Employment of Electrochemical Methods for Assessment of the Maize (Zea mays L.) and Pea (Pisum sativum L.) Response to Treatment with Platinum(IV)

Mikulaskova, Hana; Rodrigo Merlos, Miguel Angel; Zitka, Ondrej; Kominkova, Marketa; Hynek, David; Adam, Vojtech; Beklova, Miroslava; Kizek, Rene
April 2013
International Journal of Electrochemical Science;Apr2013, Vol. 8 Issue 4, p4506
Academic Journal
Platinum group elements (PGEs) belong to the group of heavy metals, which represents potential high risk for the environment. They get into the environment mainly from the still increasing transport, however, they are used also in chemical, electrical and glass industry. In this study, we aimed our attention at using electrochemical methods as such differential pulse voltammetry and high performance liquid chromatography with electrochemical detection for determination of Pt(IV) content, amino acids, reduced (GSH) and oxidized (GSSG) glutathione levels, and phytochelatin synthase (PCS) activity in maize and pea plants treated with various doses of Pt(IV). The results revealed that amino acid levels, thiol contents and PCS activity increased markedly with the increasing concentration of Pt(IV). The amino acid content was the highest in maize and pea plants treated with 25 µM Pt(IV) in 8th and 12th days of treatment. GSH showed an 80% increase (compared to control) in plants treated with 100 µM after 12th days long exposure. The most significant increase of GSH was determined in roots pea treated with 25, 50 and 100 ?M. GSSG showed an increase of 100% (compared to control) in roots of pea treated with 100 µM after 8th days exposure. Different tendency was determined in the maize plants. The GSH/GSSG ratio was increased in shoots of maize treated with 25 µM at both sampling times. In both cultivars, PCS activity was increased, mainly due to 25, 50 and 100 µM of Pt(IV) exposure in roots and shoots. The comparison of the trends of slopes of dependences of total thiols and activity of PCS showed that maize roots and shoots were the obviously the same sensitive as shoots and roots of pea in thiol content but different situation was observed at PCS activity. Our data indicated that both species have different strategies to withstand the stress caused by Pt(IV).


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