The RdDM Pathway Is Required for Basal Heat Tolerance in Arabidopsis

Popova, Olga V.; Dinh, Huy Q.; Aufsatz, Werner; Jonak, Claudia
March 2013
Molecular Plant (Oxford University Press / USA);Mar2013, Vol. 6 Issue 2, p396
Academic Journal
This work shows that a successful heat response depends on the integrity of epigenetic pathways and provides evidence that heat-dependent gene expression is influenced by closely located transposon sequences and read-through transcription.Heat stress affects epigenetic gene silencing in Arabidopsis. To test for a mechanistic involvement of epigenetic regulation in heat-stress responses, we analyzed the heat tolerance of mutants defective in DNA methylation, histone modifications, chromatin-remodeling, or siRNA-based silencing pathways. Plants deficient in NRPD2, the common second-largest subunit of RNA polymerases IV and V, and in the Rpd3-type histone deacetylase HDA6 were hypersensitive to heat exposure. Microarray analysis demonstrated that NRPD2 and HDA6 have independent roles in transcriptional reprogramming in response to temperature stress. The misexpression of protein-coding genes in nrpd2 mutants recovering from heat correlated with defective epigenetic regulation of adjacent transposon remnants which involved the loss of control of heat-stress-induced read-through transcription. We provide evidence that the transcriptional response to temperature stress, at least partially, relies on the integrity of the RNA-dependent DNA methylation pathway.


Related Articles

  • Site-specific methylation in gene coding region underlies transcriptional silencing of the Phytochrome A epiallele in Arabidopsis thaliana. Rangani, Gulab; Khodakovskaya, Mariya; Alimohammadi, Mohammad; Hoecker, Ute; Srivastava, Vibha // Plant Molecular Biology;May2012, Vol. 79 Issue 1/2, p191 

    DNA methylation in cytosine residues plays an important role in regulating gene expression. Densely methylated transgenes are often silenced. In contrast, several eukaryotic genomes express moderately methylated genes. These methylations are found in the CG context within the coding region (gene...

  • DNA methylation and gene expression in Mimulus guttatus. Colicchio, Jack M.; Fumihito Miura; Kelly, John K.; Takashi Ito; Hileman, Lena C. // BMC Genomics;Jul2015, Vol. 16 Issue 1, p1 

    Background: The presence of methyl groups on cytosine nucleotides across an organism's genome (methylation) is a major regulator of genome stability, crossing over, and gene regulation. The capacity for DNA methylation to be altered by environmental conditions, and potentially passed between...

  • A chromatin modifying enzyme, SDG8, is involved in morphological, gene expression, and epigenetic responses to mechanical stimulation. Cazzonelli, Christopher I.; Nisar, Nazia; Roberts, Andrea C.; Murray, Kevin D.; Borevitz, Justin O.; Pogson, Barry J. // Frontiers in Plant Science;Oct2014, Vol. 5, p1 

    Thigmomorphogenesis is viewed as being a response process of acclimation to short repetitive bursts of mechanical stimulation or touch. The underlying molecular mechanisms that coordinate changes in how touch signals lead to long-term morphological changes are enigmatic. Touch responsive gene...

  • Gene chip analysis of Arabidopsis thaliana genomic DNA methylation and gene expression in response to carbendazim. Li, Zhongai; Wang, Zicheng; Li, Suoping // Biotechnology Letters;Jun2015, Vol. 37 Issue 6, p1297 

    Objectives: To examine the effects of carbendazim on Arabidopsis genomic DNA methylation and gene expression. Results: Carbendazim caused widespread changes in gene loci methylation and gene expression. With 0.1 mM (D2) and 0.2 mM (D3) carbendazim, there were, respectively, 1522 and 2278...

  • Activation and Epigenetic Regulation of DNA Transposon nDart1 in Rice. Eun, Chang-Ho; Takagi, Kyoko; Park, Kyeung-Il; Maekawa, Masahiko; Iida, Shigeru; Tsugane, Kazuo // Plant & Cell Physiology;May2012, Vol. 53 Issue 5, p857 

    A large part of the rice genome is composed of transposons. Since active excision/reintegration of these mobile elements may result in harmful genetic changes, many transposons are maintained in a genetically or epigenetically inactivated state. However, some non-autonomous DNA transposons of...

  • Unusual Case of Apparent Hypermutation in Arabidopsis thaliana. Sasaki, Taku; Naumann, Ulf; Forai, Petar; Matzke, Antonius J. M.; Matzke, Marjori // Genetics;Dec2012, Vol. 192 Issue 4, p1271 

    The dms4 (defective in meristem silencing 4) mutant of Arabidopsis thaliana is unique in having defects in both RNA- directed DNA methylation (RdDM) and plant development. DMS4 is an evolutionarily conserved, putative transcription factor of the Iwr1 (interacts with RNA polymerase II) type. DMS4...

  • Genome-wide mapping of 5-hydroxymethylcytosine in three rice cultivars reveals its preferential localization in transcriptionally silent transposable element genes. Xi-liang Wang; Shu-hui Song; Yong-Sheng Wu; Yu-Li Li; Ting-ting Chen; Zhi-yuan Huang; Shuo Liu; Dunwell, Thomas L.; Pfeifer, Gerd P.; Dunwell, Jim M.; Wamaedeesa, Raheema; Ullah, Ihsan; Yinsheng Wang; Song-nian Hu // Journal of Experimental Botany;Nov2015, Vol. 66 Issue 21, p6651 

    5-Hydroxymethylcytosine (5hmC), a modified form of cytosine that is considered the sixth nucleobase in DNA, has been detected in mammals and is believed to play an important role in gene regulation. In this study, 5hmC modification was detected in rice by employing a dot-blot assay, and its...

  • DNA Methylation in Plants: Relationship to Small RNAs and Histone Modifications, and Functions in Transposon Inactivation. Saze, Hidetoshi; Tsugane, Kazuo; Kanno, Tatsuo; Nishimura, Taisuke // Plant & Cell Physiology;May2012, Vol. 53 Issue 5, p766 

    DNA methylation is a type of epigenetic marking that strongly influences chromatin structure and gene expression in plants and mammals. Over the past decade, DNA methylation has been intensively investigated in order to elucidate its control mechanisms. These studies have shown that small RNAs...

  • Divergent DNA methylation patterns associated with gene expression in rice cultivars with contrasting drought and salinity stress response. Garg, Rohini; Chevala, VVS Narayana; Shankar, Rama; Jain, Mukesh // Scientific Reports;10/9/2015, p1 

    DNA methylation is an epigenetic mechanism that play an important role in gene regulation in response to environmental conditions. The understanding of DNA methylation at the whole genome level can provide insights into the regulatory mechanisms underlying abiotic stress response/ adaptation. We...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics