Survey of single strand conformation polymorphism of kappa-casein gene in Alpine and Saanen goats in Iran

Nejhad, Rasoul Khatami; Yousefi, Soheil; Azari, Mojtaba Ahani
April 2013
Animal Science Papers & Reports;2013, Vol. 31 Issue 2, p173
Academic Journal
To study the genetic polymorphism of κ-casein (CSN3) gene as candidate gene for marker-assisted selection in different domestic livestock species, blood samples were randomly withdrawn from 34 Alpine and 42 Saanen goats. DNA was extracted from blood samples and a 416 bp fragment from exon 4 of the CSN3 gene was amplified by polymerase chain reaction (PCR). PCR products were analysed using single strand conformation polymorphism (SSCP) method. Results indicated that there were two (K1 and K2) conformational patterns with frequencies 0.91 and 0.09 for Alpine and 0.76 and 0.24 for Saanen goats, respectively. A Chi-square test confirmed Hardy-Weinberg equilibrium for the gene in both breeds.


Related Articles

  • Novel polymorphism of AA-NAT gene in Indian goat breeds differing in reproductive traits. Sharma, R.; Ahlawat, S.; Tantia, M. S. // Iranian Journal of Veterinary Research;2015, Vol. 16 Issue 4, p377 

    This is the first description of the polymorphisms of arylalkylamine-N-acetyltransferase (AA-NAT) gene in Indian goats with different reproductive traits (twinning percentage and age of sexual maturity). Based on the important role of AA-NAT in reproduction, it is considered as a possible...

  • MOLECULAR CHARACTERIZATION OF ASSAM HILL GOAT. Zaman, Galib Uz; Nahardeka, Naba; Laskar, Subimal; Ferdoci, Ali Mohomad; Chetri, Arun Jyoti // American Journal of Animal & Veterinary Sciences;2013, Vol. 8 Issue 2, p98 

    A total of 23 polymorphic microsatellite markers were used to evaluate genetic diversity and population structure in Assam Hill Goat (AHG). All the loci studied were polymorphic in nature. The number of observed alleles (Na) detected ranged from 2 to 10 with an overall mean of 4.9±2.220. A...

  • FECUNDITY GENE LINKED MICROSATELLITE MARKERS IN MALABARI GOATS. Seena, T. X.; Raghavan, K. C.; Mercy, K. A. // Biotechnology in Animal Husbandry;2012, Vol. 28 Issue 2, p295 

    The DNA samples from 120 Malabari goats were utilized to study the polymorphism of ovine fecundity genes related microsatellite markers in goats. A total of 15 alleles with 167-195 bp (base pairs) and 8 alleles with 98-114 bp were observed for loci BM1329 and TGLA68 with polymorphic information...

  • GM, KM Immunoglobulon Allotypes and Other Serum Genetic Markers (HP, GC, PI, and TF) among South... Dipierri, Jose E.; Alfaro, E. // Human Biology;Apr2000, Vol. 72 Issue 2, p305 

    Studies the GM, KM, HP, GC, PI and TF genetic systems in three populations living at different altitudes in San Salvador de Jujy, Argentina. Genetic markers used; Gene frequencies of the systems; Distribution of phenotype and haplotype/allele frequencies of each population.

  • Marker-assisted selection to increase effective population size by reducing Mendelian... Jinliang Wang; Hill, William G. // Genetics;Jan2000, Vol. 154 Issue 1, p475 

    Presents more general equations for effective population size of diploid species under random mating showing explicitly the two sources of inbreeding and genetic drift. Marker-assisted selection (MAS) methods to increase effective size for the whole genome; Restrictions on the efficiency of MAS.

  • Genetic Analysis of a Sicilian Population Using 15 Short Tandem Repeats. Calò, C.M.; Garofano, L.; Mameli, A.; Pizzamiglio, M.; Vona, G. // Human Biology;Apr2003, Vol. 75 Issue 2, p163 

    The genetic structure of the population of Alia (Sicily, Italy) was analyzed using 15 short tandem repeats: TPOX, D2S1338, D3S1358, FIBRA, D5S818, CSFIPO, D7S820, D8S1179, TH01, VWA, D13S317, D16S539, D18S51, D19S433, and D21S11. Two of these markers, D2S1338 and D19S433, have never before been...

  • Relationships Among Genetic Makeup, Active Ingredient Content, and Place of Origin of the Medicinal Plant Gastrodia Tuber. Tao, Jun; Luo, Zhi-yong; Msangi, Chikira Ismail; Shu, Xiao-shun; Wen, Li; Liu, Shui-ping; Zhou, Chang-quan; Liu, Rui-xin; Hu, Wei-xin // Biochemical Genetics;Feb2009, Vol. 47 Issue 1-2, p8 

    Gastrodia tuber and its component gastrodin have many pharmacological effects. The chemical fingerprints and gastrodin contents of eight Gastrodia populations were determined, and the genomic DNA polymorphism of the populations was investigated. Genetic distance coefficients among the...

  • ASSOCIATION OF POLYMORPHISM IN GROWTH AND DIFFERENTIATION FACTOR 5 GENE WITH OSTEOARTHRITIS KNEE. Mishra, Abhishek; Sanghi, Divya; Sharma, Amar Chandra; Raj, Saloni; Maurya, Shailendra Shanker; Avasthi, Sachin; Singh, Ajai; Parmar, Devendra; Srivastava, Rajeshwar Nath // American Journal of Biochemistry & Biotechnology;2013, Vol. 9 Issue 1, p1 

    The present study investigated to identify the association of polymorphism in GDF-5 gene with osteoarthritis in North Indian population. In a case-control study, 300 cases with knee osteoarthritis and an equal number of age and gender matched healthy controls were included. Cases were diagnosed...

  • Role of Transmitted Gag CTL Polymorphisms in Defining Replicative Capacity and Early HIV-1 Pathogenesis. Prince, Jessica L.; Claiborne, Daniel T.; Carlson, Jonathan M.; Schaefer, Malinda; Tianwei Yu; Lahki, Shabir; Prentice, Heather A.; Ling Yue; Vishwanathan, Sundaram A.; Kilembe, William; Goepfert, Paul; Price, Matthew A.; Gilmour, Jill; Mulenga, Joseph; Farmer, Paul; Derdeyn, Cynthia A.; Jiaming Tang; Heckerman, David; Kaslow, Richard A.; Allen, Susan A. // PLoS Pathogens;Nov2012, Vol. 8 Issue 11, Special section p1 

    Initial studies of 88 transmission pairs in the Zambia Emory HIV Research Project cohort demonstrated that the number of transmitted HLA-B associated polymorphisms in Gag, but not Nef, was negatively correlated to set point viral load (VL) in the newly infected partners. These results suggested...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics