Basic Principles of MLC

Rambla-Alegre, Maria
January 2012
Chromatography Research International;2012, p1
Academic Journal
Micellar liquid chromatography (MLC) is an efficient alternative to conventional reversed-phase liquid chromatography with hydro-organic mobile phases. Almost three decades of experience have resulted in an increasing production of analytical applications. Current concern about the environment also reveals MLC as an interesting technique for "green" chemistry because it uses mobile phases containing 90% or more water. These micellar mobile phases have a low toxicity and are not producing hazardous wastes. The stationary phase is modified with an approximately constant amount of surfactant monomers, and the solubilising capability of the mobile phase is altered by the presence of micelles, giving rise to a great variety of interactions (hydrophobic, ionic, and steric) with major implications in retention and selectivity. From its beginnings in 1980, the technique has evolved up to becoming in a real alternative in some instances (and a complement in others) to classical RPLC with aqueousorganic mixtures, owing to its peculiar features and unique advantages. The addition of an organic solvent to the mobile phase was, however, soon suggested in order to enhance the low efficiencies and weak elution strength associated with the mobile phases that contained only micelles.


Related Articles

  • Analysis of Some Biogenic Amines by Micellar Liquid Chromatography. Malinowska, Irena; Stępnik, Katarzyna E. // Chromatography Research International;2012, p1 

    Micellar liquid chromatography (MLC) with the use of high performance liquid chromatography (HPLC) was used to determine some physicochemical parameters of six biogenic amines: adrenaline, dopamine, octopamine, histamine, 2-phenylethylamine, and tyramine. In this paper, an influence of...

  • Applications of Micellar Liquid Chromatography in Bioanalysis: A Review. Kalyankar, T. M.; Kulkarni, P. D.; Wadher, S. J.; Pekamwar, S. S. // Journal of Applied Pharmaceutical Science;Jan2014, Vol. 4 Issue 1, p128 

    Micellar liquid chromatography (MLC) is one of the very important analytical techniques. It contains mobile phase added with surfactants above its critical micellar concentration and the stationary phase is modified with surfactant monomers. So, micelles alter the solubilising capability of the...

  • Retention Behaviour in Micellar Liquid Chromatography. Rambla-Alegre, Maria // Chromatography Research International;2012, p1 

    Retention in micellar liquid chromatography is highly reproducible and can be modelled using empirical or mechanistic models with great accuracy to predict the retention changes when the mobile phase composition varies (surfactant and organic solvent concentrations), thus facilitating the...

  • Selectivity of Brij-35 in Micellar Liquid Chromatographic Separation of Positional Isomers. Memon, Najma; Shaikh, Huma I.; Solangi, Amber R. // Chromatography Research International;2012, p1 

    Implementation of Brij-35, a nonionic surfactant, as a mobile phase for separation of positional isomers is investigated. Chromolith C-18 SpeedROD is used as a stationary phase. The effect of surfactant and organic modifier (propanol) concentration on the separation of some selected isomers is...

  • THE METHOD OF STATIONARY PHASE FOR ONCE INTEGRATED GROUP. Vugdalić, Ramiz; Vajzović, Fikret // Publications de l'Institut Mathematique;2006, Issue 93, p73 

    We obtain a formula of decomposition for Φ(A) = A ∫ Rn S(f(x))φ(x) dx + ∫ Rn φ(x) dx using the method of stationary phase. Here (S(t))t∊R is once integrated, exponentially bounded group of operators in a Banach space X, with generator A, which satisfies the condition:...

  • New Fullerene-Based Stationary Phases for Gas Chromatography. Kartsova, L. A.; Makarov, A. A. // Journal of Analytical Chemistry;Aug2004, Vol. 59 Issue 8, p724 

    Regularities were revealed for the retention of different classes of organic compounds on stationary phases based on C60 and carbon nanotubes and on phases of mixed composition (C60 and dibenzo-24-crown-8, C60 and dibenzo-30-crown-10, and C60 and β-cyclodextrin) in gas chromatography. A...

  • Çevre Dostu Bir Analiz TekniÄŸi Olarak Misellar Sıvı Kromatografisi ve Gıda Analizlerinde Kullanımı. Şık, Bülent; Erkaymaz, Taner; Yıldız, Gizem // Academic Food Journal / Akademik GIDA;Jul2012, Vol. 10 Issue 3, p57 

    Micellar liquid chromatography (MLC) is a liquid chromatographic mode with mobile phases containing a surfactant above its critical micelle concentration. In MLC, surfactant concentrations in excess of the critical micelle concentration are used so that micelles are formed in the mobile phase....

  • Electrostatic ion chromatography using a carboxybetaine-type zwitterionic surfactant as the stationary phase. Hu, Wenzhi; Haddad, Paul R.; Tanaka, Kazuhiko; Hasebe, Kiyoshi // Analytical & Bioanalytical Chemistry;Jan2003, Vol. 375 Issue 2, p259 

    A carboxybetaine-type zwitterionic stationary phase obtained by immobilizing Mitsubishi Reagent EF-700 (C8F17SO2NHC3H6N+ (CH3)2-C2H4-COO–) onto a reversed-phase column was used for chromatographic separation of ions. When aqueous electrolyte solutions having higher pH values (>8) were...

  • Surfactant Modified/Mediated Thin-Layer Chromatographic Systems for the Analysis of Amino Acids. Bhawani, Showkat A.; Albishri, Hassan M.; Khan, Ziya Ahmad; Mohamad Ibrahim, Mohamad N.; Mohammad, A. // Journal of Analytical Methods in Chemistry;2013, p1 

    This review incorporates a large number of chromatographic systems modified by the surfactants. A large number of solvent systems and stationary phases are summarized in this paper. Three different kinds of surfactants (anionic, cationic, and nonionic) are used as modifiers for stationary phases...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics