TITLE

SIMULTANEOUS ESTIMATION OF SITAGLIPTIN AND SIMVASTATIN IN TABLET DOSAGE FORM BY A VALIDATED RP-HPLC METHOD

AUTHOR(S)
Raghava, K. A. S.; Rao, S. Venkat; Krishna, J. Anath; Satyanand, Y. S. V. R.
PUB. DATE
July 2012
SOURCE
International Journal of Pharmaceutical Sciences Review & Resear;Jul/Aug2012, Vol. 15 Issue 1, p41
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
An economical approach was used to develop and validate a rapid, specific, accurate, and precise reverse phase high performance liquid chromatographic (HPLC) method for the simultaneous estimation of Sitagliptin (SGP) and Simvastatin (SMS) in pharmaceutical dosage forms. The chromatographic separation was achieved on HPLC Column C18 75 × 4.6 mm, 5μ short column; Mobile phase (60:40) was established by using a buffer consisting of 0.05M Ammonium acetate (pH adjusted to 4.0) and Acetonitrile as organic solvent in a gradient program. The flow rate was 1.0 mL.min-1 and the detection wavelength was 253 nm. The limit of detection (LOD) for SMS and SGP was 0.305 and 1.156 μg.mL-1, respectively and their limit of quantification (LOQ) for SGP and SMS was 0.952 and 3.608 μg.mL-1, respectively. The proposed method was validated with respect to linearity, accuracy, precision, specificity, and robustness.
ACCESSION #
87366729

 

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