TITLE

Detection of plasmid-mediated AmpC β-lactamase in Escherichia coli and Klebsiella pneumoniae

AUTHOR(S)
Yilmaz, N. O.; Agus, N.; Bozcal, E.; Oner, O.; Uzel, A.
PUB. DATE
January 2013
SOURCE
Indian Journal of Medical Microbiology;Jan-Mar2013, Vol. 31 Issue 1, p53
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Detecting plasmid-mediated AmpC (pAmpC) β-lactamase-producing organism is important for optimal infection control and providing accurate and effective treatment option for physicians. Oβjectives: The aim of this study was to investigate the prevalence of pAmpC β-lactamase and compare the results of βoronic acid (BA) disk test with other phenotypic tests detecting AmpC positive isolates. Materials and Methods: A total of 273 clinical isolates of Kleβsiella pneumoniae (n: 82) and Escherichia coli (n: 191) were analysed. The presence of pAmpC β-lactamase was determined βy BA disk test, cefoxitin (FOX) screening test, modifi ed three dimensional test (M3DT), and multiplex polymerase chain reaction (PCR). Pulsed-fi eld gel electrophoresis was performed to evaluate the genetic similarities βetween isolates. To detect extended spectrum β-lactamases (ESBL) in the presence of AmpC β-lactamase, ESBL confi rmation test was carried out with and without BA solution. Results: Of the 273 strains tested, 127 strains were found FOX resistant, 114 were positive βy M3DT, 108 were positive in BA disk test, and the multiplex PCR detected 24 pAmpC β-lactamase-positive isolate. The prevalence of AmpC-producing strains was 10.9% in E. coli and 3.6% in K. pneumoniae in the tested population βy PCR. CIT and MOX group genes were predominant type in these strains. Conclusion: These results emphasize that clinical laβoratories should consider testing the presence of pAmpC enzymes particularly in FOX-resistant isolates, and BA disk test will improve detection of this emerging resistance phenotype.
ACCESSION #
86867620

 

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