Guiding the migration of adherent cells by using optical micropatterns

Xiao, Jian-Long; Lu, De-Han; Lee, Chau-Hwang
March 2013
Applied Physics Letters;3/25/2013, Vol. 102 Issue 12, p123703
Academic Journal
We employed static and dynamic optical patterns to guide the motion of adherent cells. With the illumination intensity of 0.2 W/cm2 and propelling patterns moving at a speed of 2.8 μm/h, nearly 70% of the tested cells could be guided along a static optical pattern. We also demonstrated the cell guidance in an L-shaped optical channel. Comparing the result with only the static optical channel and that with both the static channel and moving optical pushes, we ascertained that the optical pushes placed at the start and turning points are necessary for effective optical cell guidance.


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