Survey of single strand conformation polymorphism of kappa-casein gene in Alpine and Saanen goats in Iran

Nejhad, Rasoul Khatami; Yousefi, Soheil; Azari, Mojtaba Ahani
April 2013
Animal Science Papers & Reports;2013, Vol. 31 Issue 2, p173
Academic Journal
To study the genetic polymorphism of κ-casein (CSN3) gene as candidate gene for marker-assisted selection in different domestic livestock species, blood samples were randomly withdrawn from 34 Alpine and 42 Saanen goats. DNA was extracted from blood samples and a 416 bp fragment from exon 4 of the CSN3 gene was amplified by polymerase chain reaction (PCR). PCR products were analysed using single strand conformation polymorphism (SSCP) method. Results indicated that there were two (K1 and K2) conformational patterns with frequencies 0.91 and 0.09 for Alpine and 0.76 and 0.24 for Saanen goats, respectively. A Chi-square test confirmed Hardy-Weinberg equilibrium for the gene in both breeds.


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