TITLE

Controlling one protein crystal growth by droplet-based microfluidic system

AUTHOR(S)
Yamaguchi, Hiroshi; Maeki, Masatoshi; Yamashita, Kenichi; Nakamura, Hiroyuki; Miyazaki, Masaya; Maeda, Hideaki
PUB. DATE
April 2013
SOURCE
Journal of Biochemistry;Apr2013, Vol. 153 Issue 4, p339
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The preparation of a single crystal is important for a detailed understanding of the structure of protein. However, the preparation of a suitable crystal for X-ray diffraction is often a drawback due to the complexity of the protein molecules and the limited fundamental understanding of the protein crystallization mechanism. In this study, we studied the crystallization mechanism in droplet that was prepared by the microfluidic chip. We found that the mechanism of crystal growth in droplet is different from that by a conventional microbatch method. One crystal was grown in one droplet by controlling droplet shape and droplet volume. In addition, the surface area in droplet affected the size of the obtained protein crystal and the number of crystal(s). The growth of the (110) and (101) faces of tetragonal crystal could be determined by studying one crystal formed within one droplet, indicating that the observation and evaluation of one crystal growth kinetics is easily carried out compared with the conventional method.
ACCESSION #
86428352

 

Related Articles

  • Structural and Spectroscopic Studies on Calcium Succinate Single Crystals. Binitha, M. P.; Pradyumnan, P. P. // AIP Conference Proceedings;2014, Vol. 1591, p1200 

    Calcium succinate crystals have been grown by gel aided solution growth technique. The structure of the crystal is solved by single crystal XRD analysis. The Crystal structure of the title compound is triclinic having space group P(1) Powder XRD studies confirmed the good crystalline nature of...

  • Growth and characterization of a promising ferroelectric relaxor material. Priya, R.; Bhagavannarayana, G.; Krishnan, S.; Das, S. Jerome // Archives of Applied Science Research;2010, Vol. 2 Issue 4, p111 

    Optical single crystals of ferroelectric trismethylammonium pentachloro barium dihydrate material were successfully grown by slow evaporation technique to a size of 20 X 17 X 3 mm3 within a period of 4 weeks. The crystal was subjected to single crystal X-ray diffraction, UV-Vis analysis,...

  • Crystal Growth and Structure of a New PbHNSO3 Crystal. Fukami, Takanori; Seino, Mitsuhiro; Nakasone, Keiko; Tahara, Shuta // International Journal of Chemistry;Feb2013, Vol. 5 Issue 1, p1 

    A new type of PbHNSO3 crystal has been grown by a gel method using agar-agar gel. TG-DTA and X-ray diffraction measurements have been performed on single crystal samples of PbHNSO3. It is found that the thermal decomposition of the compound starts at about 960 K. The space group (orthorhombic...

  • Studies on bulk growth, structural and microstructural characterization of 4-aminobenzophenone single crystal grown from vertical Bridgman technique. PRABHAKARAN, SP; RAMESH BABU, R; BHAGAVANNARAYANA, G; RAMAMURTHI, K // Bulletin of Materials Science;Feb2014, Vol. 37 Issue 1, p151 

    Bulk single crystal of 4-aminobenzophenone with a size of 25 mm dia. and 35 mm length has been grown by vertical Bridgman technique. The crystal system of the grown crystal was confirmed by X-ray diffraction analysis. Crystalline perfection was analysed by high resolution X-ray diffraction...

  • Preparation and Characterization of Lead-Free (K0.5Na0.5)NbO3-LiNbO3 and (K0.5Na0.5)NbO3-LiTaO3 Ferroelectric Single Crystals. Tao Chu; Chao He; Tailor, Hamel; Xifa Long // Crystals (2073-4352);Sep2014, Vol. 4 Issue 3, p295 

    Lead-free (K0.5Na0.5)NbO3-LiNbO3 (KNN-LN) and (K0.5Na0.5)NbO3-LiTaO3 (KNN-LT) ferroelectric single crystals, with the dimensions of 11 x 11 x 5 mm³ and 5 x 5 x 3 mm³, were grown successfully using the top-seeded solution growth (TSSG) method, respectively. The crystal structures were...

  • Crystal Growth, Optical and Dielectric Properties of CMTC - Grown by Gel Technique. kumari, P. Nisha Santha; Kalainathan, S.; Shakila, K.; Sivanandan, T. // International Journal of ChemTech Research;May/Jun2014, Vol. 6 Issue 3, p1663 

    Many of the bimetallic thiocyanate complexes have been found to exhibit excellent nonlinear optical properties. Single crystals of one such material Cadmium mercury thiocyanate CdHg(SCN)4 have been grown in silica gel using gel technique by the process of diffusion. The grown crystals subjected...

  • Studies on Synthesis, Growth, Structural, Optical Properties of Organic 8-Hydroxyquinolinium Succinate Single Crystals. Thirumurugan, R.; Anitha, K. // AIP Conference Proceedings;2014, Vol. 1591, p1226 

    8-hydroxyquinolinium succinate (8HQSU), an organic material has been synthesized and single crystals were grown by employing the technique of slow evaporation. The structure of the grown crystal was elucidated by using single crystal X-ray diffraction analysis. 8HQSU crystal belongs to the...

  • Crystal Growth, Structure analysis and Characterisation of 2 - (1, 3 - dioxoisoindolin - 2 - yl) Acetic Acid Single Crystal. Sankari, R. Siva; Perumal, Rajesh Narayana // AIP Conference Proceedings;2014, Vol. 1591, p1244 

    Single crystal of dielectric material 2 - (1, 3 - dioxoisoindolin - 2 - yl) acetic acid has been grown by slow evaporation solution growth method. The grown crystal was harvested in 25 days. The crystal structure was analyzed by Single crystal X - ray diffraction. UV-vis-NIR analysis was...

  • Morphology and kinetics of polymorphic transformations in KRbNO single crystals. Nasirov, V.; Bairamov, R.; Nasirov, E. // Crystallography Reports;Dec2015, Vol. 60 Issue 7, p1155 

    Polymorphic transformations in KRbNO single crystals have been investigated by optical microscopy and X-ray diffraction. The equilibrium temperature between modifications II and III of the crystal studied is determined to be T = 452 ± 0.5 K. It is found that polymorphic transformations are of...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics