TITLE

Functional Vascular Smooth Muscle-like Cells Derived from Adult Mouse Uterine Mesothelial Cells

AUTHOR(S)
Lachaud, Christian Claude; Pezzolla, Daniela; Domínguez-Rodríguez, Alejandro; Smani, Tarik; Soria, Bernat; Hmadcha, Abdelkrim
PUB. DATE
February 2013
SOURCE
PLoS ONE;Feb2013, Vol. 8 Issue 2, p1
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
In mammalian visceral organs, vascular smooth muscle cells (VSMCs) originate from an epithelial-to-mesenchymal transition (EMT) of embryonic mesothelial cells (MCs). The ability of adult MCs to recapitulate EMT and to acquire smooth muscle (SM) markers upon provasculogenic culture suggested they might retain embryonic vasculogenic differentiation potential. However, it remains unknown whether adult MCs-derived SM-like cells may acquire specific vascular SM lineage markers and the functionality of differentiated contractile VSMCs. Here, we describe how a gentle trypsinization of adult mouse uterine cords could selectively detach their outermost uterine mesothelial layer cells. As other MCs; uterine MCs (UtMCs) uniformly expressed the epithelial markers β-catenin, ZO-1, E-cadherin, CD54, CD29, and CK18. When cultured in a modified SM differentiation media (SMDM) UtMCs initiated a loss of epithelial characteristics and gained markers expression of EMT (Twist, Snail, and Slug), stem and progenitor (Nanog, Sox2, C-kit, Gata-4, Isl-1, and nestin), SM (α-SMA, calponin, caldesmon, SM22α, desmin, SM-MHC, and smoothelin-B) and cardiac (BMP2, BMP4, ACTC1, sACTN, cTnI, cTnT, ANF, Cx43, and MLC2a). UtMCs repeatedly subcultured in SMDM acquired differentiated VSM-like characteristics and expressed smoothelin-B in the typical stress-fiber pattern expression of contractile VSMCs. Relevantly, UtMCs-derived VSM-like cells could generate “mechanical force” to compact collagen lattices and displayed in diverse degree voltage (K+) and receptor (endothelin-1, oxytocin, norepinephrine, carbachol and vasopressin)-induced [Ca2+]i rises and contraction. Thus, we show for the first time that UtMCs could recapitulate in vitro differentiative events of early cardiovascular differentiation and transdifferentiate in cells exhibiting molecular and functional characteristics of VSMCs.
ACCESSION #
87623627

 

Related Articles

  • P748 Vascular smooth muscle cell proteome changes in a high fat diet animal model of atherogenesis. Rocchiccioli, S; Ucciferri, N; Viglione, F; Citti, L; Parodi, O; Pelosi, G; Trivella, MG; Cecchettini, A // Cardiovascular Research;Jul2014, Vol. 103 Issue suppl_1, pS137 

    Purpose: De-differentiation and phenotype changes in vascular smooth muscle cells (VSMCs) are hallmarks of vascular disorders such as atherosclerosis (ATS). High fat diet pigs were exploited as model of accelerated coronary ATS. In the present study, a gel-free proteomics approach was proposed...

  • Generation and Characterisation of Keratin 7 (K7) Knockout Mice Sandilands, Aileen; Smith, Frances J. D.; Lunny, Declan P.; Campbell, Linda E.; Davidson, Kirsty M.; MacCallum, Stephanie F.; Corden, Laura D.; Christie, Lesley; Fleming, Stewart; Lane, E. Birgitte; McLean, W. H. Irwin // PLoS ONE;May2013, Vol. 8 Issue 5, p1 

    Keratin 7 (K7) is a Type II member of the keratin superfamily and despite its widespread expression in different types of simple and transitional epithelia, its functional role in vivo remains elusive, in part due to the lack of any appropriate mouse models or any human diseases that are...

  • Functional Cooperation between Vitamin D Receptor and Runx2 in Vitamin D-Induced Vascular Calcification. Han, Min-Su; Che, Xiangguo; Cho, Gyoung-ho; Park, Hye-Ri; Lim, Kyung-Eun; Park, Na-Rae; Jin, Jung-Sook; Jung, Youn-Kwan; Jeong, Jae-Hwan; Lee, In-Kyu; Kato, Shigeaki; Choi, Je-Yong // PLoS ONE;Dec2013, Vol. 8 Issue 12, p1 

    The transdifferentiation of vascular smooth muscle cells (VSMCs) into osteoblast-like cells has been implicated in the context of vascular calcification. We investigated the roles of vitamin D receptor (Vdr) and runt-related transcription factor 2 (Runx2) in the osteoblastic differentiation of...

  • Dissecting out the Complex Ca2+-Mediated Phenylephrine-Induced Contractions of Mouse Aortic Segments. Fransen, Paul; Van Hove, Cor E.; Leloup, Arthur J. A.; Martinet, Wim; De Meyer, Guido R. Y.; Lemmens, Katrien; Bult, Hidde; Schrijvers, Dorien M. // PLoS ONE;Mar2015, Vol. 10 Issue 3, p1 

    L-type Ca2+ channel (VGCC) mediated Ca2+ influx in vascular smooth muscle cells (VSMC) contributes to the functional properties of large arteries in arterial stiffening and central blood pressure regulation. How this influx relates to steady-state contractions elicited by α1-adrenoreceptor...

  • Control of autophagy maturation by acid sphingomyelinase in mouse coronary arterial smooth muscle cells: protective role in atherosclerosis. Li, Xiang; Xu, Ming; Pitzer, Ashley; Xia, Min; Boini, Krishna; Li, Pin-Lan; Zhang, Yang // Journal of Molecular Medicine;May2014, Vol. 92 Issue 5, p473 

    Recent studies have indicated a protective role of autophagy in regulating vascular smooth muscle cells homeostasis in atherogenesis, but the mechanisms controlling autophagy, particularly autophagy maturation, are poorly understood. Here, we investigated whether acid sphingomyelinase...

  • Embryonic Stem Cells Are Redirected to Non-Tumorigenic Epithelial Cell Fate by Interaction with the Mammary Microenvironment. Boulanger, Corinne A.; Bruno, Robert D.; Mack, David L.; Gonzales, Monica; Castro, Nadia P.; Salomon, David S.; Smith, Gilbert H. // PLoS ONE;Apr2013, Vol. 8 Issue 4, p1 

    Experiments were conducted to redirect mouse Embryonic Stem (ES) cells from a tumorigenic phenotype to a normal mammary epithelial phenotype in vivo. Mixing LacZ-labeled ES cells with normal mouse mammary epithelial cells at ratios of 1∶5 and 1∶50 in phosphate buffered saline and...

  • Hypoxia Inducible Factor 3α Plays a Critical Role in Alveolarization and Distal Epithelial Cell Differentiation during Mouse Lung Development. Huang, Yadi; Kapere Ochieng, Joshua; Kempen, Marjon Buscop-van; Munck, Anne Boerema-de; Swagemakers, Sigrid; van IJcken, Wilfred; Grosveld, Frank; Tibboel, Dick; Rottier, Robbert J. // PLoS ONE;Feb2013, Vol. 8 Issue 2, p1 

    Lung development occurs under relative hypoxia and the most important oxygen-sensitive response pathway is driven by Hypoxia Inducible Factors (HIF). HIFs are heterodimeric transcription factors of an oxygen-sensitive subunit, HIFα, and a constitutively expressed subunit, HIF1β. HIF1α...

  • Cyclin I activates Cdk5 and regulates expression of Bcl-2 and Bcl-XL in postmitotic mouse cells. Brinkkoetter, Paul T.; Olivier, Paul; Wu, Jimmy S.; Henderson, Scott; Kroffi, Ronald D.; Pippin, Jeffrey W.; Hockenbery, David; Roberts, James M.; Shankland, Stuart J.; Krofft, Ronald D // Journal of Clinical Investigation;Oct2009, Vol. 119 Issue 10, p3089 

    Cyclin I is an atypical cyclin because it is most abundant in postmitotic cells. We previously showed that cyclin I does not regulate proliferation, but rather controls survival of podocytes, terminally differentiated epithelial cells that are essential for the structural and functional...

  • Organelle and Cellular Abnormalities Associated with Hippocampal Heterotopia in Neonatal Doublecortin Knockout Mice. Khalaf-Nazzal, Reham; Bruel-Jungerman, Elodie; Rio, Jean-Paul; Bureau, Jocelyne; Irinopoulou, Theano; Sumia, Iffat; Roumegous, Audrey; Martin, Elodie; Olaso, Robert; Parras, Carlos; Cifuentes-Diaz, Carmen; Francis, Fiona // PLoS ONE;Sep2013, Vol. 8 Issue 9, p1 

    Heterotopic or aberrantly positioned cortical neurons are associated with epilepsy and intellectual disability. Various mouse models exist with forms of heterotopia, but the composition and state of cells developing in heterotopic bands has been little studied. Dcx knockout (KO) mice show...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics