TITLE

Aequorin-Based Luminescence Imaging Reveals Stimulus- and Tissue-Specific Ca2+ Dynamics in Arabidopsis Plants

AUTHOR(S)
Zhu, Xiaohong; Feng, Ying; Liang, Gaimei; Liu, Na; Zhu, Jian-Kang
PUB. DATE
March 2013
SOURCE
Molecular Plant (Oxford University Press / USA);Mar2013, Vol. 6 Issue 2, p444
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
An Aequorin-based Film Adhesive Seedling (FAS) Ca2+ recording system was developed for monitoring Ca2+ in response to various stimuli in Arabidopsis. This system revealed stimulus- and tissue-specific Ca2+ signatures in seedlings with a simple, sensitive, and robust Ca2+ recording.Calcium ion is a versatile second messenger for diverse cell signaling in response to developmental and environmental cues. The specificity of Ca2+-mediated signaling is defined by stimulus-elicited Ca2+ signature and downstream decoding processes. Here, an Aequorin-based luminescence recording system was developed for monitoring Ca2+ in response to various stimuli in Arabidopsis. With the simple, highly sensitive, and robust Ca2+ recording, this system revealed stimulus- and tissue-specific Ca2+ signatures in seedlings. Cellular Ca2+ dynamics and relationship to Aequorin-based Ca2+ recording were explored using a GFP-based Ca2+ indicator, which suggested that a synchronous cellular Ca2+ signal is responsible for cold-induced Ca2+ response in seedlings, whereas asynchronous Ca2+ oscillation contributes to osmotic stress-induced Ca2+ increase in seedlings. The optimized recording system would be a powerful tool for the identification and characterization of novel components in Ca2+-mediated stress-signaling pathways.
ACCESSION #
86428175

 

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