TITLE

Expression of HIV transgene aggravates kidney injury in diabetic mice

AUTHOR(S)
Mallipattu, Sandeep K; Liu, Ruijie; Zhong, Yifei; Chen, Ed Y; D'Agati, Vivette; Kaufman, Lewis; Ma'ayan, Avi; Klotman, Paul E; Chuang, Peter Y; He, John C
PUB. DATE
April 2013
SOURCE
Kidney International;Apr2013, Vol. 83 Issue 4, p626
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
With the widespread use of combination antiretroviral agents, the incidence of HIV-associated nephropathy has decreased. Currently, HIV-infected patients live much longer and often suffer from comorbidities such as diabetes mellitus. Recent epidemiological studies suggest that concurrent HIV infection and diabetes mellitus may have a synergistic effect on the incidence of chronic kidney disease. To address this, we determined whether HIV-1 transgene expression accelerates diabetic kidney injury using a diabetic HIV-1 transgenic (Tg26) murine model. Diabetes was initially induced with low-dose streptozotocin in both Tg26 and wild-type mice on a C57BL/6 background, which is resistant to classic HIV-associated nephropathy. Although diabetic nephropathy is minimally observed on the C57BL/6 background, diabetic Tg26 mice exhibited a significant increase in glomerular injury compared with nondiabetic Tg26 mice and diabetic wild-type mice. Validation of microarray gene expression analysis from isolated glomeruli showed a significant upregulation of proinflammatory pathways in diabetic Tg26 mice. Thus, our study found that expression of HIV-1 genes aggravates diabetic kidney disease.
ACCESSION #
86416890

 

Related Articles

  • Directed Engineering of a High-expression Chimeric Transgene as a Strategy for Gene Therapy of Hemophilia A. Doering, Christopher B.; Denning, Gabriela; Dooriss, Kerry; Gangadharan, Bagirath; Johnston, Jennifer M.; Kerstann, Keith W.; McCarty, David A.; Spencer, H. Trent // Molecular Therapy;Jul2009, Vol. 17 Issue 7, p1145 

    Human coagulation factor VIII (fVIII) is inefficiently biosynthesized in vitro and has proven difficult to express at therapeutic levels using available clinical gene-transfer technologies. Recently, we showed that a porcine and certain hybrid human/porcine fVIII transgenes demonstrate up to...

  • Metallothionein deficiency exacerbates diabetic nephropathy in streptozotocininduced diabetic mice. Hiromi Tachibana; Daisuke Ogawa; Norio Sogawa; Masato Asanuma; Ikuko Miyazaki; Naoto Terami; Takashi Hatanaka; Chikage Sato Horiguchi; Atsuko Nakatsuka; Jun Eguchi; Jun Wada; Hiroshi Yamada; Kohji Takei; Hirofumi Makino // Instructional Science;Jan2014, Vol. 41 Issue 1, pF105 

    Oxidative stress and inflammation play important roles in diabetic complications, including diabetic nephropathy. Metallothionein (MT) is induced in proximal tubular epithelial cells as an antioxidant in the diabetic kidney; however, the role of MT in renal function remains unclear. We therefore...

  • Mouse liver sinusoidal endothelium eliminates hiV-like Particles from Blood at a rate of 100 Million per Minute by a second-Order Kinetic Process. Mates, Jessica M.; Zhili Yao; Cheplowitz, Alana M.; Suer, Ozan; Phillips, Gary S.; Kwiek, Jesse J.; Rajaram, Murugesan V. S.; Jonghan Kim; Robinson, John M.; Ganesan, Latha P.; Anderson, Clark L. // Frontiers in Immunology;1/24/2017, Vol. 8, p1 

    We crafted human immunodeficiency virus (HIV)-like particles of diameter about 140 nm, which expressed two major HIV-1 proteins, namely, env and gag gene products, and used this reagent to simulate the rate of decay of HIV from the blood stream of BALB/c male mice. We found that most (~90%) of...

  • Genetic associations in DM nephropathy.  // Diabetes Digest;2011, Vol. 10 Issue 3, p161 

    The article focuses on the study by A. L. Mooyaart and colleagues which shows that 24 genetic variants are correlated with diabetic (DM) nephropathy.

  • Characterization of the Regulatory Region of Adra2c, the Gene Encoding the Murine α2C Adrenoceptor Subtype. Wen-Kwei Chen; Nan-Chi A. Chang; Yen-Hwa Chang; Kuo-Long Chang; Shinn-Chih Wu; Tzong-Shang Yang; Sheue-Mei Wu; Chien Chang, Alice // Journal of Biomedical Science;Nov/Dec2004, Vol. 11 Issue 6, p886 

    The 5′ flanking sequence (3,227 base pairs, bp) of the mouse Adra2c subtype gene was determined and characterized. The transcription start site was mapped to nucleotide ‘A’ of two initiator motifs in tandem array, i.e. 1,159 and 1,153 bp upstream from the initiation codon of...

  • Inducible, tightly regulated and non-leaky neuronal gene expression in mice. Delerue, Fabien; White, Michael; Ittner, Lars // Transgenic Research;Apr2014, Vol. 23 Issue 2, p225 

    The Tetracycline (Tet)-controlled inducible system is the most widely used reversible system for transgene expression in mice with over 500 lines created to date. Although this system has been optimized over the years, it still has limitations such as residual transgene expression when turned...

  • Analysis of the Temporal Requirement for Eda in Hair and Sweat Gland Development. Chang-Yi Cui; Kunisada, Makoto; Esibizione, Diana; Douglass, Eric G.; Schlessinger, David // Journal of Investigative Dermatology;Apr2009 Supplement 1, Vol. 129, p984 

    EDA signaling is important in skin appendage initiation. Its possible involvement in appendage subtype determination and postinduction stage appendage development, however, has not been studied systematically. To address these issues we manipulated Eda-A1 transgene expression in a...

  • 80. Pre-Existing Cytotoxic T-Lymphocyte (CTL) Responses to the AAV CAP Protein Do Not Interfere with AAV Transgene Expression in Mice. Siders, William M.; Kaplan, Johanne; Lukason, Michael; Shields, Jacqueline; Woodworth, Lisa; Wadsworth, Sam; Scaria, Abraham // Molecular Therapy;Jun2006, Vol. 13, pS34 

    The use of adeno-associated viral (AAV) vectors as a delivery method for gene replacement strategies is currently being explored in several clinical indications. AAV vectors do not express any viral proteins suggesting that they represent a less immunogenic delivery system. However, recent data...

  • The 3’-Jα Region of the TCRα Locus Bears Gene Regulatory Activity in Thymic and Peripheral T Cells. Kučerová-Levisohn, Martina; Knirr, Stefan; Mejia, Rosa I.; Ortiz, Benjamin D. // PLoS ONE;7/15/2015, Vol. 10 Issue 7, p1 

    Much progress has been made in understanding the important cis-mediated controls on mouse TCRα gene function, including identification of the Eα enhancer and TCRα locus control region (LCR). Nevertheless, previous data have suggested that other cis-regulatory elements may reside in the...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics