TITLE

Cell Differentiation of Pluripotent Tissue Sheets Immobilized on Supported Membranes Displaying Cadherin-11

AUTHOR(S)
Körner, Alexander; Deichmann, Christina; Rossetti, Fernanda F.; Köhler, Almut; Konovalov, Oleg V.; Wedlich, Doris; Tanaka, Motomu
PUB. DATE
February 2013
SOURCE
PLoS ONE;Feb2013, Vol. 8 Issue 2, p1
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Investigating cohesive tissue sheets in controlled cultures still poses a challenge since the complex intercellular interactions are difficult to mimic in in vitro models. We used supported lipid membranes functionalized by the adhesive part of the extracellular domain of the cell adhesion molecule cadherin-11 for the immobilization of pluripotent tissue sheets, the animal cap isolated from Xenopus laevis blastula stage embryos. Cadherin-11 was bound via histidine tag to lipid membranes with chelator head groups. In the first step, quantitative functionalization of the membranes with cadherin-11 was confirmed by quartz crystal microbalance and high energy specular X-ray reflectivity. In the next step, animal cap tissue sheets induced to neural crest cell fate were cultured on the membranes functionalized with cadherin-11. The adhesion of cells within the cohesive tissue was significantly dependent on changes in lateral densities of cadherin-11. The formation of filopodia and lamellipodia in the cohesive tissue verified the viability and sustainability of the culture over several hours. The expression of the transcription factor slug in externally induced tissue demonstrated the applicability of lipid membranes displaying adhesive molecules for controlled differentiation of cohesive pluripotent tissue sheets.
ACCESSION #
87623518

 

Related Articles

  • Surface Marker Epithelial Cell Adhesion Molecule and E-cadherin Facilitate the Identification and Selection of Induced Pluripotent Stem Cells. Chen, Hsin-Fu; Chuang, Ching-Yu; Lee, Wen-Chih; Huang, Hsiang-Po; Wu, Han-Chung; Ho, Hong-Nerng; Chen, Yu-Ju; Kuo, Hung-Chih // Stem Cell Reviews & Reports;Sep2011, Vol. 7 Issue 3, p722 

    The derivation of induced pluripotent stem cells (iPSCs) requires not only efficient reprogramming methods, but also reliable markers for identification and purification of iPSCs. Here, we demonstrate that surface markers, epithelial cells adhesion molecule (EpCAM) and epithelial cadherin...

  • Helical membrane protein conformations and their environment. Cross, Timothy; Murray, Dylan; Watts, Anthony // European Biophysics Journal;Oct2013, Vol. 42 Issue 10, p731 

    Evidence that membrane proteins respond conformationally and functionally to their environment is growing. Structural models, by necessity, have been characterized in preparations where the protein has been removed from its native environment. Different structural methods have used various...

  • The OpenPicoAmp: An Open-Source Planar Lipid Bilayer Amplifier for Hands-On Learning of Neuroscience. Shlyonsky, Vadim; Dupuis, Freddy; Gall, David // PLoS ONE;Sep2014, Vol. 9 Issue 9, p1 

    Understanding the electrical biophysical properties of the cell membrane can be difficult for neuroscience students as it relies solely on lectures of theoretical models without practical hands on experiments. To address this issue, we developed an open-source lipid bilayer amplifier, the...

  • Cell biology: A score for membrane fusion. Collins, Ruth N.; Zimmerberg, Joshua // Nature;6/25/2009, Vol. 459 Issue 7250, p1065 

    The article examines how cells orchestrate and perform intracellular membrane fusion. An experiment that uses a mix of 17 purified proteins and lipid bilayers to identify the process within the cells show that cells contain SNARE proteins link the internal membranes for fusion. It is responsible...

  • High-resolution analysis with novel cell-surface markers identifies routes to iPS cells. O'Malley, James; Skylaki, Stavroula; Iwabuchi, Kumiko A.; Chantzoura, Eleni; Ruetz, Tyson; Johnsson, Anna; Tomlinson, Simon R.; Linnarsson, Sten; Kaji, Keisuke // Nature;7/4/2013, Vol. 499 Issue 7456, p88 

    The generation of induced pluripotent stem (iPS) cells presents a challenge to normal developmental processes. The low efficiency and heterogeneity of most methods have hindered understanding of the precise molecular mechanisms promoting, and roadblocks preventing, efficient reprogramming....

  • Lipids: Leaflets out of order. Bucci, Mirella // Nature Chemical Biology;Feb2013, Vol. 9 Issue 2, p67 

    The article discusses the differences between the inner and the outer leaflet of the plasma membrane and also discusses the mechanism of interleaflet coupling and lipid diffusion in the membrane.

  • Sustained Levels of FGF2 Maintain Undifferentiated Stem Cell Cultures with Biweekly Feeding. Lotz, Steven; Goderie, Susan; Tokas, Nicolas; Hirsch, Sarah E.; Ahmad, Faizzan; Corneo, Barbara; Le, Sheila; Banerjee, Akhilesh; Kane, Ravi S.; Stern, Jeffrey H.; Temple, Sally; Fasano, Christopher A. // PLoS ONE;Feb2013, Vol. 8 Issue 2, p1 

    An essential aspect of stem cell culture is the successful maintenance of the undifferentiated state. Many types of stem cells are FGF2 dependent, and pluripotent stem cells are maintained by replacing FGF2-containing media daily, while tissue-specific stem cells are typically fed every 3rd day....

  • Maturation of Induced Pluripotent Stem Cell Derived Hepatocytes by 3D-Culture. Gieseck III, Richard L.; Hannan, Nicholas R. F.; Bort, Roque; Hanley, Neil A.; Drake, Rosemary A. L.; Cameron, Grant W. W.; Wynn, Thomas A.; Vallier, Ludovic // PLoS ONE;Jan2014, Vol. 9 Issue 1, p1 

    Induced pluripotent stem cell derived hepatocytes (IPSC-Heps) have the potential to reduce the demand for a dwindling number of primary cells used in applications ranging from therapeutic cell infusions to in vitro toxicology studies. However, current differentiation protocols and culture...

  • Survival of Primary Human Hepatocytes and Death of Induced Pluripotent Stem Cells in Media Lacking Glucose and Arginine. Tomizawa, Minoru; Shinozaki, Fuminobu; Sugiyama, Takao; Yamamoto, Shigenori; Sueishi, Makoto; Yoshida, Takanobu // PLoS ONE;Aug2013, Vol. 8 Issue 8, p1 

    Background: Tumorigenicity is an associated risk for transplantation of hepatocytes differentiated from human induced pluripotent stem (hiPS) cells. Hepatocytes express the enzymes galactokinase and ornithine transcarbamylase (OTC) to aid in their own survival. However, hiPS cells do not express...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics