TITLE

Neutron-encoded mass signatures for multiplexed proteome quantification

AUTHOR(S)
Hebert, Alexander S; Merrill, Anna E; Bailey, Derek J; Still, Amelia J; Westphall, Michael S; Strieter, Eric R; Pagliarini, David J; Coon, Joshua J
PUB. DATE
April 2013
SOURCE
Nature Methods;Apr2013, Vol. 10 Issue 4, p332
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
We describe a protein quantification method called neutron encoding that exploits the subtle mass differences caused by nuclear binding energy variation in stable isotopes. These mass differences are synthetically encoded into amino acids and incorporated into yeast and mouse proteins via metabolic labeling. Mass spectrometry analysis with high mass resolution (>200,000) reveals the isotopologue-embedded peptide signals, permitting quantification. Neutron encoding will enable highly multiplexed proteome analysis with excellent dynamic range and accuracy.
ACCESSION #
86416928

 

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