Activity of single ion channel proteins detected with a planar microstructure

Fertig, Niels; Klau, Michèle; George, Michael; Blick, Robert H.; Behrends, Jan C.
December 2002
Applied Physics Letters;12/16/2002, Vol. 81 Issue 25, p4865
Academic Journal
We present recordings of currents mediated by single ion channel proteins obtained using planar, microstructured glass chips. In these chips, pores with diameters of 1-2 µm are produced by ion track etching and are used for patch clamping instead of using the classical micropipette. Our results represent success in using such devices to record from single channels in cell membranes. The planar chip greatly enhances the accessibility of the ion channel containing membrane and can serve as a workbench for experiments on single ion channels using combinations of patch clamp current recording with other single molecule techniques.


Related Articles

  • channelopathy.  // Taber's Cyclopedic Medical Dictionary (2009);2009, Issue 21, p418 

    A definition of the medical term "channelopathy," which refers to a disease caused by a mutation in an ion channel in a cell membrane, is presented.

  • The Chemistry and Physics of Cell Signaling. Gwynne, Peter // Genomics & Proteomics;Jul/Aug2002, Vol. 2 Issue 6, p22 

    Examines the mechanism of cell signaling. Effects of peptides on ion channels; Use of fluorescent imaging techniques; Properties of cell membrane. INSET: IN LIVING COLOR.

  • Theoretical evaluation of cell membrane ion channel activation by applied magnetic fields. Pierre, Tim G. St.; Dobson, Jon // European Biophysics Journal;2000, Vol. 29 Issue 6, p455 

    Abstract This letter re-examines a recently published calculation of the forces exerted on a membrane ion channel by a cation passing through in the presence of an externally applied magnetic field. We show here, in contradiction to the originally published calculation, that the forces generated...

  • From glutathione transferase to pore in a CLIC. Cromer, Brett A.; Morton, Craig J.; Board, Philip G.; Parker, Michael W. // European Biophysics Journal;2002, Vol. 31 Issue 5, p356 

    Many plasma membrane chloride channels have been cloned and characterized in great detail. In contrast, very little is known about intracellular chloride channels. Members of a novel class of such channels, called the CLICs (chloride intracellular channels), have been identified over the last...

  • Transport of receptors, receptor signaling complexes and ion channels via neuropeptide-secretory vesicles. Bo Zhao; Hai-Bo Wang; Ying-Jin Lu; Jian-Wen Hu; Lan Bao; Xu Zhang // Cell Research;May2011, Vol. 21 Issue 5, p741 

    Stimulus-induced exocytosis of large dense-core vesicles (LDCVs) leads to discharge of neuropeptides and fusion of LDCV membranes with the plasma membrane. However, the contribution of LDCVs to the properties of the neuronal membrane remains largely unclear. The present study found that LDCVs...

  • Ion channels. Laniado, Marc E.; Abel, Paul D. // BMJ: British Medical Journal (International Edition);11/08/97, Vol. 315 Issue 7117, p1171 

    Editorials. Focuses on ion channels, the protein pores in the cell membrane that allows the passage of ions down their respective electrochemical gradients. What the ubiquitous presence of ion channels among cells of unicellular and multicellular organisms suggests; Classifications of ion...

  • Measurement of rapid in vitro response to an agonist of ion channel forming cell surface receptors. Marque, Jeffrey // Review of Scientific Instruments;Nov89, Vol. 60 Issue 11, p3503 

    We describe an instrument that is used for spatially homogeneous, submillisecond application of low-molecular-weight substrates (hundreds of daltons) in the neighborhood of a single biological cell and the measurement of the subsequent response of the cell to the substrate. Substrate application...

  • Functional localization of single active ion channels on the surface of a living cell. Korchev, Yuri E.; Negulyaev, Yuri A.; Edwards, Christopher R.W.; Vodyanoy, Igor; Lab, Max J. // Nature Cell Biology;Sep2000, Vol. 2 Issue 9, p616 

    The spatial distribution of ion channels in the cell plasma membrane has an important role in governing regional specialization, providing a precise and localized control over cell function. We report here a novel technique based on scanning ion conductance microscopy that allows, for the first...

  • VAMP2 interacts directly with the N terminus of Kv2.1 to enhance channel inactivation. Lvov, Anatoli; Chikvashvili, Dodo; Michaelevski, Izhak; Lotan, Ilana // Pflugers Archiv European Journal of Physiology;Sep2008, Vol. 456 Issue 6, p1121 

    Recently, we demonstrated that the Kv2.1 channel plays a role in regulated exocytosis of dense-core vesicles (DCVs) through direct interaction of its C terminus with syntaxin 1A, a plasma membrane soluble NSF attachment receptor (SNARE) component. We report here that Kv2.1 interacts with VAMP2,...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics