High resolution 4-dimension imaging of metanephric embryonic kidney morphogenesis

Clendenon, Sherry G; Ward, Heather H; Dunn, Kenneth W; Bacallao, Robert
April 2013
Kidney International;Apr2013, Vol. 83 Issue 4, p757
Academic Journal
High-resolution three-dimensional imaging of fixed embryonic kidney tissues has advanced considerably in the past decade. Here we developed a new process for imaging whole metanephric organ culture at cell resolution in three dimensions over time. This technique combines the use of the newly available generation of infrared-optimized long working distance, high numerical aperture objectives and multiphoton fluorescence microscopy with a new system for vital staining of metanephric organ cultures with bodipy ceramide. This allows all cells in the organ culture to be visualized over time, enabling detailed observation of tissue morphogenesis. Thus, our method offers a powerful new approach for visualizing and understanding early events in renal development and for extending observations made in genetically manipulated models.


Related Articles

  • Live-cell 3D super-resolution imaging in thick biological samples. Cella Zanacchi, Francesca; Lavagnino, Zeno; Perrone Donnorso, Michela; Del Bue, Alessio; Furia, Laura; Faretta, Mario; Diaspro, Alberto // Nature Methods;Dec2011, Vol. 8 Issue 12, p1047 

    We demonstrate three-dimensional (3D) super-resolution live-cell imaging through thick specimens (50-150 ?m), by coupling far-field individual molecule localization with selective plane illumination microscopy (SPIM). The improved signal-to-noise ratio of selective plane illumination allows...

  • A photoactivatable marker protein for pulse-chase imaging with superresolution. Fuchs, Jochen; Böhme, Susan; Oswald, Franz; Hedde, Per Niklas; Krause, Maike; Wiedenmann, Jörg; Nienhaus, G. Ulrich // Nature Methods;Aug2010, Vol. 7 Issue 8, p627 

    IrisFP is a photoactivatable fluorescent protein that combines irreversible photoconversion from a green- to a red-emitting form with reversible photoswitching between a fluorescent and a nonfluorescent state in both forms. Here we introduce a monomeric variant, mIrisFP, and demonstrate how its...

  • Super-resolution localization microscopy with photoactivatable fluorescent marker proteins. Hedde, Per; Nienhaus, G. // Protoplasma;Mar2014, Vol. 251 Issue 2, p349 

    Fluorescent proteins (FPs) have become popular imaging tools because of their high specificity, minimal invasive labeling and allowing visualization of proteins and structures inside living organisms. FPs are genetically encoded and expressed in living cells, therefore, labeling involves minimal...

  • Imaging Live Cells at the Nanometer-Scale with Single-Molecule Microscopy: Obstacles and Achievements in Experiment Optimization for Microbiology. Haas, Beth L.; Matson, Jyl S.; DiRita, Victor J.; Biteen, Julie S. // Molecules;Aug2014, Vol. 19 Issue 8, p12116 

    Single-molecule fluorescence microscopy enables biological investigations inside living cells to achieve millisecond- and nanometer-scale resolution. Although single-molecule-based methods are becoming increasingly accessible to non-experts, optimizing new single-molecule experiments can be...

  • Achievement of Superresolution in Optical-Thickness Measurement and Statistical Localization of Fluorophores in Holographic and Fluorescent Microscopy. Dudenkova, V.; Murav'eva, M.; Rybnikov, A.; Zakharov, Yu. // Radiophysics & Quantum Electronics;Jan2015, Vol. 57 Issue 8/9, p551 

    We present the concept of combining the holographic and localization fluorescent microscopy. The ways to optimize the optical schemes and image recording regimes in the process of combining such interferometric and statistical methods are developed, which makes it possible to study the dynamics...

  • Microbiology: Mapping microbes on the move. Eisenstein, Michael // Nature Methods;Dec2012, Vol. 9 Issue 12, p1145 

    The article offers information on a study related to the determination of locomotive behavior of microorganisms by using a low cost imaging strategy. It reports that differential dynamics microscopy (DDM) technique has been adopted by researchers to identify the cellular movement of...

  • The OPFOS Microscopy Family: High-Resolution Optical Sectioning of Biomedical Specimens. Buytaert, Jan A. N.; Descamps, Emilie; Adriaens, Dominique; Dirckx, Joris J. J. // Anatomy Research International;2012, p1 

    We report on the recently emerging (laser) light-sheet-based fluorescence microscopy field (LSFM). The techniques used in this field allow to study and visualize biomedical objects nondestructively in high resolution through virtual optical sectioning with sheets of laser light. Fluorescence...

  • Parallel super-resolution imaging. Rowlands, Christopher J; Yew, Elijah Y S; So, Peter T C // Nature Methods;Aug2013, Vol. 10 Issue 8, p709 

    The author discusses the developments in super-imaging techniques in context to reversible saturable optical fluorescence transitions (RESOLFT) approach developed by A. Chmyrov and colleagues. According to Chmyrov, the approach eliminates problems occurring in super-resolution imaging. He...

  • Super-Resolution Microscopy: A Virus' Eye View of the Cell. Grove, Joe // Viruses (1999-4915);Mar2014, Vol. 6 Issue 3, p1365 

    It is difficult to observe the molecular choreography between viruses and host cell components, as they exist on a spatial scale beyond the reach of conventional microscopy. However, novel super-resolution microscopy techniques have cast aside technical limitations to reveal a nanoscale view of...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics