extracellular matrix is crucial for the multistep process of cancer invasion and metastasis. Compelling evidence has demonstrated the urokinase receptor (uPAR) and its cognate ligand, the urokinase plasminogen activator (uPA), to play critical roles in the...

  • A Murine, Bispecific Monoclonal Antibody Simultaneously Recognizing β-Glucan and MP65 Determinants in Candida Species. Zito, Andrea; Bromuro, Carla; Mandili, Giorgia; Chiani, Paola; Horenstein, Alberto L.; Malavasi, Fabio; Cauda, Roberto; Cassone, Antonio; Torosantucci, Antonella // PLoS ONE;2/9/2016, Vol. 11 Issue 2, p1 

    There is a real medical need of new diagnostic tools for the early recognition of invasive Candida infections. We exploited a rather simple and rapid redox methodology to construct a bispecific monoclonal antibody (bsmAb) that combines a monoclonal antibody (mAb) directed against...

  • Second-generation minimal physiologically-based pharmacokinetic model for monoclonal antibodies. Cao, Yanguang; Balthasar, Joseph; Jusko, William // Journal of Pharmacokinetics & Pharmacodynamics;Oct2013, Vol. 40 Issue 5, p597 

    Minimal physiologically-based pharmacokinetic (mPBPK) models provide a sensible modeling approach when fitting only plasma (or blood) data yielding physiologically-relevant PK parameters that may provide more practical value than parameters of mammillary models. We propose a second-generation...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics