TITLE

Atomic-resolution monitoring of protein maturation in live human cells by NMR

AUTHOR(S)
Banci, Lucia; Barbieri, Letizia; Bertini, Ivano; Luchinat, Enrico; Secci, Erica; Zhao, Yuguang; Aricescu, A Radu
PUB. DATE
May 2013
SOURCE
Nature Chemical Biology;May2013, Vol. 9 Issue 5, p297
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
We use NMR directly in live human cells to describe the complete post-translational maturation process of human superoxide dismutase 1 (SOD1). We follow, at atomic resolution, zinc binding, homodimer formation and copper uptake, and discover that copper chaperone for SOD1 oxidizes the SOD1 intrasubunit disulfide bond through both copper-dependent and copper-independent mechanisms. Our approach represents a new strategy for structural investigation of endogenously expressed proteins in a physiological (cellular) environment.
ACCESSION #
87008083

 

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