Distinct Molecular Strategies for Hox-Mediated Limb Suppression in Drosophila: From Cooperativity to Dispensability/Antagonism in TALE Partnership

Sambrani, Nagraj; Hudry, Bruno; Maurel-Zaffran, Corinne; Zouaz, Amel; Mishra, Rakesh; Merabet, Samir; Graba, Yacine
March 2013
PLoS Genetics;Mar2013, Vol. 9 Issue 3, Special section p1
Academic Journal
The emergence following gene duplication of a large repertoire of Hox paralogue proteins underlies the importance taken by Hox proteins in controlling animal body plans in development and evolution. Sequence divergence of paralogous proteins accounts for functional specialization, promoting axial morphological diversification in bilaterian animals. Yet functionally specialized paralogous Hox proteins also continue performing ancient common functions. In this study, we investigate how highly divergent Hox proteins perform an identical function. This was achieved by comparing in Drosophila the mode of limb suppression by the central (Ultrabithorax and AbdominalA) and posterior class (AbdominalB) Hox proteins. Results highlight that Hox-mediated limb suppression relies on distinct modes of DNA binding and a distinct use of TALE cofactors. Control of common functions by divergent Hox proteins, at least in the case studied, relies on evolving novel molecular properties. Thus, changes in protein sequences not only provide the driving force for functional specialization of Hox paralogue proteins, but also provide means to perform common ancient functions in distinct ways.


Related Articles

  • Direct measurement of DNA affinity landscapes on a high-throughput sequencing instrument. Nutiu, Razvan; Friedman, Robin C; Luo, Shujun; Khrebtukova, Irina; Silva, David; Li, Robin; Zhang, Lu; Schroth, Gary P; Burge, Christopher B // Nature Biotechnology;Jul2011, Vol. 29 Issue 7, p659 

    Several methods for characterizing DNA-protein interactions are available, but none have demonstrated both high throughput and quantitative measurement of affinity. Here we describe 'high-throughput sequencing'-'fluorescent ligand interaction profiling' (HiTS-FLIP), a technique for measuring...

  • Evolution of Three Parent Genes and Their Retrogene Copies in Drosophila Species. O'Neill, Ryan S.; Clark, Denise V. // International Journal of Evolutionary Biology;2013, p1 

    Retrogenes form a class of gene duplicate lacking the regulatory sequences found outside of the mRNA-coding regions of the parent gene. It is not clear how a retrogene's lack of parental regulatory sequences affects the evolution of the gene pair. To explore the evolution of parent genes and...

  • Complete Dosage Compensation in Anopheles stephensi and the Evolution of Sex-Biased Genes in Mosquitoes. Xiaofang Jiang; Biedler, James K.; Yumin Qi; Brantley Hall, Andrew; Zhijian Tu // Genome Biology & Evolution;Jul2015, Vol. 7 Issue 7, p1914 

    Complete dosage compensation refers to hyperexpression of the entire X or Z chromosome in organisms with heterogametic sex chromosomes (XY male or ZW female) in order to compensate for having only one copy of the X or Z chromosome. Recent analyses suggest that complete dosage compensation, as in...

  • In Vivo Structure/Function Analysis of the Drosophila fat facets Deubiquitinating Enzyme Gene. Xin Chen; Fischer, Janice A. // Genetics;Dec2000, Vol. 156 Issue 4, p1829 

    Examines the function of the Drosophila fat facets (faf) proteins deubiquitinating enzyme (DUB) gene. Similarity of amino acid sequence regions; Use of ubiquitin-mediated proteolysis to regulate levels of critical regulatory proteins; Distinction of faf from a class of DUB; DNA sequences of faf...

  • Study of functional interaction between GAGA-containing sites from promoter regions of heat shock genes hsp26 and hsp70 in the model system of Drosophila melanogaster white gene. Melnikova, L. S.; Proskuryakov, K. A.; Georgiev, P. G. // Doklady Biochemistry & Biophysics;Oct2010, Vol. 434 Issue 1, p266 

    The article examines the ability of GAGA factor (GAF) to ensure distant interactions between the promoter of the white gene of Drosophila melanogaster and the yeast activator GAL4. DNA fragments of GAF are taken from the hsp26 and hsp70 gene promoters. It was found that the hsp70 fragment...

  • Steps to ensure accuracy in genotype and SNP calling from Illumina sequencing data. Qi Liu; Yan Guo; Jiang Li; Jirong Long; Bing Zhang; Yu Shyr // BMC Genomics;2012, Vol. 13 Issue Suppl 8, p1 

    Background: Accurate calling of SNPs and genotypes from next-generation sequencing data is an essential prerequisite for most human genetics studies. A number of computational steps are required or recommended when translating the raw sequencing data into the final calls. However, whether each...

  • Cooperative Binding of TEF-1 to Repeated GGAATG-Related Consensus Elements with Restricted Spatial Separation and Orientation. Jiang, Shi-Wen; Desai, Dinakar; Khan, Sohail; Eberhardt, Norman L. // DNA & Cell Biology;Aug2000, Vol. 19 Issue 8, p507 

    The human transcriptional enhancer factor (TEF) family includes TEF-1, TEF-3, TEF-4, and TEF-5. The TEFs share a highly conserved 68-amino acid TEA/ATTS DNA-binding domain, which binds to SV40 GTIIC (GGAATG), SphI (AGTATG), SphII (AGCATG), and muscle-specific M-CAT (GGTATG) enhansons. We...

  • DNA Sequence-Dependent Regulation of SF-1–Mediated Transcription. Brake, Paul B.; Bair, Susanna R.; Mellon, Synthia H. // DNA & Cell Biology;Mar2005, Vol. 24 Issue 3, p148 

    Rat P450c17 gene transcription is regulated by several nuclear factors, including steroidogenic factor-1 (SF-1), nerve growth factor-inducible protein B (NGF-IB, Nurr77), COUP-TF, SET, and Ku autoimmune antigen.A region of this gene,–447/–419, that mediates both basal and...

  • Quality of synthetic hexaploid wheat containing null alleles at Glu-A1 and Glu-B1 loci. HU, XINKUN; DAI, SHOUFEN; PU, ZHIEN; LIU, DENGCAI; PU, ZONGJUN; JIANG, JIAQI; WEI, YUMING; WU, BIHUA; LAN, XIUJIN; ZHENG, YOULIANG; YAN, ZEHONG // Journal of Genetics;Aug2013, Vol. 92 Issue 2, p241 

    Triticum turgidum ssp. dicoccon PI94668 and PI349045 were identified as containing null alleles at Glu-A1 and Glu-B1 loci in previous investigation. Sequencing of the respective HMW-GS genes Ax, Bx, Ay and By in both accessions indicated equal DNA lengths with gene silencing caused by 1 to 4...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics